CHEMICAL PROFILING OF PAPAVER KUVAJEVII: DETERMINATION OF THE MAIN CYANOGENIC GLYCOSIDE – TAXIFILLIN
Abstract
The complex of plant metabolites as a set of certain substances can be characterized by chemical and physicochemical methods of analysis. A chemical characteristic involves the identification of a set of individual substances - chemical markers unique to a selected plant object; and the entire visible set of components as a characteristic profile – a "chemical fingerprint".
The object of the study is the poppy of Kuvaev (Papaver kuvajevii). A new kind of poppy; described in 2003. Endemic. Perennial. It grows in the steppe belt on the slopes of the mountains; rocks; screes; pasture steppes. Blossoms in May – August. Seed multiplication June – September.
In this paper; a scheme of extraction procedures for the spectroscopic profiling of the Kuvaev poppy has been developed. The crushed air-dry aerial part of the plant (without capsules) was extracted in the Soxhlet apparatus sequentially with solvents (in order of increasing their polarity). IR; NMR 1H and 13C profiles were recorded for each extract. The content of taxifillin in the sample is determined by quantitative NMR analysis (q-NMR).
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