QUANTIFICATION OF TOTAL FLAVONOID CONTENT IN THE AERIAL PART AND IN THE TINCTURE OF IRIS LACTEA (IRIDACEAE)
Abstract
Iris lactea Pall.of Iridaceae Juss. family contains a complex of biologically active substances with antiviral, immunomodulatory, adaptogenic, antihypoxic, cardiostimulatory and antitumor properties, Thus, the herbal substance and dosage forms on its basis are promising in medicine.
The main groups of active substances in the aerial part of Iris Lactea are flavonoids and xanthones. There is a known method of chromatospectrophotometric quantification of total xanthone content calculated as alpisarin. Meanwhile, the development of a convenient method of quantification of total flavonoid content in the aerial part of Iris lactea and drugs on its basis is currently important.
The aims of this work are to develop a method of quantification of total flavonoid content calculated as apigenin in the aerial part of Iris lactea and in its tincture, using the differential spectrophotometry method according to the complexation reaction with aluminium chloride solution;to prove reliability and accuracy of this method for the analysis of Iris Lactea tincture in terms of the following factors: specificity, linearity, validity, precision, and repeatability.
As a result of verification data analysis, the developed method of quantification of total flavonoid content calculated as apigenin can be admitted for standardization of Iris Lactea herbal substance and tincture.
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Iris lactea Pall. – The Plant List. [Electronic resource]. URL: http://www.theplantlist.org/tpl1.1/record/kew-322026 (ac-cessed date – 05.06.2017).
Kasatik molochno-belyi – Iris lactea. Plantarium. [Iris milky-white – Iris lactea. Plantarium]. [Electronic resource]. URL: http://www.plantarium.ru/page/view/item/20763.html (accessed date – 05.06.2017). (in Russ.).
Alekseeva N.B. Turczaninowia, 2008, vol. 11, no. 2, pp. 5–68.
Priakhina N.I. Fitokhimicheskoe izuchenie kasatika molochno-belogo: avtoref. dis. ... kand. farm. nauk. [Phytochemical study of iris milky white: dissertation of the candidate of pharmaceutical sciences]. Leningrad, 1984, 25 p. (in Russ.).
Luzhanin V.G., Whaley А.К. Reviews of clinical pharmacology and drug therapy, 2016, vol. 14S, p. 42.
Whaley A.K., Ebrahim W., El-Neketi M., Ancheeva E.U., Özkaya F.C., Pryakhina N.I., Sipkina N.U., Luzhanin V.G., Liu Z., Proksch P. Tetrahedron. Lett., 2017, vol. 58, no. 22. pp. 2171–2173.
Meng Y., Qin M., Qi B., Xie G. Phytochem. Lett., 2017, vol. 22, pp. 33–38.
Shen W.J., Qin M.J., Shu P., Zhang C. F. Chin. Chem. Lett., 2008, vol. 19, no. 7, pp. 821–824.
Lv H., Wang H., He Y., Ding C., Wang X., Suo Y. J. of Chrom. B, 2015, vol. 988, pp. 127–134.
Lv H., Zhou W., Wang X., Wang Z., Suo Y., Wang H. J. of Chrom. Sci., 2016, vol. 54, no 5, pp. 744–751.
Lv H., Yuan Z., Wang X., Wang Z., Suo Y., Wang H. Phytochem. Anal., 2015, vol. 26, no. 6, pp. 444–453.
Lanina N.E., Minina S.A., Priakhina N.I. Rastitel'nye resursy, 2003, no. 3, pp. 99–105. (in Russ.).
Tikhomirova L.I., Bazarnova N.G., Mikushina I.V., Dolganova Z.V. Khimiya Rastitel'nogo Syr'ya, 2015, no. 3, pp. 25–34. (in Russ.).
Barinov E.A. Izuchenie immunomoduliruyushchih i adaptogennyh ehffektov SEHKMB pri gipoksii i gipertermii: avtoref. dis. ... kand. farm. nauk [Study of immunomodulating and adaptogenic effects of SSCMB in hypoxia and hy-perthermia: dissertation of the candidate of pharmaceutical sciences]. St. Petersburg, 1999, 20 p. (in Russ.).
Patent 2123349 (RU). 1998. (in Russ.).
Mel'nikova T.I. Farmakologicheskoe izuchenie summarnogo ekstrakta kasatika molochno-belogo : avtoref. dis. ... kand. biol. nauk. [Pharmacological study of the total extract of iris milky white: dissertation of the candidate of biologi-cal sciences]. St. Petersburg, 1994, 20 p. (in Russ.).
Gamal R. Tekhnologiya i analiz tabletirovannogo suhogo ehkstrakta kasatika molochno-belogo: avtoref. dis. ... kand. farm. nauk. [Technology and analysis of the tablet dry extract of milky white tangy (Iris lactea Pall.): dissertation of the candidate of pharmaceutical sciences]. St. Petersburg, 1996, 21 p. (in Russ.).
Minina S.A., Skhela G.R.I. Abu. Pharm. Chem. J., 1999, vol. 3, no 4, pp. 211–213.
Minina S.A., Astakhova T.V., Priakhina N.I., Abu Skhela G. Khimiko-farmatsevticheskii zhurnal, 2001, vol. 35, pp. 24–26. (in Russ.).
Minina S.A., Pryakhina N.I., Chemesova I.I., Chizhikov D.V. Khimiko-farmatsevticheskii zhurnal, 2008, vol. 42, no. 1, pp. 37–39. (in Russ.).
Minina S.A., Abu Skhela G.R., Astakhova T.V., Priakhina N.I., Zenkevich I.G., Kosman V.M. Khimiko-farmatsevticheskii zhurnal, 1999, no. 4 (33), pp. 40–42. (in Russ.).
Astakhova T.V., Priakhina N.I., Lanina N.E., Zazhigalkina M.V., Chemesova I.I. Aktual'nye problem farmatsevtich-eskoi nauki i obrazovaniia. [Actual problems of pharmaceutical science and education]. Perm, 2000, pp. 170–171. (in Russ.).
Lanina N.Y. Razrabotka tekhnologii nastojki iz travy kasatika molochno-belogo i eyo standartizaciya: avtoref. dis. ... kand. farm. nauk. [Development of technology of tincture from the herb of milky-white tang and its standardization: dissertation of the candidate of pharmaceutical sciences]. St. Petersburg, 2003, 23 p. (in Russ.).
Aroyan M.V., Kaukhova I.E., Novikova E.K., Goncharova S.B. Reviews of clinical pharmacology and drug therapy, 2017, vol. 15S, pp. 50.
Gosudarstvennaia farmakopeia Rossiiskoi Federatsii, XIII izdanie. T. 1. Statisticheskaia obrabotka rezul'ta-tov khimicheskogo eksperimenta. [State Pharmacopoeia of the XIII edition. Vol. 1. Statistical processing of the results of a chemical experiment]. Moscow, 2015, pp. 235–265. (in Russ.)
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