On the identity of the species Pulsatilla astragalifolia (Ranunculaceae)
УДК 582.675.1:581.961+575.86
Abstract
Pulsatilla is a genus of early-flowering, ornamental, partly medicinal plants, characterized by a high level of hybridization. In this connection, many of them are rare and endemic to individual territories. Several different nomenclature combinations have been made over the extensive history of the genus revising, which often causes debates between scientists. One of the cases is the taxon Pulsatilla astragalifolia Pobed. To try to solve this issue, ITS fragments of nuclear DNA of three close species (Pulsatilla bungeana C. A. Mey. ex Ledeb., P astragalifolia Pobed., P tenuiloba (Hayek) Juz.) were sequenced. Another group is formed by several clades with samples of P. bungeana and P. astragalifolia, According to the results obtained, the studied samples were divided into several groups. One group consists of two clades: the first - P. tenuiloba samples, the second - P. astragalifolia samples. Which may indicate the presence of several forms of these species. Subsequently, it is planned to study new occurrences of these species, as well as the analysis of the other DNA fragments. However, the findings indicate that Pulsatilla astragalifolia is a separate species, having both morphological and genetic differences from related species.
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Бакин О. В. О роде Pulsatilla Mill. (Ranunculaceae) во флоре Татарстана // Труды Волжско-Камского государственного природного заповедника, 2005. - Вып. 6. - С. 193-198.
Губанов И. А. Конспект флоры внешней Монголии (сосудистые растения) / Под ред. Р. В. Камелина. - М.: «Ва-ланг», 1996. - 136 с.
Куцев М. Г., Уварова О. В., Синицына Т. А. Набор синтетических олигонуклеотидов для амплификации и секвенирования ITS1-5.8s-ITS2 сосудистых растений. - № RU 2 528 063 С1; заявл. 18.02.2013; Опубл. 10.09.2014, Бюл. № 25. - 7 с.
Победимова Е. Г. Растительность центральной части Монгольского Алтая // Труды Монгольской комиссии, 1935. - № 19. - С. 50-52.
Степанов Н. В. Заметки о некоторых видах Pulsatilla L. (Ranunculaceae) из приенисейских Саян // Систематические заметки по материалам гербария им. П. Н. Крылова томского государственного университета, 2014. -№ 109. - С. 6-19.
Aichele D., Schwegleb H. W. Die Taxonomie der Gattung Pulsatilla // Repertorium novarum specierum regni vegetabilis, 1957. - Vol. 60. - P. 1-230. DOI: 10.1002/fedr.19570600103
Akeroyd J. R. Pulsatilla Miller // Flora Europaea / Tutin T. G., Burges N. A., Chater A. O. et al. (eds.). - Cambridge: Cambridge University Press, 1993. - Vol. 1. - P. 264-266.
GBIF [2023]. Global Biodiversity Information Facility. URL: https://www.gbif.org/ (Accessed 30 September 2023).
Grey-Wilson C. Pasque-flowers. The genus Pulsatilla. - Kenninghall: The Charlotte-Louise Press, 2014. - 216 p.
Jinmiao Z., Lihua T., Meiwan C., Chengwei H. Pharmacological activities and molecular mechanisms of Pulsatilla saponins // Chinese Medicine, 2022. - Vol. 17, No. 59. - P. 1-12. DOI: 10.1186/s13020-022-00613-8
Krvavych A., Reviakina N., Zhurakhivska L., Hubytska I., Konechna R. Pulsatilla alba: Analytical Review of Spread, Chemical Composition, Biological Activity and Medical Application // Scientific Journal «ScienceRise: Biological Science», 2021. - Vol. 29. - No. 4. - P. 10-14. DOI: 10.15587/2519-8025.2021.249850
Liu H., Hu Y., Qi B., Yan C., WangL., Zhang Y., Chen L. Network pharmacology and molecular docking to elucidate the mechanism of Pulsatilla decoction in the treatment of colon cancer // Front. Pharmacol, 2022. - Vol. 300. DOI: 10.3389/ fphar.2022.940508
Li Q-j., Wang X., Wang J-r., Su N., Zhang L., Ma Y-p., Chang Z-y., Zhao L., Potter D. Efficient Identification of Pulsatilla (Ranunculaceae) Using DNA Barcodes and Micro-Morphological Characters // Front. Plant Sci., 2019. -Vol. 10. - e1196. DOI: 10.3389/fpls.2019.01196
Sramko G., Laczko L., Volkova P. A., Bateman R. M., Mlinarec J. Evolutionary history of the Pasque-flowers (Pulsatilla, Ranunculaceae): Molecular phylogenetics, systematics and rDNA evolution // Mol. Phylogenet. Evol., 2019. - Vol. 135. -P. 45-61. DOI: 10.1016/j.ympev.2019.02.015
Tamura M. Systematic Part // Die Naturlichen Pflanzenfamilien nebst ihren Gattungen und wichtigsten Arten, inbesondere den Nutzpflanzen. Band 17 a IV. Angiospermae: Ordnung Ranunculales, Fam. Ranunculaceae / P. Hiepko (Ed.). - Berlin: Duncker & Humbolt, 1995. - P. 223-519.
Valuyskikh O. E., Teteryuk L. V., Pylina Y. I., Sushentsov O. E., Martynenko N. A., Shadrin D. M. Phylogenetic relationships and status of taxa of Pulsatilla uralensis and P. patens s. str. (Ranunculaceae) in north-eastern European Russia // PhytoKeys, 2020. - Vol. 162. - P. 113-130. DOI: 10.3897/phytokeys.162.53361
