A new species of the genus Chrysina Kirby, 1828 is decribed from Panama. Chrysina valentini sp.n. is related to Chrysina tricolor (Ohaus, 1922) and Chrysina optima (H. W. Bates, 1888) in morphology and mtDNA COX I molecular marker. Three related species differ in elytra colour, elytral striae, and number of tubercles on foreleg femur. The morphological divergence of the compared species is supported by distances in mtDNA COI molecular marker.
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