EXPRESSION OF THE ALKB AND MMOAA GENES IN RHODOCOCCUS CELLS IN THE PRESENCE OF C3–C16 N-ALKANES
PDF (Русский)

Keywords

actinomycetes of the genus Rhodococcus
n-alkanes
biodegradation
gene expression
alkane-1-monooxygenases AlkB
propane monooxygenase MmoA

How to Cite

Komarova L., Krivoruchko A., Ivshina I. EXPRESSION OF THE ALKB AND MMOAA GENES IN RHODOCOCCUS CELLS IN THE PRESENCE OF C3–C16 N-ALKANES // BIOAsia-Altai, 2024. Vol. 4, № 1. P. 108-112. URL: http://journal.asu.ru/bioasia/article/view/16140.

Abstract

In the presence of n-alkanes C3–C4 and C9–C16 in collection strains of Rhodococcus cells, the expression level of alkB genes encoding the synthesis of alkan-1-monooxygenases increases in 30000–3000000 times. Additionally in R. ruber cells in the presence of n-alkanes C3–C4, the expression level of mmoAa gene encoding the synthesis of α-subunit of propanmonooxygenase increases in 26000–52000 times. In the presence of toxic n-alkanes C5–C8, the expression of alkB and mmoAa genes does not change compared to the control (LB medium).

PDF (Русский)

References

Патрушев Л.И. Экспрессия генов. Москва. 2000. 526 с.

Fernandes P.J., Powell J.A.C., Archer J.A.C. Construction of Rhodococcus random mutagenesis libraries using Tn5 transposition complexes // Microbiology Society. 2001. V. 147. No. 9. P. 2529–2536.

Ivshina I.B., Kuyukina M.S., Krivoruchko A.V. Hydrocarbonoxidizing bacteria and their potential in eco-biotechnology and bioremediation. In: Microbial Resources: From Functional Existence in Nature to Industrial Applications // Ed. Kurtböke I. Elsevier, Amsterdam. 2017. P. 121–148.

Koch D.J., Chen M.M., van Beilen J.B., Arnold F.H. In vivo evolution of butane oxidation by terminal alkane hydroxylases AlkB and CYP153A6 // Applied and Environmental Microbiology. 2009. V. 75. No. 2. P. 337–344.

Kuyukina M.S., Ivshina I.B. Production of trehalolipid biosurfactants by Rhodococcus. Biology of Rhodococcus, 2nd edition // Ed. H. Alvarez. Springer Nature. 2019. P. 271–298.

Táncsics A., Benedek T., Szoboszlay S., Veres P.G., Farkas M., Mathe I., Marialigeti K., Kukolya J., Lanyi S., Kriszt B. The detection and phylogenetic analysis of the alkane 1-monooxygenase gene of members of the genus Rhodococcus // Systematic and Applied Microbiology. 2015. V. 38. P. 1‒7.

Altai State University is a golden publisher, as we allow self-archiving, but most importantly we are fully transparent about your rights.

Authors may present and discuss their findings ahead of publication: at biological or scientific conferences, on preprint servers, in public databases, and in blogs, wikis, tweets, and other informal communication channels.

We allows authors to deposit manuscripts (currently under review or those for intended submission to ABS) in non-commercial, pre-print servers such as ArXiv.

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY 4.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
    2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
    3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).

Metrics

Metrics Loading ...