Variability of morphometric characteristics of Dactylis glomerata L. (Pooideae Benth.) leaf phytoliths at two stages of the growing season


seasonal variability
leaf epidermis
Dactylis glomerata

How to Cite

Solomonova, M. Y., Blinnikov, M. S., Lyashchenko, A. D., & Zhembrovskaya, T. A. (2024). Variability of morphometric characteristics of Dactylis glomerata L. (Pooideae Benth.) leaf phytoliths at two stages of the growing season. Acta Biologica Sibirica, 10, 633–648.


The study is devoted to the analysis of the morphometric parameters of Crenate phytoliths of the Dactylis glomerata L. leaf epidermis. The use of morphometry for the nomenclature and identification of phytoliths determines the relevance of studying factors that impact on the size of silica bodies. One of the possible reasons for morphometric differences may be the phenophase in which the herbarium material was collected. We collected herbarium material of D. glomerata from its two habitats at the beginning and the end of the growing season. Morphometric data of phytoliths extracted from leaves were processed using descriptive statistics and analysis of variance. The Crenate study of D. glomerata phytoliths at different stages of the growing season shows that some parameters tend to increase, while others remain stable and may have a taxonomic potential. Finally, the increase in the size characteristics of phytoliths at the end of the growing season has been revealed. Such parameters as area, width and equivalent diameter are stable. By the end of the growing season, the phytoliths have a more elongated and irregular shape. The most stable shape parameters are roundness, compactness and aspect ratio.


Attolini D, Pattelli L, Nocentini S, Wiersma DS, Tani C, Papini A, Mariotti Lippi M (2023) Developmental analysis and optical modelling of short cell phytoliths in Festuca exaltata (Poaceae). Flora 301: 152239.

Ball TB, Brotherson JD (1992) The effect of varying environmental conditions on phytolith morphometries in two species of grass (Bouteloua curtipendula and Panicum virginatum). Scanning Microscopy 6(4): 1163–1181.

Ball TB, Gardner JS, Anderson N (1999) Identifying inflorescence phytoliths from selected species of wheat (Triticum monococcum, T. dicoccon, T. dicoccoides, and T. aestivum) and barley (Hordeum vulgare and H. spontaneum). American Journal of Botany 86: 1615–1623.

Ball TB, Gardner JS, Bortherson JD (1996) Identifying phytoliths produced by the inflorescence bracts of three species of wheat (Triticum monococcum L., T. dicoccum Schrank., and T. aestivum L.) using computer-assisted image and statistical analyses. Journal of Archaeological Science 23: 619–632.

Ball TB, Davis A, Evett RR, Ladwig JL, Tromp M, Out WA, Portillo M (2016) Morphometric analysis of phytoliths: recommendations towards standardization from the International Committee for Phytolith Morphometrics. Journal of Archaeological Science 68: 106–111.

Ball TB, Vrydaghs L, Mercer T, Pearce M, Snyder S, Lisztes-Szabó Z, Pető A (2017) A morphometric study of variance in articulated dendritic phytolith wave lobes within select- ed species of Triticeae and Aveneae. Vegetation History and Archaeobotany 26: 85–97.

Blackman E (1969) Observations on the development of the silica cells of the leaf sheath of wheat (Triticum aestivum). Canadian Journal of Botany 47: 827–838.

Blinnikov M S (2005) Phytoliths in plants and soils of the interior Pacific Northwest, USA. Review of Palaeobotany and Palynology 135: 71–98.

Bobrov AA, Bobrova EK, Alexeev JuE (2001) Biogenic silica in biosystematics – potential uses. In: Meunier JD, Colin F (Eds) Phytoliths: Applications in Earth Sciences and Human History. Lisse, Swets end Zeitlinger, 279–288.

Chen QLiY, Ma Y, Zhou Z, Yang X (2023) Rice use history in Southeast China: Phytolith evidence from the Nanshan site in Fujian Province. Science China Earth Sciences 66: 1108–1119.

Dunn RE, Le T-YT, Strömberg CAE (2015) Light environment and epidermal cell morphology in grasses. International Journal of Plant Sciences 176(9): 832–847.

Esau K (1965) Plant Anatomy. Second edition. Wiley, New York, 735 pp.

Fernandez Honaine M, Benvenuto ML, Borrelli NL, Osterrieth M (2016) Early silicification of leaves and roots of seedlings of a panicoid grass grown under different conditions: anatomical relations and structural role. Plant Biology 18: 1025–1030.

Gavrilov DA, Golyeva AA (2014) Microbiomorphic research of soils with the second humus horizon of the West Siberian southern taiga subzone (Russia). Tomsk State University Journal of Biology 2 (26): 5–22. [In Russian with English abstract]

Golyeva AA (2001) Phytoliths and their informational role in the study of natural and archaeological objects. Poltex, Moscow, Elista; Syktyvkar, 140 pp. [In Russian]

Hodson MJ, Sangster AG, Parry DW (1985) An ultrastructural study on the developmental phases and silicification of the glume of Phalaris canariensis L. Annals of Botany 55: 649–655.

Hošková K, Neustupa J, Pokorný, P, Pokornáet A (2022) Phylogenetic, ecological and intraindividual variability patterns in grass phytolith shape. Annals of Botany 129(3): 303–314.

Hošková K, Pokorná A, Neustupa J, Pokorný P (2021) Inter- and intraspecific variation in grass phytolith shape and size: a geometric morphometrics perspective. Annals of Bot- any 127: 191–201.

Issaharou-Matchi I, Barboni D, Meunier J-D, Saadou M, Dussouillez P, Contoux C, Zirihi- Guede N (2016) Intraspecific biogenic silica variations in the grass species Pennisetum pedicellatum along an evapotranspiration gradient in South Niger. Flora 220: 84–93.

