Use of the internal transcribed spacer (ITS) and translation elongation factor (tef1a) to identify Trichoderma species
UDC 575.2
Abstract
Trichoderma species are of great practical importance, producing enzymes (cellulases, xylanases), antibiotics and plant growth stimulants. Genetic studies help to select new strains and isolates for practical use. Molecular genetic studies of Trichoderma species are becoming increasingly important due to their biotechnological potential, ecological significance and difficulties of traditional identification. Trichoderma species have high morphological plasticity, which complicates their differentiation by microscopic and cultural features. The ITS (internal transcribed spacer) fragment is standard for species identification, but for Trichoderma it is often insufficient due to high conservatism. Some species have identical ITS sequences, which require additional genetic markers. The aim of the work is to compare the ITS fragment and the translation elongation factor (tef1a) for identifying Trichoderma species. As a result of the analysis, the species identity was confirmed with high accuracy. ITS and tef1a markers can be used both individually and jointly to identify species of the genus, contamination control in collections, and purity of mother cultures in biotechnological industries.
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References
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