STRUCTURAL AND GENETIC STABILITY, ESSENTIAL OIL COMPONENT COMPOSITION OF THE HYSSOPUS OFFICINALIS FORMS IN VITRO AND EX SITU

  • I.V. Bulavin Nikita Botanical Gardens – National Scientific Center of the RAS Email: cellbiolnbs@yandex.ru
  • N.N. Miroshnichenko Nikita Botanical Gardens – National Scientific Center of the RAS Email: cellbiolnbs@yandex.ru
  • N.M. Saplev Nikita Botanical Gardens – National Scientific Center of the RAS Email: cellbiolnbs@yandex.ru
  • S.A. Feskov Nikita Botanical Gardens – National Scientific Center of the RAS Email: cellbiolnbs@yandex.ru
  • D.K. Soldatov Nikita Botanical Gardens – National Scientific Center of the RAS Email: cellbiolnbs@yandex.ru
  • V.D. Konobeev Nikita Botanical Gardens – National Scientific Center of the RAS Email: cellbiolnbs@yandex.ru
  • D.I. Kalmykova Nikita Botanical Gardens – National Scientific Center of the RAS Email: cellbiolnbs@yandex.ru
  • N.N. Ivanova Nikita Botanical Gardens – National Scientific Center of the RAS Email: cellbiolnbs@yandex.ru
Keywords: common hyssop, in vitro culture, anatomy, ploidy level, PCR-analysis, gas chromatography

Abstract

The paper presents the results of the structural investigation, ploidy level, and genetic similarity analysis of Hyssopus officinalis in vitro compared with ex situ plants, as well as data on the component composition of the essential oil of regenerants ex vitro. Variations in the number of leaf surface structures, qualitative and quantitative anatomical changes in organs, and structural changes at 6-benzylaminopurine elevated concentrations were established. The absence of genomic and genetic changes and variations in the component composition of the essential oil was shown.

References

Интродукция и селекция ароматических и лекарственных растений (Методологические и методические аспекты) / В.П. Исиков, В.Д. Работягов, Л.А. Хлыпенко, И.Е. Логвиненко, Л.А. Логвиненко, С.П. Кутько, Н.Н. Бакова, Н.В. Марко. Ялта: НБС–ННЦ, 2009. 110 с.

Крицкая Т.А., Кашин A.С., Касаткин М.Ю. Микроразмножение и сомаклональная изменчивость Tulipa suaveolens (Liliaceae) in vitro // Онтогенез. 2019. T. 50. № 4. C. 270–277.

Справочник по ботанической микротехнике. Основы и методы / Барыкина Р.П., Веселова Т.Д., Девятов А.Г., Джалилова Х.Х., Ильина Г.М., Чубатова Н.В. М.: Изд-во МГУ, 2004. 312 с.

Супрун И.И., Маляровская В.И., Степанов И.В., Самарина Л.С. IRAP-анализ для оценки генетической стабильности эндемичных и исчезающих видов флоры Западного Кавказа в коллекции in vitro // Вавиловский журнал генетики и селекции. 2019. Т. 23. № 1. С. 8–14.

Bairu M.W., Aremu A.O., Van Staden J. Somaclonal variation in plants: causes and detection methods // Plant Growth Regulation. 2011. V. 63. P. 147–173.

Bulavin I.V., Ivanova N.N., Mitrofanova I.V. In vitro regeneration of Hyssopus officinalis L. and plant genetic similarity // Doklady Biological Sciences. 2021. V. 499. № 1. P. 109−112.

Bulavin I.V., Ivanova N.N., Miroshnichenko N.N., Saplev N.M., Feskov S.A. Anatomy, ploidy level, and essential oil composition of Hyssopus officinalis ʻNikitskiy Beliyʼ in vitro and ex situ // Proceedings on Applied Botany, Genetics and Breeding. 2023. V. 184. № 4. P. 21−30.

Eshboev F., Karakozova M., Abdurakhmanov J., Bobakulov K., Dolimov K., Abdurashidov A., Baymirzaev A., Makhnyov A., Terenteva E., Sasmakov S., Piyakina G., Egamberdieva D., Nazarov P.A., Azimova S. Antimicrobial and сytotoxic activities of the secondary metabolites of endophytic fungi isolated from the medicinal plant Hyssopus officinalis // Antibiotics. 2023. V. 12. № 7. 1201.

Galán- Ávila A., García-Fortea E., Prohens J., Herraiz F.J. Development of a direct in vitro plant regeneration protocol from Cannabis sativa L. seedling explants: developmental morphology of shoot regeneration and ploidy level of regenerated plants // Frontiers in Plant Science. 2020. V. 11. Art. 645.

Kotyuk L.A. Features of micromorphological structure of medicinal hyssop // Modern Phytomorphology. 2016. V. 10. P. 59–67.

Manokari M., Priyadharshini S., Shekhawat M.S. Micro-structural stability of micropropagated plants of Vitex negundo L. // Microscopy and Microanalysis. 2021. V. 27. № 3. P. 626–634.

Martins J.P.R., Rodrigues L.C.D.A., Conde L.T., Gontijo A.B.P.L., Falqueto A.R. Anatomical and physiological changes of in vitro-propagated Vriesea imperialis (Bromeliaceae) in the function of sucrose and ventilated containers // Plant Biosystems. 2020. V. 154. № 1. P. 87–99.

Mitrofanova I., Tsyupka V., Jain S.M. Morpho-anatomical characterization of in vitro regenerated plants // Advances in Plant Tissue Culture. Current Developments and Future Trends / Eds. A.C. Rai, A. Kumar, A. Modi, M. Singh. Cambridge, MA: Academic Press, 2022. P.175–204

Neelakandan A.K, Wang K. Recent progress in the understanding of tissue culture-induced genome level changes in plants and potential applications // Plant Cell Rep. 2012. V. 31. № 4. P. 597–620.

Plugatar Y.V., Bulavin I.V., Ivanova N.N., Miroshnichenko N.N., Saplev N.M., Shevchuk O.M., Feskov S.A., Naumenko T.S. Study of the component composition of essential oil, morphology, anatomy and ploidy level of Hyssopus officinalis f. cyaneus Alef. // Horticulturae. 2023. V. 9. № 4. 480.

Sliwinska E., Thiem B. Genome size stability in six medicinal plant species propagated in vitro // Biologia Plantarum. 2007. V. 51. № 3. P. 556–558.

Zagorcheva T., Rusanov K., Rusanova M., Aneva I., Stancheva I., Atanassov I. Genetic and flower volatile diversity in two natural populations of Hyssopus officinalis L. in Bulgaria // Biotechnology & Biotechnological Equipment. 2020. V. 34. № 1. P. 1265−1272.

Published
2024-11-02
How to Cite
Bulavin I., Miroshnichenko N., Saplev N., Feskov S., Soldatov D., Konobeev V., Kalmykova D., Ivanova N. STRUCTURAL AND GENETIC STABILITY, ESSENTIAL OIL COMPONENT COMPOSITION OF THE HYSSOPUS OFFICINALIS FORMS IN VITRO AND EX SITU // BIOAsia-Altai, 2024. Vol. 4, № 1. P. 26-30. URL: https://journal.asu.ru/bioasia/article/view/16073.
Issue
Vol 4 No 1 (2024): International Biotechnology Forum “BIOAsia-Altai”
Section
Biotechnology for Industry and Agriculture

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