Kiseleva NK (1989) Phytolithic analysis of zoogenic deposits and buried soils. In: Dinesman L G, Kiseleva N K, Knyazev AV (Eds) History of steppe ecosystems of the Mongolian People's Republic. Nauka, Moscow, 15–36. [In Russian]

Lada NYu, Gavrilov DA (2016) Analysis of phytolith composition of the main plant steppe ecosystems of Western Siberia. Tomsk State University Journal of Biology 2(34): 53–68. [In Russian, English summary].

Lisztes-Szabo Z, Kovacs S, Pető A (2014) Phytolith analysis of Poa pratensis (Poaceae) leaves. Turkish Journal of Botany 38(5): 851–863.

Liu L, Jie D, Liu H, Gao G, Gao Z, Li D, Li N, Qiao Z, Gu J (2016a) Response of phytoliths in Phragmites australis to environmentalfactors in northeast China. Ecological Engineering 92(2016) 119–131.

Liu L, Jie D, Liu H, Gao Z, Gao G, Li N, Guo J, Qiao Z (2016b) An orthogonal experimental study of phytolith size of Phragmites communis in northeast China. Boreas 45(1): 122–132.

Lu H, Liu K-b (2003) Phytoliths of common grasses in the coastal environments of southeastern USA. Estuarine, Coastal and Shelf Science 58(3): 587–600.

Neumann K, Strömberg C, Ball T, Albert RM, Vrydaghs L, Cummings LS (2019) Interna- tional Code for Phytolith Nomenclature (ICPN) 2.0. Annals of Botany 124: 189–199.

Out WA, Madella M (2016) Morphometric distinction between bilobate phytoliths from Panicum miliaceum and Setaria italica leaves. Archaeological and Anthropological Sciences 8(3): 505–521.

Piperno DR (2006) Phytoliths: A Comprehensive Guide for Archaeologists and Paleoecologists. Altamira Press, Lanham, MD, 226 pp.

Portillo M, Ball TB, Manwaring J (2006) Morphometric analysis of inflorescence phytoliths produced by Avena sativa L. and Avena strigosa schreb. Economic Botany 60(2): 121–129.[121:MAOIPP]2.0.CO;2

Rudall PJ, Prychid C, Gregory T (2014) Epidermal patterning and silica phytoliths in Grasses: An evolutionary history. The Botanical Review 80 (1): 59–71.

Solomonova MY, Blinnikov MS, Silantieva MM, Speranskaja NJ (2019a) Influence of moisture and temperature regimes on the phytolith assemblage composition of mountain ecosystems of the mid latitudes: a case study from the Altay Mountains. Frontiers in Ecology and Evolution 7: 2.

Solomonova МYu, Blinnikov МS, Speranskaja NY, Elesova NV, Silantyeva MM (2019b) Phytolith assemblages in modern top soils under plant communities of Northern and Western Altay, Russia. Ukrainian Journal of Ecology 9(3): 429–435.

Solomonova MY, Silant’eva MM, Kiryushin KY (2016) Transformation of plant cover on the territory of the Tytkesken’-2 settlement (Altai mountains) from the fourth century BC to the present day according to paleobotanical data. Biology Bulletin 43(10): 1434– 1439.

Solomonova MY, Speranskaya NY, Blinnikov MS, Kharitonova EY, Pechatnova YV, Silan- tieva MM (2018) Cyperaceae Juss. and Juncaceae A. Rich ex Kunt. phytoliths of Western Siberia. Ukrainian Journal of Ecology 8(4): 332–334.

Solomonova MY, Speranskaya NY, Blinnikov MS, Zhembrovskaya TA, Silantyeva MM (2022) Separation of wavy and polylobate forms of phytoliths of the “crenate” morphotype in Pooideae Benth. species of the south of Western Siberia on the basis of phylogenetic data. Turczaninowia 25(4): 122–135.

Solomonova MY, Zhembrovskaya TA, Lyashchenko AD, Kotov SD, Speranskaya NY (2023) Environmental impact on phytolith morphometric parameters by example crenate morphotype of Dactylis glomerata L. leaves (South of Western Siberia, Russia). Acta Biologica Sibirica 9: 953–973.

Speranskaya NYu, Solomonova MYu, Geinrich YuV, Silantyeva MM (2018) Higher plant’s phytoliths on south of Western Siberia. Problems of botany of South Siberia and Mongolia 17(1): 313–317. [In Russian, English summary]

Twiss PC (2001) A curmudgeon’s view of grass phytolithology. In: Meunier J-D, Colin F (Eds) Phytoliths: Applications in Earth Sciences and Human History. Balkema, Leiden, 7–25.

Twiss PC, Suess E, Smith RM (1969) Morphological classification of grass phytoliths. Soil Science Society of America Proceedings 33: 109–115.

Wang C, Lu H, Zhan J, Mao L, Ge Y (2019) Bulliform phytolith size of rice and its correlation with hydrothermal environment: a preliminary morphological study on species in Southern China. Frontiers in Plant Science 10: 1037.

Yost CL, Michas MC, Adams KR, Swarts K, Puseman K, Ball T (2021) An in situ and morphometric study of maize (Zea mays L.) cob rondel phytoliths from Southwestern North American landraces. Journal of Archaeological Science: Reports 35.

Zhijun Z, Pearsall DM, Benfer R, Piperno DR (1998) Distinguishing rice (Oryza sativa Poaceae) from wild Oryza species through phytolith analysis, II: Finalized method. Economic Botany 52(2): 134–145.

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