Genotyping of representatives of the genus Artemisia growing on the territory of Yakutia
УДК 582.998.2+575.11(571.56)
Abstract
On the territory of the Republic of Sakha (Yakutia) there are 22 species of the genus Artemisia L. (family Asteraceae). Genetic diversity and intraspecific changes in representatives of the genus Artemisia are considered as a dependence on climatic factors. The aim of this study is to genotype representatives of the genus Artemisia growing in Yakutia using RAPD analysis. The RAPD method allows it to work effectively with previously unstudied species. DNA samples from plant material of 10 wild species of Artemisia growing in Central (Amginsky and Megino-Kangalassky districts), North-Eastern (Oymyakonsky district) and Southern Yakutia (Olekminsky district) and 4 species introduced in the conditions of the Botanical Garden of the North-Eastern Federal University of Yakutia were considered and analyzed. Samples of plant material were collected during stationary-route expeditionary works during 2016-2022. Using RAPD analysis with arbitrary primer, 101 bands, most of which are polymorphic, were detected in the amplification products. A primary dendrogram with distribution into five corresponding clusters was obtained. Data on possible interspecific similarity and polymorphism among the considered wild and introduced species of wormwood were obtained. The results of the study require further evaluation using bioinformatic analysis.
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