EVALUATION OF THE ELECTROGENICITY OF RHODOCOCCUS ELECTRODIPHILUS ISOLATE USING AN ELECTROCHEMICAL BIOSENSOR

  • K.V. Revyakina S.P. Korolev Samara National Research University Email: anatol@ibpm.pushchino.ru
  • S.E. Tarasov G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Center for Biological Research of the Russian Academy of Sciences Email: anatol@ibpm.pushchino.ru
  • Y.V. Plekhanova G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Center for Biological Research of the Russian Academy of Sciences Email: anatol@ibpm.pushchino.ru
  • A.G. Bykov G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Center for Biological Research of the Russian Academy of Sciences Email: anatol@ibpm.pushchino.ru
  • G.V. Khokhlova G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Center for Biological Research of the Russian Academy of Sciences Email: anatol@ibpm.pushchino.ru
  • M.B. Wainshtein G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Center for Biological Research of the Russian Academy of Sciences Email: anatol@ibpm.pushchino.ru
  • A.N. Reshetilov G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center “Pushchino Center for Biological Research of the Russian Academy of Sciences Email: anatol@ibpm.pushchino.ru
Keywords: Rhodococcus electrodiphilus, electrogenicity, microbial biosensor, electrochemical properties, biocatalyst

Abstract

The paper discusses the electrochemical behavior of an isolate of Gram-positive microorganism Rhodococcus electrodiphilus upon its immobilization on a graphite screen-printed electrode. This microorganism was used as a biocatalyst for an amperometric biosensor, the possibility and conditions of electron transfer from these microorganisms to the electrode in the process of transformation of various substrates were studied. The influence of polymer gels and carbon nanomaterials on the activity of this microorganism and the possibility of its long-term operation as part of the biosensor was shown. The prospects of using R. electrodiphilus as a biocatalyst of biosensor for the detection of glucose and 2,6-dichlorophenolindophenol were evaluated.

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Published
2024-11-02
How to Cite
Revyakina K., Tarasov S., Plekhanova Y., Bykov A., Khokhlova G., Wainshtein M., Reshetilov A. EVALUATION OF THE ELECTROGENICITY OF RHODOCOCCUS ELECTRODIPHILUS ISOLATE USING AN ELECTROCHEMICAL BIOSENSOR // BIOAsia-Altai, 2024. Vol. 4, № 1. P. 181-184. URL: https://journal.asu.ru/bioasia/article/view/16168.
Section
Biotechnology for Industry and Agriculture