EFFECT OF 1,5-BIS(3,5-DIMETHYLPYRAZOL-1-YL)-3-OXAPENTANE DIACETATOCOPPER ON THE STRUCTURAL AND FUNCTIONAL CHARACTERISTICS OF THE LIVER AND PANCREAS IN RATS UNDER EXPERIMENTAL CONDITIONS
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Keywords

infiltration
hemorrhage
karyopyknosis
karyorrhexis
karyolysis
total protein

How to Cite

Nofal, A. E., & Ovcharenko, N. D. (2016). EFFECT OF 1,5-BIS(3,5-DIMETHYLPYRAZOL-1-YL)-3-OXAPENTANE DIACETATOCOPPER ON THE STRUCTURAL AND FUNCTIONAL CHARACTERISTICS OF THE LIVER AND PANCREAS IN RATS UNDER EXPERIMENTAL CONDITIONS. Acta Biologica Sibirica, 2(2), 59-67. https://doi.org/10.14258/abs.v2i2.1346

Abstract

Various factors including chemical substances affecting on the human and animal organisms, causing a series of changes in the structural and metabolic processes. Chemical compounds cause a violation in the homeostatic regulatory systems, liver function and also influences the metabolism of hormones produced by the gonads in experimental animals. These studies suggest that, the intraperitoneal administration rats by 1,5-Bis(3,5-dimethylpyrazol-1-yl)-3-oxapentane diacetatocopper (dose 12 mg per kg of body weight for 6 weeks) led to disturbance of the normal structure of the liver and pancreas. This drug caused intensively neutrophilic infiltration around venous vessels with bleeding, endoplasmic vacuolation of most cells, karyopyknosis, karyorrhexis, and karyolysis, as well as, increasing the amount of binucleated cells. This treatment led to a reduction in the total protein content in the tissue of liver and pancreas, as well as activation of the apoptotic process in liver cells.

https://doi.org/10.14258/abs.v2i2.1346
PDF (Русский)

References

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REFERENCES

Bergamini E, De Tata, V, Cubeddu, T.L., Masiello, P., Pollera, M. (1987). Increased degradation in rat liver induced by antilipolytic agents: A model for studying autophagy and protein degradation in Liver. Exp. and Mol. Pathol., 46, 114 –122.

Donatia, A., Ventruria, A., Cavallinia, G., Masini, M., Vittorini, S., Chantret, I., Codogno, P., Bergamini, E. (2008). In vivo effect of an antilipolytic drug (3,5-dimethylpyrazole) on autophagic proteolysis and autophagy-related gene expression in rat liver. Biochem. and Biophys. Res. Commun., 366, 786–792.

Guo, L., Xiao, S., Gou, Y. (2004). Detection of bcl-2 and bax expression and bcl–2/JH fusion gene in intrahepatic cholangiocarcinoma. World J. Gastroenterol., 10(22), 3251–3254.

Hockenbery, D.M., Zutter, M., Hickey, W. (1991). Bcl-2 protein is topographically restricted in tissues characterized by apoptotic cell death. Proc. Nat. Acad. Sci., 88, 6961–6965.

Korsmeyer, S.J. (1992). Bcl-2 initiates a new category of oncogenes: regulators of cell death. Blood., 80, 879–886.

Lillie, R.D., Fulmer, H.M. (1976). Histopathological Technique and Practical Histochemistry. New York: Mc Graw Hill.

Locci, C.T., Masiello, P., Pollera, M. (1985). Effects of antilipolytic agents on rat liver peroxisomes and peroxisomai oxidative activities. Biochim. and Biophys. Acta, 839, 96–104.

Lu, Y., Cederbaum, A.I. (2006). Enhancement by pyrazole of lipopolysaccharide–induced liver injury in mice: role of cytochrome P450 2E1 and 2A5. Hepatol., 44(1), 263–274.

Ovsyanko, E.V., Lushnikova, E.L., Larionov, P.M. (2009). Immunohistochemical study for the expression of Bcl-2 family proteins in Walker 256 carcinosarcoma cells under the influence of cytostatic drugs.

Bull. Exp. Biol. Med., 148(4), 650–655.

Pearse, A.G.E. (1972). Histochemistry, Theoretical and Applied. London: Churchill Livingstone.

Potapov, A.S., Domina, G.A. Facile synthesis of flexible Bis(pyrazol-1-yl) alkane and related ligands in a superbasic medium. Eur. J. Org. Chem., 34, 5112–5116.

Potapov, A.S., Nudnova, E.A., Domina G.A., Kirpotina, L.N., Quinn, M.T., Khlebnikov, A.I., Schepetkin, I.A. (2009). Synthesis, characterization and potent superoxide dismutase-like activity of novel bis(pyrazole)-2,2-bipyridyl mixed ligand copper(II) complexes. Dalton Trans., 23, 4488–4498.

Sakr, S.A., El-Mesady, F.A., El-Desouki, N.I. (2002). Pyrethriod inhalation induced histochemical changes in the liver of Albino rats. The Sciences, 2(1), 24–28.

Semenuk, A.V. (1996). Structural and metabolic responses in the offspring of experimental animals undergoing the action of chemical compounds in embryogenesis. Thesis of Doctoral Dissertation. Irkutsk (in Russian).

Składziński, J., Beskid, M. (1978). Combined action of pyrazole and ethanol on a rat liver: histochemical and ultrastructural study. Fol. Histochem. and Cytochem., 16(1), 13–18.

Talman, P. (1979). Measurement of peripheral nerve regeneration in the rat and the effects of pyronin or hypothyroidism. Exp. Neurol., 65, 535–541.

Yamada, M., Murawaki, Y., Hirayama, C. (1987). Effects of ethanol feeding on collagen synthesizing and degrading enzymes in rat pancreas. Biochem. Pharmacol., 36(20), 3361–3364.

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