Abstract
Fats of different animal origin were studied as the only carbon substrate for PGA synthesis by the natural strain Cupriavidus necator B-10646. The studied fatty substrates differed in qualitative and quantitative fatty acid composition and were represented from 7 to 23 fatty acids with palmitic, stearic and oleic acids dominating and saturation ratios of 0.2-1.7. All investigated C-substrates supported the producer growth and PGA synthesis: bacterial biomass concentration and intracellular polymer content were 1.5-6.5 g/L and 51-70%, respectively. The synthesized PGAs were three-component copolymers with a predominance of 3-hydroxybutyrate monomers (94.9-96.6 mol%) and minor inclusions of 3-hydroxyvalerate (3.0-4.6 mol%) and 3-hydroxyhexanoate (0.4-0.6 mol%).
References
Abrha H., Cabrera J. et al. Bio-based plastics production, impact and end of life: A literature review and content analysis // Sustainability. – 2022. – Vol. 14. – №. 8. – P. 4855.
Akiyama M., Tsuge T. et al. Environmental life cycle comparison of polyhydroxyalkanoates produced from renewable carbon resources by bacterial fermentation // Polym Degrad Stab. – 2003. – Vol. 80. – №. 1. – P. 183-194.
Anderson A.J., Dawes E.A. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates // Microbiol Rev. – 1990. – Vol. 54. – №. 4. – P. 450-472.
Braunegg G., Sonnleitner B.Y. et al. A rapid gas chromatographic method for the determination of poly-β-hydroxybutyric acid in microbial biomass // Eur J Appl Microbiol Biotechnol. – 1978. – Vol. 6. – P. 29-37.
Brigham C.J., Budde C.F. et al. Elucidation of β-oxidation pathways in Ralstonia eutropha H16 by examination of global gene expression // J Bacteriol. – 2010. – Vol. 192. – №. 20. – P. 5454-5464.
da Cruz Pradella J.G. Economics and industrial aspects of PHA production // The Handbook of Polyhydroxyalkanoates. – 2020. – P. 389-404.
Favaro L., Basaglia M. et al. Bacterial production of PHAs from lipid-rich by-products // Applied Food Biotechnology. – 2019. – Vol. 6. – №. 1. – P. 45-52.
Gutschmann B., Simões M.M. et al. Continuous feeding strategy for polyhydroxyalkanoate production from solid waste animal fat at laboratory‐and pilot‐scale // Microbial Biotechnol. – 2023. – Vol. 16. – №. 2. – P. 295-306.
Maddikeri G.L., Pandit A.B. et al. Intensification approaches for biodiesel synthesis from waste cooking oil: a review // Ind Eng Chem Res. – 2012. – Vol. 51. – №. 45. – P. 14610-14628.
Obruca S., Sedlacek P. et al. Novel unexpected functions of PHA granules // Appl Microbiol Biotechnol. – 2020. – Vol. 104. – №. 11. – P. 4795-4810.
Riedel S.L., Jahns S. et al. Polyhydroxyalkanoates production with Ralstonia eutropha from low quality waste animal fats // J Biotechnol. – 2015. – Vol. 214. – P. 119-127.
Zhila N.O., Sapozhnikova K.Y. et al. Synthesis and properties of polyhydroxyalkanoates on waste fish oil from the production of canned sprats // Processes. – 2023. – Vol. 11. – № 7. – P. 2113.

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