Keywords
alkaline delignification
enzymatic hydrolysis
biosynthesis
bacterial nanocellulose
How to Cite
Abstract
Bacterial nanocellulose is a unique material that has no analogues and has a huge range of applications. In this work, alkaline delignification with 4% sodium hydroxide solution was chosen as a chemical pretreatment, and the alkaline delignification product of miscanthus was obtained as a result. As a result of this treatment, the chemical composition of the alkaline delignification product changed in comparison with native miscanthus as follows: decrease in lignin content by 2.3 times and pentosans by 9.0 times, increase in cellulose content by 1.7 times. As a result of enzymatic hydrolysis of alkaline delignification product of miscanthus, the final concentration of reducing substances reached 12.0 g/L, nutrient medium was prepared from this hydrolysate (by introducing phenolic substances of black tea and pasteurization), then bacterial nanocellulose biosynthesis was carried out. The symbiotic culture of Medusomyces gisevii Sa-12 was used as the producer. As a control, biosynthesis of bacterial nanocellulose was carried out on semi-synthetic glucose nutrient medium under the same conditions. It was found that on the medium obtained from miscanthus, the number of acetic acid bacteria was 4.0 times lower, the number of yeasts was 2.5 times lower, the yield of bacterial nanocellulose was 3.8 times lower, and the degree of polymerization of bacterial nanocellulose was 2.9 times lower than on the semi-synthetic nutrient medium. These indicators indicate the biological poor quality of the nutrient medium obtained from miscanthus, which is primarily due to the method of pre-treatment (alkaline delignification) and is presumably associated with the presence of residual sodium ions in the nutrient medium. It should be emphasized that the results of scanning electron microscopy revealed that the thickness of microfibrils of bacterial nanocellulose obtained on miscanthus medium was 1.5 times lower than in the control.
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