LIPID COMPOSITION AND MEMBRANOPROTECTIVE ACTION OF EXTRACT FROM MARINE GREEN ALGAE ULVA LACTUCA (L.)

  • Svetlana Yevgen'yevna Fomenko V.I. Il’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences http://orcid.org/0000-0002-0261-0190 Email: fomenko29@mail.ru
  • Natal'ya Fedorovna Kushnerova V.I. Il’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences Email: natasha50@mail.ru
  • Vladimir Gennad'yevich Sprygin V.I. Il’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences Email: vsprygin@poi.dvo.ru
  • Elena Sergeyevna Drugova V.I. Il’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences Email: dryg-2005.84@mail.ru
  • Larisa Nikolayevna Lesnikova V.I. Il’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences Email: lesnicova@poi.dvo.ru
  • Valeriy Yur'yevich Merzluakov V.I. Il’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences Email: vum77@mail.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.2019035116
Keywords: lipid fraction from Ulva lactuca, essential, carbon tetrachloride, erythrocytes, lipids, rats

Abstract

The object of the present study was a water-alcohol extract obtained from the dried thallus of the marine green alga Ulva lactuca (L.) (syn.: Ulva fenestrate P. et R.) – ulva lettuce. Glycolipids (40.6%) and neutral lipids (34%) prevailed in the lipid fraction of the extract; phospholipids contained 13.4% of the total lipids. The content of polyunsaturated fatty acids (PUFA) was 50% of the total amount of fatty acids, among which PUFA of the n-3 family predominated (37.43%). On the model of toxic hepatitis induced by the introduction of carbon tetrachloride (CCl4) (50% solution in olive oil, subcutaneously 2 ml/kg for 4 days), we study the effect of the lipid fraction of U. lactuca extract and the commercial reference preparation Essentiale® on the physiological and biochemical characteristics of erythrocytes and lipid composition of erythrocyte membranes in rats. The introduction of the lipid fraction from the ulva (dose 80 mg of total lipids per kg of body weight) to the animals intragastrically for 7 days after withdrawal of CCl4 exerted a protective effect, which was manifested in the restoration of the erythrocyte size characteristics (average volume and diameter), their osmotic resistance to hemolysis, levels of malondialdehyde and reduced glutathione, as well as maintaining of the ratio of phospholipid fractions. Under the damaging effects of CCl4, the lipid fraction from the green alga U. lactuca was not inferior to the effectiveness of reference preparation Essentiale® in restoring of the physiological characteristics of erythrocytes and the phospholipid composition of its membranes.

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Author Biographies

Svetlana Yevgen'yevna Fomenko, V.I. Il’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences

кандидат биологических наук, ведущий научный сотрудник лаборатории биохимии

Natal'ya Fedorovna Kushnerova, V.I. Il’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences

доктор биологических наук, профессор, заведующая лабораторией биохимии

Vladimir Gennad'yevich Sprygin, V.I. Il’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences

кандидат биологических наук, ведущий научный сотрудник лаборатории биохимии

Elena Sergeyevna Drugova, V.I. Il’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences

кандидат биологических наук, младший научный сотрудник лаборатории биохимии

Larisa Nikolayevna Lesnikova, V.I. Il’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences

кандидат биологических наук, научный сотрудник лаборатории биохимии

Valeriy Yur'yevich Merzluakov, V.I. Il’ichev Pacific Oceanological Institute, Far Eastern Branch, Russian Academy of Sciences

научный сотрудник лаборатории биохимии

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Published
2019-02-12
How to Cite
1. Fomenko S. Y., Kushnerova N. F., Sprygin V. G., Drugova E. S., Lesnikova L. N., Merzluakov V. Y. LIPID COMPOSITION AND MEMBRANOPROTECTIVE ACTION OF EXTRACT FROM MARINE GREEN ALGAE ULVA LACTUCA (L.) // chemistry of plant raw material, 2019. № 3. P. 41-51. URL: http://journal.asu.ru/cw/article/view/5116.
Issue
No 3 (2019)
Section
Low-molecular weight compounds

Copyright (c) 2019 Khimiia rastitel'nogo syr'ia (Chemistry of plant raw material)

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