Features of seed treatment of rare and endangered species of plants of Yakutia for in vitro cultivation

УДК 631.53.027.32:57.085.23

Abstract

The aim of the study is to develop effective sterilization protocols for seeds of rare and endangered plant species of Yakutia for their introduction into in vitro culture. The objects of the study are Dracocephalum jacutense, Krascheninnikovia lenensis, Phlojodicarpus sibiricus, Lilium pilosiusculum and Artemisia martjanovii. The seeds of the objects of the study were collected during expeditionary work in the regions of Western, Eastern, Central and Southern Yakutia. After drying and treatment, the seeds were stored in a refrigerator. The linear dimensions, the shape and condition of the seed surface were studied. For each plant species, different protocols for surface treatment of seeds were developed and tested. Sodium hypochlorite, ethyl alcohol, hydrogen peroxide, Domestos disinfectant solution, potassium permanganate solution, etc. were used as sterilizing agents. After testing different methods of surface treatment of the seeds of the objects under study, effective protocols for seed preparation and sterilization were selected. Sterile sprouts of rare and endangered plant species of Yakutia were obtained. The sprouts were brought to the development of 4-5 true leaves and used to obtain different explants for introducing the objects of study into in vitro culture.