Development of CAPS-markers for studying plastid loci polymorphism in Rubus L. subgenus Idaeobathus Focke
УДК 582.734.4+575.113+575.174.015.3+634.71(571.150/.151)
Abstract
Genus Rubus L. including subgenus Idaeobathus Focke (raspberry) has difficult structure. Studying this subgenus researchers have special interest to West Siberia and especially Altai region because two species R. idaeus L. and R. sachalinensis H. Lev. grow together on this territory. Usually in phylogenetic research chloroplast DNA loci are studied. This article describes the attempt to develop relevant plastid CAPS-markers for studying raspberry genetic diversity. Nine markers that revealed polymorphism among raspberry cultivars have been developed. However, the sample of R. idaeus and R. sachalinensis herbarium specimens from Altai Krai and Republic of Altai showed that all of them are monomorphic aside from two combinations “primers/restrictase” indicated intraspecies polymorphism within R. sachalinensis. In addition, two potential markers able to differentiate R. idaeus and R. sachalinensis without using restrictases are detected. Further research devoted to creating markers for revealing plastid loci polymorphism will continue.
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