Use of fruits with different storage periods in the study of ploidy and DNA content of Peucedanum vaginatum Ledeb. (Umbelliferae Juss.) by flow cytometry
UDC 576.3:582.893
Abstract
In this paper, using Peucedanum vaginatum as an example, we consider methodological issues related to the possibility of using Umbelliferae fruits of different storage periods to determine the genome size and ploidy levels by flow cytometry. The structural features of the fruits determine the configuration of the fluorescence signal, when the predominant peak in the histograms corresponds to the triploid endosperm, which occupies most of the seed, and the weak peak corresponds to the diploid small embryo. The seed coat and the pericarp fused with it, being dry and consisting of dead cells, do not participate in the formation of the signal. As the storage period increases, the number of registered events decreases, the embryo peak disappears, and deviations in the values of the genome size increase up to 27 %, therefore, information on the DNA content is only approximate and can be used to estimate the ploidy level. Nevertheless, the possibility of using herbarium material with mature fruits, the storage periods of which can exceed 100 years, provides karyological studies with a wide sample and large-scale geographic coverage.
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