Molecular genetic analysis of Lamiaceae species in Uzbekistan based on DNA markers

УДК 582.949.2+502.3+575.113.1(575.1)

Abstract

Rare and endangered plant species are particularly sensitive to climate change and destructive anthropogenic impacts. The latest edition of the Red Book of Uzbekistan includes 314 plant species, which is about 7 % of the flora of Uzbekistan. 30 species of the Lamiaceae family are included in the Red Book. Rare and endangered plant species respond especially sensitively to destructive anthropogenic impacts and climate change. This work is devoted to the study of the species diversity of rare species of the Lamiaceae family in the flora of Uzbekistan using combinations of DNA markers. Plastid and nuclear regions (ITS, rbcL, trnL-trnF, matK) were analyzed as DNA markers in some species of Lamiaceae. Species identification using DNA markers is also shown. The level of polymorphism trnL-trnF region within Lagochilus is assessed. A phylogenetic analysis was carried out based on the marker sequences encoding the ITS+trnL-trnF multilocus for representatives of the family Lamiaceae. The division of the Lamiaceae family into two clusters, Lamioideae + Scutellarioideae and Nepetoideae, was confirmed. The distribution of species within the clusters was consistent with the ecological and morphological classification of the family. Polymorphism of the chloroplast region trnL-trnF on seven representatives of the Lagochilus genus allowed us to differentiate closely related species of this genus. The identification of the rare species Lagochilus olgae was confirmed based on the four DNA loci.