ACCUMULATION OF CHEMICAL ELEMENTS IN TISSUES OF MEDICINAL PLANTS IN VITRO CULTURE
UDC 581.192.1:582.734
Abstract
Development of theoretical bases of optimization of the production process of biologically active substances in the cultivation of medicinal plants by modern methods of biotechnology is one of the main tasks of fundamental research in this area.
The aim of this study was to study the content and features of the accumulation of elements in regenerants of medicinal plants grown on the nutrient medium Murashige-Skoog with different content of phytohormones.
Regenerant plants in vitro culture actively accumulate elements (K, P, Ca, Mg, Na, Zn, Mn, Fe, B, Al, Cu, Pb, Sr, V, Cr, Ag, Mo, Ti, Ni, Co , Se, Cd). The content of the trace elements Mn, Zn and Cu that we studied was at the level of normal values for the vegetation of the continents, Fe in P. alba regenerants – much higher. The content of heavy and toxic metals Pb, Cd, As, Cr, Ni did not exceed the normal level in plants and the acceptable level for dietary supplements, plant-based tea, and medicinal plant materials.
The high dependence of the accumulation of elements in plants-regenerants Iris spuria Art And Soul variety on the content of hormones in the nutrient medium was noted. Representatives of all three groups (macronutrients, trace elements and ultramicroelements) had a similar curve diagram. The maximum peak was located at a concentration of 2.5 µm BAP with auxins, then the coefficient decreased by 5 µm BAP, and the next lower peak was observed at 5.0 µm BAP+A. the Lowest value of KN was at a concentration of 7.5 µm BAP. The introduction of auxins at this BAP concentration stimulated another peak, and then the accumulation coefficient tended to increase at a concentration of 10.0 µm BAP. The revealed dependence makes possible the regulation and directed accumulation of elements necessary for the researcher in plant tissues.
Downloads
Metrics
References
Butenko R.G. Biologiya kletok vysshikh rasteniy in vitro i biotekhnologiya na ikh osnove. [Biology of cells of higher plants in vitro and biotechnology based on them]. Moscow, 1999, 152 p. (in Russ.).
Zaprometov M. Advances in Cell Culture, 1989, vol. 7, pp. 201260.
Zagoskina N.V. Novyye i netraditsionnyye rasteniya i perspektivy ikh ispol'zovaniya. 2018, no. 13, pp. 269273. (in Russ.).
Cheng I.F., Breen K. BioMetals, 2000, vol. 13, no. 3, pp. 7783. DOI: 10.1023/A:1009229429250.
Mee J.J., Jae S.C., Hyun A.J., Ji Y.K., Hae Y.C. Arch. Pharm. Res., 2003, vol. 26, no. 10, pp. 809815.
Dorofeyev V.Yu., Karnachuk R.A., Shilova I.V. Vestnik Tomskogo gosudarstvennogo universiteta. Biologiya, 2008, no. 2(3), pp. 1217. (in Russ.).
Matkowski A. Biotechnology Advances, 2008, vol. 26, no. 6, pp. 548560. DOI: 10.1016/j.biotechadv.2008.07.001.
Tadhani M., Patel V. Subhash R. J. Food Compos. Analysis, 2007, vol. 27(3), pp. 323329. DOI: 10.1016/j.jfca.2006.08.004.
Tikhomirova L.I., Bazarnova N.G., Sysoyeva A.V. Khimiya Rastitel'nogo Syr'ya, 2018, no. 1, pp. 145154. DOI: 10.14258/jcprm.2018012734. (in Russ.).
Tikhomirova L.I., Bazarnova N.G., Il'icheva T.N., Martirosyan YU.TS., Afanasenkova I.V. Khimiya Rastitel'nogo Syr'ya, 2018, no. 4, p. 235245. DOI: 10.14258/jcprm.2018043887. (in Russ.).
Tikhomirova L.I., Bazarnova N.G., Sinitsyna A.A. Russian Journal of Bioorganic Chemistry, 2018, vol. 44, no. 7, pp. 860869.
Tikhomirova L.I., Bazarnova N.G., Khalavin I.A. Russian Journal of Bioorganic Chemistry, 2018, vol. 44(7), pp. 893898. DOI: 10.1134/S1068162018070130.
