Keywords
DH-technology
doubled haploids
n vitro microspore culture
cabbage cultures
androgenesis
induction of embryogenesis
embryogenesis factors
doubled haploids
n vitro microspore culture
cabbage cultures
androgenesis
induction of embryogenesis
embryogenesis factors
How to Cite
Mineikina A. FACTORS OF EFFECTIVE EMBRYOGENESIS OF GENOTYPES OF THE GENUS BRASSICA IN ISOLATED MICROSPORES IN VITRO CULTURE // BIOAsia-Altai, 2024. Vol. 4, № 1. P. 335-338. URL: http://journal.asu.ru/bioasia/article/view/16357.
Abstract
Doubled haploid (DH) technology has become a powerful tool for basic and applied plant research. The main and routine use of DH technology is the production of pure lines in the breeding process to obtain hybrids. The success of such biotechnological approaches lies in the high level of efficiency of in vitro plant cell and tissue culture protocols . The processes of induction of embryogenesis and regeneration of plants from embryoids are fundamental for in vitro culture technology of isolated microspores. As a result, we have studied and modified the main factors affecting these processes to increase the embryoid yield in the studied genotypes of plants of the genus Brassica.
References
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15. Winarto B., Teixeira da Silva J.A. Microspore culture protocol for Indonesian Brassica oleracea. Plant Cell and Tissue Organ Culture. 2011, 107, 305-315.
16. Yuan S.X., Su Y.B., Liu Y.M., Fang Z.Y., Yang L.M., Zhuang M., Zhang Y.Y., Sun P.T. Effects of pH, MES, arabinogalactan proteins on microspore cultures in white cabbage. Plant Cell and Tissue Organ Culture. 2012, 110, 69-76.
17. Zhang W., Qiang F., Xigang D., Manzhu B. The culture of isolated microspores of ornamental kale (Brassica oleracea var. acephala) and the importance of genotype to embryo regeneration. Scientia Horticulturae. 2008, 117(1), 69-72.
18. Zou J., Zou, X., Gong Z., Song G., Ren J., Feng H. Thidiazuron Promoted Microspore Embryogenesis and Plant Regeneration in Curly Kale (Brassica oleracea L. convar. acephala var. sabellica). Horticulturae. 2023, 9, 327.
2. Шмыкова Н.А., Шумилина Д.В., Супрунова Т.П. Получение удвоенных гаплоидов у видов рода Brassica L. Вавиловский журнал генетики и селекции. 2015, 19(1), 111-120.
3. Baillie A.M.R., Epp D.J., Hucheson D., Keller W.A. In vitro culture of isolated microspores and regeneration of plants in Brassica campestris. Plant Cell Rep. 1992, 11, 234-237.
4. Bhatia R., Dey S.S., Sood Sh., Sharma K., Chander P., Kumar R. Efficient microspore embryogenesis in cauliflower (Brassica oleracea var. botrytis L.) for development of plants with different ploidy level and their use in breeding programme. Scientia Horticulturae. 2017, 216(3), 83-92.
5. Bhowmik P., Dirpaul, J., Polowick P., Ferrie A.M.R. A high throughput Brassica napus microspore culture system: Influence of percoll gradient separation and bud selection on embryogenesis. Plant Cell Tissue Organ Cult. 2011, 106, 359–362
6. Devaux P., Pickering R. Haploids in the improvement of Poaceae. In C.E. Palmer et al. (ed.); Haploids in crop improvement II. Springer-Verlag: Germany, Berlin, 2005, 215–242.
7. Duijs J.G., Voorrips R.E., Visser D.L., Custers, J.B.M. Microspore culture is successful in most crop types of Brassica oleracea L. Euphytica, 1992, 60, 45-55.
8. Dunwell J.M. Haploids in flowering plants: origins and exploitation. J. Plant Biotech, 2010, 8, 377-424.
9. Lichter R. Induction of haploid plants from isolated pollens of Brassica napus. Z. Pflanzenphysiol. 1982, 105, 427–434.
10. Lichter R. Efficient yield of embryoids by culture of isolated microspores of different Brassicaceae species. Plant Breed. 1989, 103, 119-123.
11. Mineykina A.I, Bondareva L.L, Soldatenko A.V, Domblides E.A. Androgenesis of red cabbage in isolated microspore culture in vitro. Plants. 2021, 10, 1950.
12. Murashige T., Skoog F. Revised medium for rapid growth and bioassays with tobacco tissue. Physiol. Plant 1962, 15, 473–497.
13. Pechan P.M., Keller W.A. Identification of potentially embryogenic microspores in Brassica napus. Physiol. Plant., 1988, 74, 377-384.
14. Simmonds D.H., Keller W.A. Significance of pre-prophase bands of microtubules in the induction of microspore embryogenesis of Brassica napus. Planta. 1999, 208, 383–391
15. Winarto B., Teixeira da Silva J.A. Microspore culture protocol for Indonesian Brassica oleracea. Plant Cell and Tissue Organ Culture. 2011, 107, 305-315.
16. Yuan S.X., Su Y.B., Liu Y.M., Fang Z.Y., Yang L.M., Zhuang M., Zhang Y.Y., Sun P.T. Effects of pH, MES, arabinogalactan proteins on microspore cultures in white cabbage. Plant Cell and Tissue Organ Culture. 2012, 110, 69-76.
17. Zhang W., Qiang F., Xigang D., Manzhu B. The culture of isolated microspores of ornamental kale (Brassica oleracea var. acephala) and the importance of genotype to embryo regeneration. Scientia Horticulturae. 2008, 117(1), 69-72.
18. Zou J., Zou, X., Gong Z., Song G., Ren J., Feng H. Thidiazuron Promoted Microspore Embryogenesis and Plant Regeneration in Curly Kale (Brassica oleracea L. convar. acephala var. sabellica). Horticulturae. 2023, 9, 327.
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