IN-VITRO METHOD FOR DETERMINING THE POLLEN VIABILITY OF CLARKIA SPECIES AND CULTIVARS
Abstract
Clarkia species of the genus Clarkia Pursh are valuable floral and ornamental plants for Siberia and Russia, which require a biotechnological approach to accelerate breeding. To determine the viability of Clarkia pollen, on which the processes of fertilization and seed development depend, we used in vitro germination of pollen in a solution of a synthetic osmotic agent, polyethylene glycol with a molecular weight of 6000 (PEG 6000). Compared to a sucrose solution in a 30% PEG 6000 solution with the addition of microelements to the medium in the following concentrations: boric acid H3BO3 - 0.01%, calcium nitrate CaNO3 - 0.03%, magnesium sulfate MgSO4 - 0.02%, potassium nitrate KNO3 – 0.01%. the energy activity and viability of pollen grains increases by 1.7–2 times. It has been established that energy activity, pollen viability, and pollen tube growth rate in the studied species and varieties of the genus Clarkia Pursh from three different sections (Rhodanthos, Godetia, and Phaeostoma) are sensitive to both the cultivation environment and the duration of cultivation time.
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