Obtaining Glycyrrhiza uralensis “hairy” roots: primary assessment of growth characteristics and content of phenolic compounds
УДК 582.739:581.143.6:577.13
Abstract
Widespread use and uncontrolled harvesting of wild plants of Ural licorice (Glycyrrhiza uralensis Fisch. ex DC.) as a raw material for pharmacology and the food industry determines the promise of the system of “hairy” roots for obtaining valuable secondary metabolites. The aim of the research is to develop an effective protocol for the agrobacterial transformation of Rhizobium rhizogenes of various types of explants of 30-day-old G. uralensis microplants: cotyledons, primary shoots, hypocotyls, and primary roots. The effect of 100 цМ acetosyringone, variation of the cocultivation time (2 and 4 h) on the efficiency of the transformation process by two strains of R. rhizogenes 15834 SWISS and MSU 440 was studied. infection (2 h) and competent types of explants for transformation with the strain (cotyledons, primary shoots, primary roots). As a result of primary selection, 12 lines of stably growing BK cultures were selected. PCR analysis showed that all selected lines contain the rolB and rolC genes, while the virC gene is absent. It was determined that 4 lines are characterized by a high growth rate, 3 are characterized by a high total content of phenolic compounds. To the best of our knowledge, this is the first protocol for obtaining a culture of hairy roots from G. uralensis using strains R. rhizogenes 15834 SWISS and MSU 440.
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