LIPID COMPOSITION AND MEMBRANOPROTECTIVE ACTION OF EXTRACT FROM MARINE GREEN ALGAE ULVA LACTUCA (L.)
Abstract
The object of the present study was a water-alcohol extract obtained from the dried thallus of the marine green alga Ulva lactuca (L.) (syn.: Ulva fenestrate P. et R.) – ulva lettuce. Glycolipids (40.6%) and neutral lipids (34%) prevailed in the lipid fraction of the extract; phospholipids contained 13.4% of the total lipids. The content of polyunsaturated fatty acids (PUFA) was 50% of the total amount of fatty acids, among which PUFA of the n-3 family predominated (37.43%). On the model of toxic hepatitis induced by the introduction of carbon tetrachloride (CCl4) (50% solution in olive oil, subcutaneously 2 ml/kg for 4 days), we study the effect of the lipid fraction of U. lactuca extract and the commercial reference preparation Essentiale® on the physiological and biochemical characteristics of erythrocytes and lipid composition of erythrocyte membranes in rats. The introduction of the lipid fraction from the ulva (dose 80 mg of total lipids per kg of body weight) to the animals intragastrically for 7 days after withdrawal of CCl4 exerted a protective effect, which was manifested in the restoration of the erythrocyte size characteristics (average volume and diameter), their osmotic resistance to hemolysis, levels of malondialdehyde and reduced glutathione, as well as maintaining of the ratio of phospholipid fractions. Under the damaging effects of CCl4, the lipid fraction from the green alga U. lactuca was not inferior to the effectiveness of reference preparation Essentiale® in restoring of the physiological characteristics of erythrocytes and the phospholipid composition of its membranes.
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Fukoidany – sul'fatirovannyye polisakharidy burykh vodorosley. Struktura, fermentativnaya transformatsiya i biolog-icheskiye svoystva [Fucoidans – sulfated polysaccharides of brown algae. Structure, enzymatic transformation and bio-logical properties], ed. N.N. Besednovoy, T.N. Zvyagintsevoy. Vladivostok, 2014, 380 p. (in Russ.).
Kumar C.S., Ganesan P., Suresh P.V., Bhaskar N. J. Food Sci. Technol., 2008, vol. 45, pp. 1–13.
Mayer A.M.S., Haman M.T. Comp. Biochem. Physiol., 2005, vol. 140, pp. 265–286, DOI: 10.1016/j.cca.2005.04.004.
Vinogradova K.L. Opredelitel' vodorosley dal'nevostochnykh morey SSSR. Zelenyye vodorosli. [Key to algae of the Far Eastern seas of the USSR. Green algae]. Leningrad, 1979, 147 p. (in Russ.).
Sprygin V.G., Kushnerova N.F., Fomenko S.Ye., Sizova L.A. Izvestiya Samarskogo nauchnogo tsentra RAN, 2012, vol. 14, no. 1(7), pp. 2299–2302. (in Russ.).
Fomenko S.E., Kushnerova N.F., Sprygin V.G., Momot T.V. Russian Journal of Marine Biology, 2016, vol. 42, no. 6, pp. 509–514, DOI: 10.1134/S1063074016060031.
Sprygin V.G., Fomenko S.Ye., Kushnerova N.F. Fundamental'nyye issledovaniya, 2014, no. 8 (1), pp. 110–114. (in Russ.).
Catala A. Chem. Phys. Lipids., 2009, vol. 157, pp. 1–11, DOI: 10.1016/j.chemphyslip.2008.09.004.
Kholari F.S., Dehpour A.R., Nourbakhsh M. et al. Acta Medica Iranica, 2016, vol. 54, no. 10, pp. 631–639.
Bligh E.G., Dyer W.J. Can. J. Biochem. Physiol., 1959, no. 37, pp. 911–917, DOI: 10.1139/o59-099.
Vaskovsky V.E., Khotimchenko S.V. J. Chromatography, 1982, vol. 5, pp. 635–636, DOI: 10.1002/jhrc.1240051113.
