SPECTROPHOTOMETRIC DETERMINATION OF PHENOL COMPOUNDS FROM HERBS OF CORNFLOWER BLUE
Abstract
There was studied one- and three-fold solid-liquid extraction of natural phenolic compounds (NPC) (in terms of rutin and tannin) from various morphological parts of blue cornflower (Centaurea cyanus L.) on an ultrasonic device created in the research laboratory of the design bureau of the ISS (Voronezh). The optimal time of solid-liquid extraction at the ultrasonic unit has been determined, taking into account wetting and swelling of vegetable raw materials. The quantitative content of NPC (in terms of rutin and tannin) as a percentage of the total content in raw materials at the first stage is about 60%, at the second stage is 25%, and the third one is 18%. It was established that with threefold extraction, the yield of NPC from medicinal plant raw materials increases at the average of 8–10% compared with a one-time extraction. There is offered an improved technique of solid-liquid extraction on the ultrasound unit and UV spectrophotometric determination of NPC from different morphological parts of the blue cornflower. The technique is characterized by the absence of a systematic error, and the relative error at a confidence level of 0.95 does not exceed 5%. The duration of the analysis, taking into account the stage of wetting and osmotic swelling, triple extraction on the ultrasound unit and UV spectrophotometric determination of rutin and tannin in water-ethanol extracts is 40–45 minutes.
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Sokolov S.Ya., Zamotayev I.P. Spravochnik po lekarstvennym rasteniyam (Fitoterapiya). [Handbook of Medicinal Plants (Herbal Medicine)]. Moscow, 1990, 428 p. (in Russ.).
Shkol'nikova M.N., Aver'yanova Ye.V. Polzunovskiy vestnik, 2013, no. 4–4, pp. 168–172. (in Russ.).
Fedorova A.A. Rastitel'nyye resursy SSSR. Tsvetkovyye rasteniya, ikh khimicheskiy sostav, ispol'zovaniye. Semeystva Asteraceae. [Plant resources of the USSR. Flowering plants, their chemical composition, use. Families Asteraceae]. St. Petersburg, 1993. 326 p. (in Russ.).
Kompendium 2006 – lekarstvennyye preparaty. V 2 tomakh [Compendium 2006 – drugs. In 2 volumes], ed. V.N. Kovalenko, A.P. Viktorov. Kiyev, 2006, 2270 p. (in Russ.).
K'osev P.A. Polnyy spravochnik lekarstvennykh rasteniy. [A complete directory of medicinal plants]. Moscow, 2001, 992 p. (in Russ.).
Mashkovskiy M.D. Lekarstvennyye sredstva. [Medicines]. Moscow, 2005, 1200 p. (in Russ.).
Registr lekarstvennykh sredstv Rossii. Entsiklopediya lekarstv. [Register of medicines of Russia. Encyclopedia of drugs]. Moscow, 2003, 1440 p. (in Russ.).
Spravochnik Vidal' 2015. Lekarstvennyye preparaty v Rossii. [Vidal Handbook 2015. Medicines in Russia]. Moscow, 2015, 1480 p. (in Russ.).
Imanshahidi M., Hosseinzadeh H. Phytotherapy Research, 2006, vol. 20, pp. 427–437.
Jimenez-Zamora A., Delgado-Andrade C., Rufian-Henares J.A. Food Chemistry, 2016, vol. 199, pp. 339–346.
Lizzo M.R., Abouali M., Salehi P., Sonbolic A., Kananicnd M., Menichinia F., Tundisa R. Natural Product Research, 2014, vol. 28, no. 24, pp. 2278–2285.
Gosudarstvennaya farmakopeya. Rossiyskaya Federatsiya. XIV izdaniye. [The State Pharmacopoeia. Russian Federa-tion. XIV edition]. Moscow, 2018, vol. 2, pp. 367–369. (in Russ.).
Gosudarstvennaya farmakopeya. Rossiyskaya Federatsiya. XIV izdaniye. [The State Pharmacopoeia. Russian Federa-tion. XIV edition]. Мoscow, 2018, vol. 4, 1833 p. (in Russ.).
Podolina Ye.A., Khanina M.A., Rudakov O.B., Nebol'sin A.Ye. Vestnik VGU. Seriya: Biologiya, Khimiya, Farmatsi-ya, 2018, no. 2, pp. 28–35. (in Russ.).
Kuznetsov M.G., Khusayenov R.I. Izmel'cheniye rastitel'nogo syr'ya. [Grinding plant materials]. Kazan, 2012, 80 p. (in Russ.).
Ponomarev V.D. Ekstragirovaniye rastitel'nogo syr'ya. [Extraction of plant materials]. Moscow, 1979, 204 p. (in Russ.).
Gribova N.YU., Filippenko T.A., Nikolayevskiy A.N., Belaya N.I., Tsybulenko A.A. Zhurnal analiticheskoy khimii, 2008, vol. 63, no. 11, pp. 1137–1140. (in Russ.).
Gosudarstvennaya farmakopeya. Rossiyskaya Federatsiya. XIV izdaniye. [The State Pharmacopoeia. Russian Federa-tion. XIV edition]. Moscow, 2018, vol. 1, pp. 289–318. (in Russ.).
Phukan A., Chetia B., Handique J.G., Kardong D. National Academy Science Letters-India, 2016, vol.39, no. 2, pp. 99–102.
Podolina E.A., Rudakov O.B., Khorokhordina E.A., Kharitonova L.A. Journal of Analytical Chemistrry, 2008, vol. 63, no. 5, pp. 468–471.
Podolina Ye.A., Rudakova L.V., Rudakov O.B. Sorbtsionnyye i khromatograficheskiye protsessy, 2009, vol. 9, no. 2, pp. 177–190. (in Russ.).
Podolina E.A., Rudakov O.B., Thin F. V., Rudakova L.V. Jounal of Analytical Chemisty, 2010, vol. 65, no. 2, pp. 117–119.
Rudakov O.B., Khorokhrdina E.A., Podolina E.A., Kharitonova L.A. Russian Journal of Physical Chemistry, 2007, vol. 81, no. 12, pp. 2053–2058.
Minina S.A., Kaukhova I.Ye. Khimiya i tekhnologiya fitopreparatov. [Chemistry and technology of phytopreparations]. Moscow, 2004, 560 p. (in Russ.).
Rudakov O.B., Vostrikov I.A., Fedorov S.V., Filippov A.A., Selemenev V.F., Pridantseva A.A. Sputnik khromato-grafista. Metody zhidkostnoy khromatografii. [Chromatographer companion. Liquid chromatography methods]. Voro-nezh, 2004, 528 p. (in Russ.).
Zhukova O.L., Abramov A.A., Dargayeva T.D., Markaryan A.A. Vestnik Moskovskogo universiteta. Seriya 2. Khimi-ya, 2006, vol. 47, no. 5, pp. 342–345. (in Russ.).
Molchanov G.I. Ul'trazvuk v farmatsii. [Ultrasound in the pharmacy]. Moscow, 1980, 176 p. (in Russ.).
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