OBTAINING THE REGENERANT PLANTS OF PINUS SYLVESTRIS L. PLUS CLONES

  • R.V. Ignatenko Forest Institute - a separate subdivision of the Federal State Budgetary Institution of Science Federal Research Center “Karelian Research Center of the Russian Academy of Sciences”. Email: galibina@krc.karelia.ru
  • M.A. Yershova Forest Institute - a separate subdivision of the Federal State Budgetary Institution of Science Federal Research Center “Karelian Research Center of the Russian Academy of Sciences”. Email: galibina@krc.karelia.ru
  • O.V. Chirva Forest Institute - a separate subdivision of the Federal State Budgetary Institution of Science Federal Research Center “Karelian Research Center of the Russian Academy of Sciences”. Email: galibina@krc.karelia.ru
  • N.A. Galibina Forest Institute - a separate subdivision of the Federal State Budgetary Institution of Science Federal Research Center “Karelian Research Center of the Russian Academy of Sciences”. Email: galibina@krc.karelia.ru
Keywords: somatic embryogenesis, in vitro cluture, phytohormones, embryos, common pine, forest seed plantation, Karelia

Abstract

Donor plants of Pinus sylvestris explants from Petrozavodsk forest seed plantation capable of somatic embryogenesis initiation were studied, and the formation of embryogenic cell lines on MSG nutrient media with different content and sources of phytohormones was evaluated. The initiation of somatic embryogenesis was observed in explants selected from 2 out of 9 studied clones of plus trees. It was found that embryogenic cell masses were formed with low frequency on all substrates studied. The initial stages of somatic embryo maturation were recorded after seven days of incubation of cell masses on hormone-free medium and then on substrate with ABA. At the end of the experiment, regenerant plants of Pinus sylvestris could not be obtained.

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Published
2024-11-02
How to Cite
Ignatenko R., Yershova M., Chirva O., Galibina N. OBTAINING THE REGENERANT PLANTS OF PINUS SYLVESTRIS L. PLUS CLONES // BIOAsia-Altai, 2024. Vol. 4, № 1. P. 303-307. URL: https://journal.asu.ru/bioasia/article/view/16350.
Issue
Vol 4 No 1 (2024): International Biotechnology Forum “BIOAsia-Altai”
Section
Molecular-Genetic and Biotechnological Methods of Plant Research

Copyright (c) 2024 R.V. Ignatenko, M.A. Ershova, O.V. Chirva, N.A. Galibina

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