ISSR-PCR analysis of genetic variation in populations of Achillea L. species in southern part of Krasnoyarsk Territory and Republic of Khakassia
УДК 582.998.2:575.174:574.3
Abstract
ISSR-PCR analysis of populations of two species of the genus Achillea, growing in the southern part ofthe Krasnoyarsk Territory and the Republic of Khakassia, was carried out. The results of the study showed that a highlevel of intrapopulation variability is characteristic of Achillea asiatica and A. millefolium. Relatively low indicators ofgenetic variability are noted for populations Aa3 (A. asiatica) and Ak (A. kuprijanovii). The gene pool of all the studiedpopulations is characterized by a satisfactory state and can independently reproduce. Most of the genetic variability ofAchillea millefolium, A. asiatica, and A. kuprijanovii (74.7 %) accounts for the share of intrapopulation diversity, and25.3 % is a minor variation. The studied populations are characterized by a high level of differentiation and are dividedaccording to a systematic criterion.
Downloads
Metrics
References
Боронникова С. В. Молекулярно-генетический анализ генофондов редких и исчезающих растений Пермского края: дис.... докт. биол. наук. – Пермь, 2009. – 356 с.
Соколов П. Д. Растительные ресурсы СССР. Цветковые растения, их химический состав, использование: Семейство Asteraceae. – СПб.: Наука, 1993. – 349 с.
Степанов Н. В. Разнообразие тысячелистников (род Achillea L. – Asteraceae) в приенисейских Саянах // Вестник КрасГАУ. – Красноярск: Изд-во КрасГАУ, 2016. – № 6. – С. 31–38.
Doyle J. A rapid DNA isolation procedure for small quantities of fresh leaf tissue // Phytochemistry Bulletin, 1987. – № 19. – Pp. 11–15.
Farajpour M., Ebrahimi M., Amiri R., Golzari R., Sanjari S. Assesment of genetic diversity in Achillea millefolium accessions from Iran using ISSR marker // Biochemical systematic and ecology, 2012. – Vol. 43. – Pp. 73–79.
Gharibi S., Rahimmalek M., Mirlohi A., Majidi M. M., Tabatabaei B. E. S. Assesment of genetic diversity in Achillea millefolium subsp. millefolium and Achillea millefolium subsp. elbursensis using morphological and ISSR markers // Journal of Medical Plants Research, 2011. – Vol. 5(11). – Pp. 2413–2423.
Miller M. P. Tools for population genetic analysis (TFPGA) 1.3: a Windows program for the analysis of allozyme and molecular population genetic data // Computer software distributed by the author, 1997. – Pp. 30.
Nei M. Estimation of average heterozygosity and genetic distance from a small number of individuals // Genetics, 1978. – Vol. 89 (3). – Pp.583–590.
Poyaraz I. Comparison of ITS, RAPD and ISSR from DNA-based genetic diversity techniques // Comptes Rendus Biologies (May-June 2016). – Vol. 339, Iss. 5–6. – Pp. 171–178.
Rahimmalek M., Tabatabaey B. E. S., Arzani A., Khorrami M. Development and characterization of microsatellite markers for genomic analysis of yarrow (Achillea millefolium L.) // Genes & Genomics, 2011. – Vol. 33. – Pp. 475–483.
Wright S. Evolution and the genetics of population. Vol. 4. – Chicago, 1978. – 580 pp.
