SELECTION OF OPTIMAL CONDITIONS FOR ACCUMULATION AND EXTRACTION OF PHENOLIC COM-POUNDS FROM BIOTECHNOLOGICAL RAW MATERIALS OF IRIS L. REPRESENTATIVES
UDC 615.322:582.579.2:581.192
Abstract
For optimal accumulation of biomass and phenolic compounds (flavonoids, tannins, xanthones, hydroxycinnamic acids) from Iris sibirica and Iris spuria in tissue culture, it is desirable to use a medium containing 1.0 to 2.5 µm BAP. The high content of cytokinin caused a stressful situation, at the first stage of which there was a mass formation of adventitious and axillary shoots, then with an increase in the concentration of the hormone, the reproduction coefficient decreased, the plants stopped reproducing, stopped growing and died. In extreme conditions, phenolic compounds are spent on the performance of protective functions, in connection with which there was a drop in their overall level in the tissues of irises.
For biotechnological raw materials of Iris L. representatives, the efficiency of subcritical sequential extraction was compared with traditional methods (in Soxlet type apparatus). In subcritical conditions have been removed more flavonoids and tannins: in fraction 96% ethanol of flavonoids and tannins – in 2 times; at a fraction of 60% ethanol flavonoids – 2 times, tanning – 3 times; at a fraction of the water of flavonoids in 3 times, tanning – 1.4 times. The water in the apparatus of soxlet extracted 1.3 times more hydroxycinnamic acids than in subcritical conditions.
The ratio of condensed and hydrolyzable tannins tannins in aeroponic raw material of Iris sibirica L. varieties Sterkh traditional extraction was determined as 13 : 1, the extraction in subcritical conditions 8 : 1.
Downloads
Metrics
References
Zaprometov M.N. Fenol'nyye soyedineniya i ikh rol' v zhizni rasteniya. [Phenolic compounds and their role in plant life]. Moscow, 1996, 45 p. (in Russ.).
Borisova G.G., Yermoshin A.A., Maleva M.A., Chukina N.V. Osnovy biokhimii vtorichnogo obmena rasteniy. [Fun-damentals of biochemistry of secondary metabolism of plants]. Yekaterinburg, 2014, 128 p. (in Russ.).
Tumova L., Rimakova J., Tuma J., Dusck J. Plant Cell Environ., 2006, vol. 52, pp. 454–458. DOI: 10.17221/3466-PSE.
Karppinen K., Hokkanen J., Tolonen A., Maltila S., Hohtola A. Phytochem., 2007, vol. 68, pp. 1038–1045. DOI: 10.1016/j.phytochem.2007.01.001.
Bazarnova N.G., Il'ichova T.N., Tikhomirova L.I., Sinitsina A.A. Khimiya Rastitel'nogo Syr'ya, 2016, no. 3, pp. 49–57. DOI: 10.14258/jcprm.2017011527. (in Russ.).
Tikhomirova L.I., Bazarnova N.G., Khalyavin I.A. Khimiya Rastitel'nogo Syr'ya, 2017, no. 2, pp. 119–126. DOI: 10.14258/jcprm.2017021517. (in Russ.).
Bazarnova N.G., Tikhomirova L.I., Sinitsyna A.A. Afanasenkova I.V. Khimiya Rastitel'nogo Syr'ya, 2017, no. 1, pp. 137–144. DOI: 10.14258/jcprm.2017042741. (in Russ.).
Tikhomirova L. In Vitro Cellular and Developmental Biology – Plant, 2017, vol. 53, no. 3, pp. 270–273. DOI: 10.1007/s11627-017-9817-6.
Gumerova Ye.A., Akulov A.N., Rumyantseva N.I. Mekhanizmy ustoychivosti rasteniy i mikroorganizmov k nebla-gopriyatnym usloviyam sredy. Sbornik materialov Godichnogo sobraniya Obshchestva fiziologov rasteniy Rossii, Vse-rossiyskoy nauchnoy konferentsii s mezhdunarodnym uchastiyem i shkoly molodykh uchenykh. [Mechanisms of re-sistance of plants and microorganisms to adverse environmental conditions. The collection of materials of the Annual Meeting of the Society of Plant Physiologists of Russia, the All-Russian Scientific Conference with international partic-ipation and the school of young scientists.]. Irkutsk, 2018, pp. 1229–1233. DOI: 10.31255/978-5-94797-319-8-1229-1233. (in Russ.).
Zagoskina N.V., Zaprometov M.N. Biologiya kul'tiviruyemykh kletok i biotekhnologiya rasteniy. [Cultured cell biology and plant biotechnology]. Moscow, 1991, pp. 32–35. (in Russ.).
Morini S., D’Onofrio C., Bellocchi G., Fisichella M. Plant Cell Tissue Org Cult., 2000, vol. 63, pp. 47–55. DOI: 10.1023/A:1006456919590.
Aremu A.O., Gruz J., Subrtová M., Szucová L., Dolezal K. J Plant Physiol., 2013, vol. 170, pp. 1303–1308. DOI: 10.1016/j.jplph.2013.04.008.
Minina S.A., Kaukhova I.Ye. Khimiya i tekhnologiya fitopreparatov. [Chemistry and technology of phytopreparations]. Moscow, 2004, 560 p. (in Russ.).