Vascovsky V.E., Kostetsky E.Y., Vasendin I.M. J. Chromatography, 1975, vol. 114, pp. 129–141, DOI: 10.1016/S0021-9673(00)85249-8.
Van Gent C.M., Roseleur O.J., Van der Bijl P. J. Chromatography, 1973, vol. 85, pp. 174–176, DOI: 10.1016/S0021-9673(01)91884-9.
Rouser G., Kritchevsky G., Yamamoto A. Lipid chromatographic analysis, New York: Dekker, 1967, vol. 1, pp. 99–162.
Keyts M. Tekhnika lipidologii. [Technique of lipidology]. Moscow, 1975, 322 p. (in Russ.).
Svetaсhev V.I., Vaskovsky V.Е. J. Chromatography, 1972, vol. 6, pp. 376–378, DOI: 10.1016/S0021-9673(01)91245-2.
Amenta J.S. J. Lipid Res., 1964, vol. 5, pp. 270–272.
Carreau J.P., Dubacо J.P. J. Chromatogr., 1978, vol. 151, pp. 384–390, DOI: 10.1016/S0021-9673(00)88356-9.
Christie W.W. J. Chromatogr., 1988, vol. 447, pp. 305–314, DOI: 10.1016/0021-9673(88)90040-4.
Vengerovskiy A.I., Markova I.V, Saratikov A.S. Vedomosti farm. komiteta, 1992, no. 2, pp. 9–12. (in Russ.).
Saratikov A.S., Rat'kin A.V., Frolov V.N., Chuchalin V.S. Voprosy biologicheskoy, meditsinskoy i farmatsevticheskoy khimii, 2004, no. 2, pp. 43–47. (in Russ.).
Endryu B.L. Eksperimental'naya fiziologiya. [Experimental physiology]. Moscow, 1972, 324 p. (in Russ.).
Folch J., Less M., Sloane-Stanley G.H. Biol. Chem., 1957, vol. 226, pp. 497–509.
Novgorodtseva T.P., Endakova E.A., Yan'kova V.I. Rukovodstvo po metodam issledovaniya parametrov sistemy "Perekisnoye okisleniye lipidov – antioksidantnaya zashchita" v biologicheskikh zhidkostyakh. [Guide to methods for studying the parameters of the system "Lipid peroxidation – antioxidant protection" in biological fluids]. Vladivostok, 2003, 80 p. (in Russ.).
Goncharova S.N., Kostetsky E.Y., Sanina N.M. Russ. J. Plant Physiol., 2004, vol. 51, no. 2, pp. 169–175, DOI: 10.1023/B:RUPP.0000019209.10747.9d.
Dembitsky V.M., Rozetsvet O.A. Phytochemistry, 1989, vol. 28, pp. 3341–3343, DOI: 10.1016/0031-9422(89)80343-7.
Khotimchenko S.V. Lipids of marine Macrophytic Algae and Grasses: Structure, Distribution, Analysis, Vladivostok: Dalnauka, 2003.
Sanina N.M., Goncharova S.N., Kostetsky E.Y. Phytochemistry, 2008, vol. 69, pp. 1517–1527, DOI: 10.1016/j.phytochem.2008.01.014.
Fadeel B., Xue D. Critical Reviews In Biochemistry & Molecular Biology, 2009, vol. 44, pp. 264–277, DOI: 10.1080/10409230903193307.
Boll M., Lutz W.D., Becker E., Stampfl A. Z. Naturforsch., 2001, vol. 56, no. 7–8, pp. 649–659, DOI: 10.1515/znc-2001-7-826.
Manibusan M.K., Odin M., Eastmond D.A. J. Environ. Sci. Health. C Environ Carcinog Ecotoxicol Rev., 2007, vol. 25, no. 3, pp. 185–209, DOI: 10.1080/10590500701569398.
Akyuz F., Aydin O., Demir T.A. et al. Biol. Trace Elem. Res., 2009, vol. 132, no. 1–3, pp. 207–214, DOI: 10.1007/s12011-009-8395-9.
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