DEVELOPMENT OF A REAL-TIME PCR TEST SYSTEM FOR THE IDENTIFICATION OF SOUTHERN BEAN MOSAIC VIRUS
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Keywords

PCR
virus
soy
bean
diagnosis

How to Cite

Bondarenko N., Magomedova K., Shilkina N. DEVELOPMENT OF A REAL-TIME PCR TEST SYSTEM FOR THE IDENTIFICATION OF SOUTHERN BEAN MOSAIC VIRUS // BIOAsia-Altai, 2024. Vol. 4, № 1. P. 274-277. URL: https://journal.asu.ru/bioasia/article/view/16340.

Abstract

The article deals with Southern bean mosaic virus (SBMV), with special emphasis on phytosanitary risk for soybean exports from the Russian Federation. The main objective of the work is to develop a highly sensitive method of SBMV identification. In the course of the study, the literature data on biological and molecular-genetic characteristics of the pathogenic virus, as well as on the method of its detection and identification were summarized, previously developed primer systems were evaluated, and as a result, own primers for the target pathogen were created. The results obtained may contribute to the improvement of diagnostics and control of virus spread, which is important for agriculture and export potential of soybean in Russia.

PDF (Русский)

References

Verhoeven J.Th.J., Roenhorst J.W., Lesemann D.-E. et al. Southern bean Mosaic Virus the Causal Agent of a New Disease of Phaseolus vulgaris beans in Spain // European Journal of Plant Pathology. 2003. Vol. 109(9). P. 935–941. https://doi.org/10.1023/B:EJPP.0000003673.10046.2f

Lokesh G.L., Gopinath K., Satheshkumar P.S. et al. Complete nucleotide sequence of Sesbania mosaic virus: a new virus species of the genus Sobemovirus // Archives of Virology. 2001. Vol. 146(2). P. 209–223. https://doi.org/10.1007/s007050170170

Segundo E., Gil-Salas F.M., Janssen D. et al. First Report of Southern bean mosaic virus Infecting French Bean in Morocco // Plant Disease. 2004. Vol. 88(10). P. 1162. https://doi.org/10.1094/PDIS.2004.88.10.1162B

Tamm T., Truve E. Sobemoviruses // Journal of Virology. 2000. Vol. 74(14). P. 6231–6241. https://doi.org/10.1128/JVI.74.14.6231-6241.2000

Teakle D. Abiotic Transmission of Southern Bean Mosaic Virus in Soil // Australian Journal of Biological Sciences. 1986. Vol. 39(4). P. 353-349. https://doi.org/10.1071/BI9860353

Mulenga R.M., Chikoti P.C., Chiona M. et al. First Report of Southern bean mosaic virus infecting common bean in Zambia // Plant Disease. 2020. 104(6). https://doi.org/10.1094/PDIS-11-19-2390-PDN

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