Keywords
somatic embryogenesis
in vitro cluture
phytohormones
embryos
common pine
forest seed plantation
Karelia
in vitro cluture
phytohormones
embryos
common pine
forest seed plantation
Karelia
How to Cite
Ignatenko R., Yershova M., Chirva O., Galibina N. OBTAINING THE REGENERANT PLANTS OF PINUS SYLVESTRIS L. PLUS CLONES // BIOAsia-Altai, 2024. Vol. 4, № 1. P. 303-307. URL: http://journal.asu.ru/bioasia/article/view/16350.
Abstract
Donor plants of Pinus sylvestris explants from Petrozavodsk forest seed plantation capable of somatic embryogenesis initiation were studied, and the formation of embryogenic cell lines on MSG nutrient media with different content and sources of phytohormones was evaluated. The initiation of somatic embryogenesis was observed in explants selected from 2 out of 9 studied clones of plus trees. It was found that embryogenic cell masses were formed with low frequency on all substrates studied. The initial stages of somatic embryo maturation were recorded after seven days of incubation of cell masses on hormone-free medium and then on substrate with ABA. At the end of the experiment, regenerant plants of Pinus sylvestris could not be obtained.
References
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3. Раевский Б.В., Игнатенко Р.В., Новичонок Е.В., Прокопюк В.М., Куклина К.К. Современное состояние селекции и семеноводства хвойных пород // Известия вузов. Лесной журнал. 2022. № 6. С. 9–37. https://doi.org/10.37482/0536-1036-2022-6-9-37
4. Шуклина А.С., Третьякова И.Н. Соматический эмбриогенез видов рода Pinus в культуре in vitro // Успехи современной биологии. 2019. Т. 139, № 2. С. 184–195. https://doi.org/10.1134/S004213241902008X
5. Aronen T., Pehkonen T., Ryynänen L. Enhancement of somatic embryogenesis from immature zygotic embryos of Pinus sylvestris // Scandinavian Journal of Forest Research. 2009. Vol. 24, № 5. P. 372–383. https://doi.org/10.1080/02827580903228862
6. Chirva O.V., Ignatenko R.V., Ershova M.A. Pinus sylvestris L. mature seeds megagamethophytes cultured in vitro. Influence of the genotype and the growth regulators content in nutrient medium on the initiation of callus formation // Plant Cell, Tissue and Organ Culture. 2023. Vol. 152, № 2. P. 299–308. https://doi.org/10.1007/s11240-022-02404-3
7. Galibina N.A., Ershova M.A., Ignatenko R.V., Nikerova K.M., Sofronova I.N., Borodina M.N. Cytogenetic and biochemical characteristics of callus Pinus sylvestris L. // Russian Journal of Plant Physiology. 2023. Vol. 70, № 1. P. 10. http://doi.org.10.1134/S1021443722602348
8. Harju A., Heiska S., Julkunen-Tiitto R., Venäläinen M., Aronen T. Somatic embryogenesis of Pinus sylvestris L. from parent genotypes with high- and low stilbene content in their heartwood // Forests. 2022. Vol. 13, № 4. P. 557. https://doi.org/10.3390/f13040557
9. Ignatenko R.V., Chirva O.V., Ershova M.A., Galibina N.A., Teslyuk I.A. Assessing the ability of Picea abies (L.) H. Karst. plus tree clones from the middle taiga subzone of Karelia to somatic embryogenesis // Russian Journal of Plant Physiology. 2024. Vol. 71, № 25. P. 25. https://doi.org/10.1134/S1021443724604531
10. Keinonen‐Mettälä K., Jalonen P., Eurola P., von Arnold S., von Weissenberg K. Somatic embryogenesis of Pinus sylvestris // Scandinavian Journal of Forest Research. 1996. Vol. 11, № 1-4. P. 242–250. https://doi.org/10.1080/02827589609382933
11. Malabadi R.B., van Staden J. Somatic embryogenesis from vegetative shoot apices of mature trees of Pinus patula // Tree Physiology. 2005. Vol. 25, №. 1. P. 11–16. https://doi.org/10.1093/treephys/25.1.11
12. Niskanen A.M., Lu J., Seitz S., Keinonen K., von Weissenberg K., Pappinen A. Effect of parent genotype on somatic embryogenesis in Scots pine (Pinus sylvestris) // Tree Physiology. 2004. Vol. 24, № 11. P. 1259–1265. https://doi.org/10.1093/treephys/24.11.1259
13. Tret’yakova, I.N., Shuvaev D.N. Somatic embryogenesis in Pinus pumila and productivity of embryogenic lines during long-term cultivation in vitro // Russian Journal of Developmental Biology. 2015. Vol. 46, № 5. P. 276–285. https://doi.org/10.1134/S1062360415050070
14. Tret’yakova I.N., Park M.E. Collectible cell lines of Larix sibirica obtained by somatic embryogenesis and their ability to regenerate // Forests. 2023. Vol. 14, № 9. P. 1920. https://doi.org/10.3390/f14091920
15. Von Arnold S., Sabala I., Bozhkov P., Dyachok J., Filonova L. Developmental pathways of somatic embryogenesis // Plant Cell, Tissue and Organ Culture. 2002. Vol. 69, № 3. P. 233–249. https://doi.org/10.1023/A:1015673200621
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