EXPRESSION OF THE ALKB AND MMOAA GENES IN RHODOCOCCUS CELLS IN THE PRESENCE OF C3–C16 N-ALKANES

  • L.P. Komarova Perm State University; Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Centre Email: dulika5365@gmail.com
  • A.V. Krivoruchko Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Centre Email: nast@iegm.ru
  • I.B. Ivshina Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Centre Email: ivshina@iegm.ru
Keywords: actinomycetes of the genus Rhodococcus, n-alkanes, biodegradation, gene expression, alkane-1-monooxygenases AlkB, propane monooxygenase MmoA

Abstract

In the presence of n-alkanes C3–C4 and C9–C16 in collection strains of Rhodococcus cells, the expression level of alkB genes encoding the synthesis of alkan-1-monooxygenases increases in 30000–3000000 times. Additionally in R. ruber cells in the presence of n-alkanes C3–C4, the expression level of mmoAa gene encoding the synthesis of α-subunit of propanmonooxygenase increases in 26000–52000 times. In the presence of toxic n-alkanes C5–C8, the expression of alkB and mmoAa genes does not change compared to the control (LB medium).

Author Biographies

L.P. Komarova, Perm State University; Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Centre

Postgraduate student of Perm State National Research University (PGNRU), M.Sc. of the Laboratory of Mechanobiology of Living Systems, Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Perm Federal Research Center of the Ural Branch of the Russian Academy of Sciences. - branch of the Perm Federal Research Center of the Ural RAS Department

A.V. Krivoruchko, Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Centre

Dr. B.Sc., Associate Professor, Head of the Laboratory of Mechanobiology of Living Systems, Institute of Ecology and Genetics of Microorganisms, Ural Branch of the Ural Federal Research Center of the Russian Academy of Sciences - branch of the Perm Federal Research Center of the Ural RAS Department

I.B. Ivshina, Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Centre

Doctor of Biology, Prof., Acad. of RAS. RAS, Head of the Laboratory of Alkanotrophic Microorganisms “Institute of Ecology and Genetics of Microorganisms, Ural RAS Department”, branch of Perm Federal Research Center, Ural RAS Department - branch of the Perm Federal Research Center of the Ural RAS Department

References

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Published
2024-11-02
How to Cite
Komarova L., Krivoruchko A., Ivshina I. EXPRESSION OF THE ALKB AND MMOAA GENES IN RHODOCOCCUS CELLS IN THE PRESENCE OF C3–C16 N-ALKANES // BIOAsia-Altai, 2024. Vol. 4, № 1. P. 108-112. URL: https://journal.asu.ru/bioasia/article/view/16140.
Issue
Vol 4 No 1 (2024): International Biotechnology Forum “BIOAsia-Altai”
Section
Biotechnology for Industry and Agriculture

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