Keywords
C-vallue
cytotype
flow cytometry
genome size database
herbarium
South Siberia
How to Cite
Abstract
Despite the global accumulation of genome size data, Siberia remains one of the most underrepresented regions in plant flow cytometry databases, creating a substantial geographic bias in comparative genomic research. To address this gap, we developed "Genome Altai, Plants" (https://genome.asu.ru/), the first public database of plant genome sizes in Russia, focused on the flora of Southern Siberia and adjacent territories. Genome size was estimated by flow cytometry with propidium iodide staining using internal standardization; herbarium specimens, silicagel-dried tissue, and fresh material from the living collection of the South-Siberian Botanical Garden were analysed. Each record is linked to a digitized voucher specimen in the ALTB Digital Herbarium, and for every measurement the internal standard and its assigned 2C value are specified, allowing recalculation between the classical Doležel and rice-based calibration systems. The database currently contains over 3,100 records covering 106 genera from 41 families. In this study, genome sizes are reported for 107 species from herbarium material and 124 species from the living collection, with 2C values ranging from 0.473 pg (Turritis glabra) to 156.48 pg (Galanthus lagodechianus). Because desiccation may distort DNA estimates by 15–20%, values obtained from dried material are flagged as provisional. The database supports ploidy interpretation, cytotype discovery, and biogeographic research, and will be regularly updated with new records and expanded taxonomic coverage.
References
Bainard JD, Husband BC, Baldwin SJ, Fazekas AJ, Gregory TR, Newmaster SG, Kron P (2011) The effects of rapid desiccation on estimates of plant genome size. Chromosome Research 19(6): 825–842. https://doi.org/10.1007/s10577-011-9232-5
Bennett MD, Smith JB (1976) Nuclear DNA amounts in angiosperms. Philosophical Transactions of the Royal Society of London B 274(933): 227–274. https://doi.org/10.1098/rstb.1976.0044
Bennett MD, Leitch IJ (1995) Nuclear DNA amounts in angiosperms. Annals of Botany 76(2): 113–176. https://doi.org/10.1006/anbo.1995.1085
Bennett MD, Leitch IJ (2005) Nuclear DNA amounts in angiosperms: progress, problems and prospects. Annals of Botany 95(1): 45–90. https://doi.org/10.1093/aob/mci003
Bennett MD, Leitch IJ (2011) Nuclear DNA amounts in angiosperms: targets, trends and tomorrow. Annals of Botany 107(3): 467–590. https://doi.org/10.1093/aob/mcq258
Doležel J, Sgorbati S, Lucretti S (1992) Comparison of three DNA fluorochromes for flow cytometric estimation of nuclear DNA content in plants. Physiologia Plantarum 85: 625–631. https://doi.org/10.1111/j.1399-3054.1992.tb04764.x
Doležel J, Doleželová M, Novák F (1994) Flow cytometric estimation of nuclear DNA amount in diploid bananas (Musa acuminata and M. balbisiana). Biologia Plantarum 36: 351–357. https://doi.org/10.1007/BF02920930
Doležel J, Greilhuber J, Lucretti S, Meister A, Lysák M, Nardi L, Obermayer R (1998) Plant genome size estimation by flow cytometry: inter-laboratory comparison. Annals of Botany 82: 17–26. https://doi.org/10.1093/oxfordjournals.aob.a010312
Garcia S, Leitch IJ, Anadon-Rosell A, Canela MÁ, Gálvez F, Garnatje T, Gras A, Hidalgo O, Johnston E, Mas de Xaxars G, Pellicer J, Siljak-Yakovlev S, Vallès J, Vitales D, Bennett MD (2014) Recent updates and developments to plant genome size databases. Nucleic Acids Research 42(D1): D1159–D1166. https://doi.org/10.1093/nar/gkt1195
Garnatje T, Canela MA, Garcia S, Pellicer J, Sánchez-Jiménez I, Siljak-Yakovlev S, Vitales D, Vallès J Pellicer J, Sánchez-Jiménez I, Siljak-Yakovlev S, Vitales D, Vallès J Pellicer J, Sánchez-Jiménez I, Siljak-Yakovlev S, Vitales D, Vallès J (2011) GSAD: A Genome Size in the Asteraceae Database. Cytometry A 79(6): 401–404. https://doi.org/10.1002/cyto.a.21060
Genome Altai, Plants (2025). Available from https://genome.asu.ru/
Hornych O, Ekrt L, Riedel F, Koutecký P, Košnar J (2019) Asymmetric hybridization in central European populations of the Dryopteris carthusiana group. American Journal of Botany 106: 1477–1486. https://doi.org/10.1002/ajb2.1369
Leitch IJ, Johnston E, Pellicer J, Hidalgo O, Bennett MD (2019) Plant DNA C-values Database (release 7.1, April 2019). Available from: https://cvalues.science.kew.org/
Loureiro J, Suda J, Doležel J, Santos C (2007) FLOWER: A Plant DNA Flow Cytometry Database. In: Doležel J, Greilhuber J, Suda J (Eds) Flow Cytometry with Plant Cells. Wiley-VCH, Weinheim, 423–438. https://doi.org/10.1002/9783527610921.ch18
Siljak-Yakovlev S, Pustahija F, Šolić EM, Bogunić F, Muratović E, Bašić N, Catrice O, Brown SC (2010) Towards a genome size and chromosome number database of Balkan flora: C-values in 343 taxa with novel values for 242. Advanced Science Letters 3: 190–213. https://doi.org/10.1166/asl.2010.1115
Skaptsov M (2026) GSAnalyser: an open-source tool for objective DNA content estimation and quality control in plant flow cytometry (Version v1.1.0). Zenodo. https://doi.org/10.5281/zenodo.23163447
Skaptsov M, Degtyareva G, Smirnov S, Kutsev M, Shmakov A (2026) Plant flow cytometry: current problems, aspects of application and data analysis. Ecological Genetics 24(3). https://doi.org/10.17816/ecogen707965
Skaptsov MV, Kutsev MG, Smirnov SV, Vaganov AV, Uvarova OV, Shmakov AI (2024) Standards in plant flow cytometry: an overview, polymorphism and linearity issues. Turczaninowia 27(2): 86–104. https://doi.org/10.14258/turczaninowia.27.2.6
Temsch EM, Koutecký P, Urfus T, Šmarda P, Doležel J (2022) Reference standards for flow cytometric estimation of absolute nuclear DNA content in plants. Cytometry Part A 101(9): 710–724. https://doi.org/10.1002/cyto.a.24495
Veselý P, Bureš P, Šmarda P, Pavlíček T (2012) Genome size and DNA base composition of geophytes: the mirror of phenology and ecology? Annals of Botany 109(1): 65–75. https://doi.org/10.1093/aob/mcr267
Wang G, Yang Y (2016) The effects of fresh and rapid desiccated tissue on estimates of Ophiopogoneae genome size. Plant Diversity 38(4): 190–193. https://doi.org/10.1016/j.pld.2016.08.001
Watanabe K (2004) Index to chromosome numbers in the Asteraceae on the web. Compositae Newsletter 41: 64.
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