Investigation of the genus Anemone s. l. (Ranunculaceae) phylogeny using ITS and matK molecular markers
УДК 582.675.1:575
Abstract
чThe phylogenetic analysis of Siberian representatives of genus Anemone s. l. were performed for the first time, based on a comparative study of nucleotide polymorphism of ITS (Internal Transcribed Spacer) region of the nuclear genome and matK (Maturase K) gene of the chloroplast genome. 54 DNA sequences (27 for each marker) and 8 DNA sequences from GenBank were used for analysis. Two phylogenetic trees were built, each of which is combined for the maximum likelihood (ML) and Bayesian inference (BI) methods. Topologies derived from two separate datasets are mostly consistent with each other. However, there are differences in the location of individual species (A. sylvestris, A. osinovskiensis). On two constructed trees, the sequences clearly split into two well-supported clades: the first is formed by anemone with karyotype x = 8, the second with x = 7. Our results are consistent with the data of other authors who have developed the taxonomy of the genus Anemone both on the basis of morphological features and using molecular markers. Individuals of the same species, growing in geographically distant parts of the range, either do not differ from each other in the studied DNA sequences, or have 1-3 point mutations.
Downloads
Metrics
References
Куцев М. Г., Уварова О. В., Синицина Т. А. Набор синтетических олигонуклеотидов для амплификации и секвенирования ITS1-5.8S-ITS2 сосудистых растений / Патент на изобретение RU 2528063 C1, 10.09.2014. Заявка № 2013106976/10 от 18.02.2013
Луферов А. Н. Таксономический конспект лютиковых (Ranunculaceae) Дальнего Востока России // Turczaninowia, 2004. - Т. 7, № 1. - С. 5-84.
Стародубцев В. Н. Ветреницы: систематика и эволюция. - Л.: Наука, 1990. - 200 с.
Степанов Н. В. Флора северо-востока Западного Саяна и острова Отдыха на Енисее (г. Красноярск). - Красноярск: Изд-во Краснояр. гос. ун-та, 2006. - 122 с.
Ehrendorfer F., Samuel R. Contributions to a molecular phylogeny and systematics of Anemone and related genera (Ranunculaceae-Anemoninae) // Acta Phytotax. Sin., 2001. - Vol. 39. - P. 293-307.
Ehrendorfer F., Ziman S. N., Konig C., Keener C. S., Dutton B. E., et al. Taxonomic revision, phylogenetics and transcontinental distribution of Anemone section Anemone (Ranunculaceae) // Bot. J. Linn. Soc., 2009. - Vol. 160. -P. 312-354. DOI: 10.1111/j.1095-8339.2009.00861
Gouy M., Guindon S., Gascuel O. SeaView version 4: a multiplatform graphical user interface for sequence alignment and phylogenetic tree building // Molecular Biology and Evolution, 2010. - Vol. 27, No 2. - P. 221-224. URL: http://doua. prabi.fr/software/seaview
Hollingsworth M. L., Clark A. A., Forrest L. L., et al. Selecting barcoding loci for plants: evaluation of seven candidate loci with species-level sampling in three divergent groups of land plants // Molecular Ecology Resources, 2009. - Vol. 9. -P. 439-457.
Hoot S. B., Meyer K. M., Manning J. C. Phylogeny and reclassification of Anemone (Ranunculaceae), with an emphasis on austral species // Systematic Botany, 2012. - Vol. 37. - P. 139-152. DOI: 10.1600/036364412X616729
Hoot S. B., Reznicek A. A, Palmer J. D. Phylogenetic relationships in Anemone (Ranuculaceae) based on morphology and chloroplast DNA // Systematic Botany, 1994. - Vol. 19, No 1. - P. 169-200. DOI: 10.2307/2419720
JiangN., Zhou Z., Yang J-B., Zhang S-D., Guan K-Y., Tan Y-H., et al. Phylogenetic reassessment of tribe Anemoneae (Ranunculaceae): Non-monophyly of Anemone s. l. revealed by plastid datasets // PLoS ONE, 2017. - Vol. 12, No 3. DOI: 10.1371/journal.pone.0174792
Meyer K. M., Hoot S. B., Arroyo M. T. K. Phylogenetic affinities of south American Anemone (Ranunculaceae), including the endemic segregate genera, Barneoudia and Oreithale // Int. J. Plant Sci., 2010. - Vol. 171. - P. 323-331. DOI: 10.1086/650153
Minh B. Q., Schmidt H. A., Chernomor O., Schrempf D., WoodhamsM. D., Haeseler A. von, Lanfear R. IQ-TREE 2: New models and efficient methods for phylogenetic inference in the genomic era // Mol. Biol. Evol., 2020. - Vol. 37. -P. 1530-1534. URL: http://www.iqtree.org
Mlinarec J., Satovic Z., Mihelj D., Malenica N., Besendorfer V. Cytogenetic and phylogenetic studies of diploid and polyploid members of tribe Anemoninae (Ranunculaceae) // Plant Biol., 2012. - Vol. 14. - P. 525-536. DOI: 10.1111/ j.1438-8677.2011.00519
Ronquist F., Huelsenbeck J. P. MRBAYES 3: Bayesian phylogenetic inference under mixed models // Bioinformatics, 2003. - Vol. 19. - P. 1572-1574. URL: http://nbisweden.github.io/MrBayes/manual.html
Schuettpelz E., Hoot S. B., Samuel R., Ehrendorfer F. Multiple origins of southern hemisphere Anemone (Ranunculaceae) based on plastid and nuclear sequence data // Plant Syst. Evol., 2002. - Vol. 231. - P. 143-151. DOI: 10.1007/s006060200016
Stover B. C., Muller K. F. TreeGraph 2: Combining and visualizing evidence from different phylogenetic analyses // BMC Bioinformatics, 2010. - Vol. 11, No 7. URL: http://treegraph.bioinfweb.info
Tamura M. Anemone L. // Hiepko P. (ed.), Die Naturlichen Pflanzenfamilien, 1995. - Vol. 17a. - P. 324-349.
Zhang Y., Hong Y., Ren C., TangM., Hoot S. B., Yang Q. Palynology, cytology, and molecular systematics of Anemone section Begoniifolia (Ranunculaceae) // Plant Syst. Evol., 2015. - Vol. 301. - P. 411-424. DOI: 10.1007/s00606-014-1082-0
Ziman S. N., Bulakh E. V., Kadota Y., Keener C. S. Modern view on the taxonomy of the genus Anemone L. sensu stricto (Ranunculaceae) // J. Jpn. Bot., 2008. - Vol. 83. - P. 127-155.
