STUDY OF PHENOLIC COMPOUNDS IN THE EXTRACTS OF BERRIES OF SORBUS AUCUPARIA
Abstract
The report presents the results of the study of phenolic complex of water, 40 and 95% acidified aqueous-ethanolic extracts of berries of mountain ash (Sorbus aucuparia L., family Rosaceae). The berries of mountain ash were collected by the authors in the natural habitats of the species in late August – early September 2014 in the territory of the Tomsk district Tomsk region; Angara district of the Irkutsk region, as well as the samples of raw materials of «Krasnogorskleksredstva». Prepared from rowan fruit hydroalcoholic extracts which are then separated into fractions using butanol, ethyl acetate, chloroform. Investigated crude fraction and fraction purified by column chromatography. The studies was conducted using chromatography on paper (PC), a thin layer of adsorbent (TLC), HPLC, HPLC-MS. HPLC was conducted on a chromatograph «Dionex Ultimate 3000» with UV - detector in the wavelength range from 254 to 330 nm. Separation was performed by a column with reversed-phase sorbent «Restek Pinnacle IIC18» (150×4,6 mm, particle size 5 µm), flow rate of mobile phase is 1 ml/min; temperature is 20 °C, mobile phase (50 ml of acetonitrile, 50 ml of 0,1% trifluoroacetic acid). HPLC-MS conducted on a chromatograph «Agilent Technologies 1100 Series LS / MSD» with UV - and mass selective detector. The stationary phase was a column with reversed phase «Zorbax XDB-C8 Rx-C18» (150×4,6 mm, particle size 5 µm, pore size 80 A), 2.3% of НСООН-МеОН (gradient 40 to 90% methanol with 5 to 10 minute) flow rate of mobile phase 1 ml/min.
Using methods of PC, TLC HPL, HPLC-MS by comparison with authentic substances were identified: cyanidin chloride, rutin, quercetin, isoquercitrin, baicalin, baicalein, apigenin, kaempferol, skutellarin, chlorogenic acid, vanillic acid, caffeic acid, coumaric acid, ferulic acid, cinnamic acid and salicylic acid. In the berries of Sorbus aucuparia baicalin and scutellarin identified for the first time. The results obtained allowed us to substantiate the choice of flavonoids and anthocyanins for the procedure of standardization of raw material and to enable methods of their determination in the project pharmacopeia monograph on the the berries of Sorbus aucuparia.Downloads
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