Kalinin F.L., Sariatskaya V.V., Polishchuk V.Ye. Metody kul'tury tkaney v fiziologii i biokhimii rasteniy. [Methods of tissue culture in the physiology and biochemistry of plants]. Kiev, 1980, 488 p. (in Russ.).
Murashige T., Skoog F. Physiol. Plant, 1962, vol. 15, no. 4, p. 473.
Vetrova Ye.V., Maksimenko Ye.V., Borisenko S.N., Lekar' A.V., Borisenko N.I., Minkin V.I. Sverkhkriticheskiye flyuidy. Teoriya i praktika. 2016, vol. 11, no. 4, pp. 73–79. (in Russ.).
OFS.1.5.3.0006.15. Opredeleniye soderzhaniya ekstraktivnykh veshchestv v lekarstvennom rastitel'nom syr'ye i lekarstvennykh rastitel'nykh preparatakh. [OFS.1.5.3.0006.15. Determination of the content of extractive substances in medicinal plant materials and medicinal herbal preparations]. Moscow, 2015, 5 p. (in Russ.).
Muzychkina R.A. Tekhnologiya proizvodstva i analiz fitopreparatov. [Production technology and analysis of herbal remedies]. Almaty, 2011, 360 p. (in Russ.).
OFS.1.5.3.0008.18. Opredeleniye soderzhaniya dubil'nykh veshchestv v lekarstvennom rastitel'nom syr'ye i le-karstvennykh rastitel'nykh preparatakh. [OFS.1.5.3.0008.18. Determination of the content of tannins in medicinal plant materials and herbal medicines]. Moscow, 2018, 4 p. (in Russ.).
Kukushkina T.A., Zinner N.S., Vysochina G.I., Sviridova T.P. Khimiya Rastitel'nogo Syr'ya, 2011, no. 3, pp. 113–116. (in Russ.).
Bubenchikova V.N., Starchak Yu.A. Voprosy biologicheskoy, meditsinskoy i farmatsevticheskoy khimii, 2015, no. 5, pp. 14–18. (in Russ.).
Pasqua G., Avato P., Monacelli B., Santamaria A.R., Argentieri M.P. Plant Science, 2003, vol. 165, pp. 977–982. DOI: 10.1016/S0168-9452(03)00275-9.
Stafford A. Plant Cell and Tissue Culture. Open University Press, Buckingham, UK, 1991, pp 124–238.
Palacioa L., Canterob J.J., Cusidoc R.M., Goleniowskia M.E. Plant Sci., 2012, vol. 193–194, pp. 1–7. DOI: 10.1016/j.plantsci.2012.05.007.
Karalija E., Parić A. Biol. Nyssana, 2011, vol. 2, pp. 29–35.
Danova K., Čellárová E., Macková A., Daxnerová Z., Kapchina-Toteva V. In Vitro Cell Dev. Biol.-Plant, 2010, vol. 46, pp. 422–429. DOI: 10.1007/s11627-010-9299-2.
Santiago L., Louro R. Annals of botany, 2000, vol. 86, pp. 1023–1032. DOI: 10.1006/anbo.2000.1271.
Morozov S.V., Tkacheva N.I., Tkachev A.V. Khimiya Rastitel'nogo Syr'ya, 2018, no. 4, pp. 5–28. DOI: 10.14258/jcprm.2018044003. (in Russ.).
Mironova S.O., Tikhomirova L.I., Bazarnova N.G. Tekhnologii i oborudovaniye khimicheskoy, biotekhnologicheskoy i pishchevoy promyshlennosti. Materialy XI Vserossiyskoy nauchno-prakticheskoy konferentsii studentov, aspirantov i molodykh uchenykh s mezhdunarodnym uchastiyem. [Technologies and equipment of the chemical, biotechnological and food industries. Materials of the XI All-Russian scientific-practical conference of students, graduate students and young scientists with international participation]. Biysk, 2018, pp. 254–257. (in Russ.).
Borisenko N.I. Razvitiye metodologii ispol'zovaniya subkriticheskoy vody dlya polucheniya fiziologicheski aktivnykh substantsiy na osnove rastitel'nykh metabolitov: dis. ... dokt. khim. nauk. [Development of a methodology for the use of subcritical water to obtain physiologically active substances based on plant metabolites: dis. ... doc. Chem. sciences]. Rostov-on-Don, 2014, 284 p. (in Russ.).
Okuda T., Ito H. Molecules, 2011, vol. 16(3), pp. 2191–2217. DOI: 10.3390/molecules16032191.
Hassanpour S., Maheri-Sis N., Eshratkhah B., Mehmandar F.B. Int. J. Forest, Soil and Erosion, 2001, vol. 1(1), pp. 47–53. DOI: 10.1007/s00468-016-1438-x.
Antonova N.P., Kalinin A.M., Prokhvatilova S.S., Shefer Ye.P., Matveyenkova T.Ye. Vedomosti Nauchnogo tsentra ekspertizy sredstv meditsinskogo primeneniya, 2015, no. 1, pp. 11–16. (in Russ.).
Fedoseyeva L.M. Khimiya Rastitel'nogo Syr'ya, 2005, no. 3, pp. 45–50. (in Russ.).
Copyright (c) 2020 chemistry of plant raw material
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.
