Keywords
Quedius
wingless
DNA-barcoding
species delimination
How to Cite
Abstract
To assess the accuracy of the species composition of the Q. umbrinus complex proposed earlier by us (Salnitska and Solodovnikov 2021) using an integrative approach, we performed additional molecular analyses based on updated mitochondrial COI data and newly obtained nuclear wg data. Only DNA grade material was used in this study, as the combination of morphological and molecular data is necessary for effective species delimitation within this species complex. All phylogenetic analyses based on the separate COI and wg genes and the concatenated dataset confirmed the subdivision into four species: Q. umbrinus, Q. pseudoumbrinus, Q. sigwalti and Q. volkeri. The only discordance in the topologies between mitochondrial and nuclear genes was observed in the monophyly of Q. umbrinus complex which may be explained by conserved nature of nuclear gene. Most phylogenetic and species delimitation analyses revealed a significant subclade within Q. pseudoumbrinus comprising specimens from the Irkutsk Province, namely from the type locality of the already synonymized Q. angaricus. Even though the genetic distance of this subclade from other Q. pseudoumbrinus specimens reaches 4.1 %, we did not resurrect it due to the absence of the any distinct morphological characters.
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