Keywords
bioconversion
pharmpollutants
meloxicam
Gordonia
metabolites
How to Cite
Abstract
Non-steroidal anti-inflammatory drugs (NSAIDs) are extensively used in natural habitats as a result of ongoing use, which negatively affects living things at many levels, from the molecular to the ecological. Stress-tolerant actinomycetes (class Actinomycetes) are increasingly relevant to address the issue of pharmaceutical environmental pollution as interest in biotechnology solutions in the global bioeconomy grows. We chose the strain of Gordonia alkanivorans IEGM 1277, which is able to bioconvert meloxicam, one of the most often found NSAIDs in open ecosystems, to hydroxymethyl- and carboxymeloxicam in wastewater from pharmaceutical manufacturing. Meloxicam metabolites have not been shown to be harmful to test bacterial cultures or vertebrates in experiments. It was determined that gordonium cells undergo marked adaptive changes using extremely sensitive micro- and spectroscopy techniques (AFM, TEM-EDX). These changes ranged from the synthesis of biosurfactants and modification of morphometric properties (length, width, volume, etc.) to the accumulation of intracellular lipids and polyphosphates. The actinomycete G. alkanivorans IEGM 1277 underwent whole genome sequencing in order to provide a Catalog of genes encoding meloxicam oxidation enzymes.
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