Keywords
Xanthomonas campestris
UDP-glucose:undecaprenyl-phosphate glucose-1-phosphate transferase enzyme
cultivation
How to Cite
Abstract
The extracellular heteropolysaccharide xanthan synthesized by bacteria of the genus Xanthomonas is widely used as a thickener and stabilizer in the food, cosmetic and pharmaceutical industries, and in the oil industry. The in silico study of bacterial enzymes involved in the production of the target biotechnological product is an actual direction of research. The solution of this problem is the use of specialized computer programs in the Internet, such as protein sequence database UniProt, complex information system on enzymes BRENDA. Analysis of databases showed that the enzyme UDP-glucose:undecaprenyl-phosphate glucose-1-phosphate transferase initiates xanthan biosynthesis by bacteria Xanthomonas campestris. The conditions of cultivation in Biosan bioreactor of Xanthomonas campestris bacteria, provided from the Departmental collection of cultures of useful microorganisms for agricultural purposes of the All-Russian Research Institute of Agricultural Microbiology of Rosselkhozakademy, were selected. It can be assumed that the increase in the activity of xanthan biosynthesis initiation enzyme by Xanthomonas campestris bacteria will begin after 65 hours from the beginning of bacteria cultivation on yeast nutrient medium, at a temperature of 30°C, after the transition of the microbial growth curve to the stationary phase.
References
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