Keywords
agriculture waste
deep eutectic solvents
flavonoids
C,O-glycosylflavones
How to Cite
Abstract
Green waste from greenhouse production of cucumber (Cucumis sativus L.) contains various metabolites, including flavonoids from the group of C,O-glycosylflavones, which have biological activity. The present work presents the results of the development of a method for extraction of flavonoids from C. sativus herb using deep eutectic solvents (DES), among which the most effective was a mixture of choline chloride - dipropylene glycol - water. The optimal conditions of ultrasonic influence, DES composition, raw material:extractant ratio and other parameters on the efficiency of flavonoids extraction from plant raw materials were determined. After solid-phase extraction on Amberlite XAD-2 macroporous resin, the sum of flavonoids containing various C,O-glycosylflavones with a total content of 876.07 mg/g was obtained. This study was the first to demonstrate the efficiency of DES for the extraction of flavonoids from green wastes of C. sativus.
References
2. Serna-Vázquez J., Ahmad M.Z., Boczkaj G., Castro-Muñoz R. Latest insights on novel deep eutectic solvents (DES) for sustainable extraction of phenolic compounds from natural sources // Molecules. – 2021. – Vol. 26. – No. 5037. DOI: https://doi.org/10.3390/molecules26165037.
3. Hao Y., Pei P., Huang J., Li G. Application of deep eutectic solvents on extraction of flavonoids // J. Sep. Sci. – 2024. – Vol. 47. – No. 2300925. DOI: https://doi.org/10.1002/jssc.202300925.
4. Olennikov D.N. Separation, characterization and mammal pancreatic lipase inhibitory potential of cucumber flower flavonoids // Separations. – 2023. – Vol. 10. – No 255. DOI: https://doi.org/10.3390/separations10040255.
5. Olennikov D.N., Kashchenko N.I. New flavonoids from Cucumis sativus // Chem. Nat. Comp. – 2023a. – Vol. 59. – P. 651–654. DOI: https://doi.org/10.1007/s10600-023-04079-4.
6. Olennikov D.N., Kashchenko N.I. Acylated flavonoids from Cucumis sativus inhibit activity of human pancreatic lipase // Appl. Biochem. Microbiol. – 2023b. – Vol. 59. – P. 530–538. DOI: https://doi.org/10.1134/S0003683823040099.
7. Olennikov D.N., Kashchenko N.I. Green waste from cucumber (Cucumis sativus L.) cultivation as a source of bioactive flavonoids with hypolipidemic potential // Agronomy. – 2023c. – Vol. 13. – No 2410. DOI: https://doi.org/10.3390/agronomy13092410.
8. Olennikov D.N., Kashchenko N.I. New acylated C,O-glycosylflavones from Cucumis sativus // Chem. Nat. Comp. – 2024. – Vol. 60. – P. 235–240. DOI: https://doi.org/10.1007/s10600-024-04295-6.
This work is licensed under a Creative Commons Attribution 4.0 International License.