EVALUATION OF THE POSSIBILITY OF USING MOLECULAR MARKERS TO IDENTIFY GENOTYPES OF HYBRID FORMS OF RHODODENDRONS DURING IN VIVO AND IN VITRO CULTIVATION
Abstract
Formation of living collections of plants in vivo and in vitro makes it possible to preserve valuable genotypes, multiply plants, conduct a whole range of fundamental and applied research, and obtain healthy material for use in breeding work. Creation of such collections necessitates studies confirming the identity of the original specimens and regenerants obtained in aseptic culture. Such studies are of particular importance for species that are easily crossed with each other to form hybrid forms. The work shows the possibility and expediency of using nuclear and chloroplast molecular markers to identify genotypes of hybrid forms of rhododendrons during their in vivo and in vitro cultivation, with a sufficiently high degree of reliability of the results obtained, which is the most important condition for depositing plants in living collections.
References
Васильева О.Г. Биолого-морфологические основы клонального микроразмножения некоторых представителей рода Rododendron L. Автореферат дисс. канд. биол. наук. М.: ГБС РАН, 2009, 22 с.
Криницына А.А., Меркушкин Д.С., Чурикова О.А. Возможность использования системы SRAP для молекулярной паспортизации сортов сирени в сборнике Всероссийской конференции «Коллекции как основа изучения генетических ресурсов растений и грибов» (в рамках первого научного форума «Генетические ресурсы России», 21–24 июня 2022 г.). Ботанический институт им. В.Л. Комарова РАН, г. Санкт-Петербург, тезисы, с. 26.
Молканова О.И., Королева О.В., Стахеева Т.С., Крахмалева И.Л., Мелещук Е.А. Совершенствование технологии клонального микроразмножения ценных плодовых и ягодных культур для производственных условий. Достижения науки и техники АПК. – 2018. – Т.32. –№ 9. – С. 66 – 69.
Anderson W.C. A revised tissue cultured medium for shoot multiplication of rhododendron. J. Am. Soc. Hortic. Sсi. 1984. – Vol. 109. – P. 343-347.
Chamberlain D.F. A revision of Rhododendron II: subgenus Hyemanthes. Notes from the Royal Botanic Garden, Edinburgh. – 1982. – Vol. 39. - P. 209 – 486.
Peakall R., Smouse P.E. GENALEX 6: Genetic Analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes. – 2006. – Vol. 6. – P. 288-295.
Zhang J.L., Zhang C.Q., Gao L.M. et al. Natural hybridization origin of Rhododendron agastum (Ericaceae) in Yunnan, China: inferred from morphological and molecular evidence. J Plant Res. – 2007. – Vol. 120. – P.457-463
Copyright (c) 2024 O.G. Vasilieva, A.A. Krinitsyna, O.A. Churikova

This work is licensed under a Creative Commons Attribution 4.0 International License.
Altai State University is a golden publisher, as we allow self-archiving, but most importantly we are fully transparent about your rights.
Authors may present and discuss their findings ahead of publication: at biological or scientific conferences, on preprint servers, in public databases, and in blogs, wikis, tweets, and other informal communication channels.
We allows authors to deposit manuscripts (currently under review or those for intended submission to ABS) in non-commercial, pre-print servers such as ArXiv.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY 4.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
