Genetic diversity of some Astragalus taxa of the section Cenantrum Bunge
УДК 58.006:502.75+57.065(571.53/.54)
Abstract
Issues of genetic variability, phylogenetic relationships, and taxonomic status of representatives of the section Cenantrum Bunge remain relevant, despite the significant number of studies of some species. Astragalus mongholicus Bunge and Astragalus frigidus (L.) A. Gray are characterized by a wide range, flexible morphological characters and the presence of variants and subspecies whose identification is difficult. According to the taxonomic revision of the genus Astragalus (Podlech, Zarre, 2013), the following are synonymized with Astragalus mongholicus Bunge: Astragalus membranaceus Bunge, Astragalus borealimongolicus Y. Z. Zhao, Astragalus propinquus Schischk., Astragalus mongholicus var. dahuricus (DC.) Podlech and others. However, this taxonomy does not always agree with the results of molecular data. The current study is aimed at molecular identification, determination of genetic diversity, and evolutionary relationship of A. mongholicus, A. membranaceus, A. propinquus, A. frigidus, and Astragalus saralensis Gontsch., for which we analyzed the molecular marker ITS1-ITS2 in 18 samples from the herbarium of the SIFIBR SB RAS. For comparison, ITS nucleotide sequences from the GenBank database were used. Sequence analysis and haplotype network construction showed that A. mongholicus (including A. membranaceus), A. propinquus (including A. borealimongolicus) are genetically distinct taxa. At the same time, A. frigidus and A. saralensis are congeneric, which requires further analysis of systematic position of A. saralensis. It was shown that when using the ITS marker, no genetic differences were found between Astragalus frigidus (L.) A. Gray subsp. secundus (DC.) Vorosch. and Astragalus frigidus subsp. parviflorus Hulten. It is confirmed that A. mongholicus var. dahuricus is not closely related to A. mongholicus and other species of the section Cenantrum. Based on ITS, A. propinquus and A. borealimongolicus are genetically identical. The values of intra- and interspecific genetic variability for the studied taxa are provided.
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References
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