Polymorphism in populations of Corydalis subjenisseensis s. l. (Papaveraceae) in the south of the Yenisei Siberia
УДК 582.682:575.174+577.21
Abstract
Corydalis subjenisseensis (Antipova) is a tuberous ephemeroid characterized by a high morphological diversity. During the research work genetic polymorphism of 7 populations Corydalis subjenisseensis s. l., growing in the southof the Yenisei Siberia were analyzed using ISSR markers. The amplification of genomic DNA with 8 ISSR primers yielded100 DNA amplicons of which 78 were polymorphic. The number of amplified DNA fragments, depending on the primer,varied from 9 (ISSR-17) to 21 (HB14). The maximum level of genetic variation was observed for Western Sayan populations growing in aspen and fir forests. The genetic differentiation among populations (Gst) was 0.2415, indicating a highlevel of differentiation. The similarity dendrogram performed in the TFPGA program shows a division into 2 groups: thefirst group includes the Krasnoyarsk and Khakass populations of C. subjenisseensis, the second group includes the Tanzybei populations, which are characterized by a high level of polymorphism. A similar structure is observed when buildingclusters using the Bayesian approach. 69 genotypes are divided into a maximum of 7 genetic clusters. Among the populations of the Tanzybei, individuals of presumably hybridogenic origin are found, grouped around two centers of “attraction”.
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Антипова Е. М. Corydalis subjenisseensis (Antipova) – Хохлатка приенисейская // Красная книга Красноярского края. В 2-х т. Т. 2: Редкие и находящиеся под угрозой исчезновения виды дикорастущих растений и грибов. 2-е изд., перераб. и доп.; Сибирский фед. ун-т. – Красноярск, 2012. – 576 с.
Степанов Н. В. Новые виды сиреневоцветковых хохлаток (Corydalis DC., Fumariaceae) из Приенисейских Саян // Сист. зам. Герб. Томск. ун-та, 2018. – №. 117. – С. 16–34.
Earl D. A., von Holdt B. M. STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method // Conservation Genet Resour, 2012. – № 4. – P. 359–361.
Evanno G., Regnaut S., Goudet J. Detecting the number of clusters of individuals using the software structure: a simulation study // Molecular Ecology, 2005. – № 14. – P. 2611–2620.
Nei M. Genetic Distance between Populations // American Naturalist, 1972. – № 106. – P. 283–292.
Pritchard J. K., Stephens M., Donnelly P. Inference of population structure using multilocus genotype data // Genetics, 2000. – № 155. – P. 945–959.
Zietkiewicz E., Rafalski A., Labuda D. Genome fingerprinting by simple sequence repeat (SSR) anchored polymerase chain reaction amplification // Genomics, 1994. – № 20(2). – P. 176–18