Popov A.I., Dement'yev Yu.N. Vestnik krasnoyarskogo gosudarstvennogo agrarnogo universiteta, 2017, no. 9, pp. 9196. (in Russ.).
Nozdryukhina L.R., Grinkevich N.I. Narusheniye mikroelementnogo obmena i puti yego korrektsii. [Violation of trace elements and ways of its correction]. Moscow, 1980, 280 p. (in Russ.).
Austenfeld F.A. Z. Pflanzenernahr. und Bodenkunde, 1979, vol. 142, no. 6, pp. 769777.
Nemereshina O.N., Gusev N.F., Filippova A.V. Uspekhi sovremennogo yestestvoznaniya, 2013, no. 11, pp. 54–64. (in Russ.).
Murashige T., Skoog F. Physiol. Plant, 2006, vol. 15, no. 4, pp. 473497. DOI: 10.1111/j.1399–3054.1962.tb08052.x.
FR.1.31.2004.00.986 Metodika vypolneniya izmereniy massovoy kontsentratsii tsinka, kadmiya, svintsa i medi v pishchevykh produktakh, prodovol'stvennom syr'ye, kormov i produktov ikh pererabotki, biologicheski aktiv-nykh do-bavkakh k pishchi, biologicheskikh ob"yektakh metodom inversionnoy vol'tamperometrii na analizatorakh tipa TA–Lab (svidetel'stvo ob attestatsii № 31-04/04 ot 26.12.2003 g) [FR.1.31.2004.00.986 Methodology for measuring the mass concentration of zinc, cadmium, lead and copper in food products, food raw materials, feed and processed products, bio-logically active food additives, biological objects by inverse voltammetry on type analyzers TA – Lab (certificate of certi-fication No. 31-04 / 04 of December 26, 2003)]. (in Russ.).
Perel'man A.I. Geokhimiya. [Geochemistry]. Moscow, 1989, 528 p. (in Russ.).
Afanas'yeva L.V. Khimiya Rastitel'nogo Syr'ya, 2016, no. 3, pp. 103108. DOI: 10.14258/jcprm.2016031197. (in Russ.).
Yel'chininova O.A., Rozhdestvenskaya T.A., Chernykh Ye.Yu. Bioraznoobraziye, problemy ekologii Gornogo Altaya i sopredel'nykh regionov: nastoyashcheye, proshloye, budushcheye. Materialy Mezhdunarodnoy konferentsii. [Biodiver-sity, environmental problems of the Altai Mountains and adjacent regions: present, past, future. Materials of the Interna-tional Conference]. Gorno-Altaysk, 2008, pp. 51–55. (in Russ.).
Il'in V.B. Elementnyy khimicheskiy sostav rasteniy. [Elemental chemical composition of plants]. Novosibirsk, 1985, 129 p. (in Russ.).
SanPiN 2.3.2.1078-01. Gigiyenicheskiye trebovaniya k bezopasnosti i pishchevoy tsennosti produktov. [SanPiN 2.3.2.1078-01. Hygienic requirements for safety and nutritional value of products]. Moscow, 2001, 269 p (in Russ.).
Dobrovol'skiy V.V. Pochvovedeniye, 1997, no. 4, pp. 431441. (in Russ.).
Il'in V.B. Tyazholyye metally v sisteme pochva–rasteniye. [Heavy metals in the soil–plant system]. Novosibirsk, 1991, 151 p. (in Russ.).
Sosorova S.B., Merkusheva M.G., Ubugunov L.L. Khimiya Rastitel'nogo Syr'ya, 2016, no. 2, pp. 5359. DOI: 10.14258/jcprm.201602697. (in Russ.).
Gosudarstvennaya farmakopeya RF XIII izdaniya. [The State Pharmacopoeia of the Russian Federation of the XIII edi-tion]. Moscow, 2015, URL: http://femb.ru. (in Russ.).
Misawa M. Adv Biochem Eng Biotechnol. Springer–Verlag, Berlin, 1985, pp. 5988.
Dicosmo F., Misawa M. Biotechnology Advances, 1995, vol. 13(3), pp. 425453.
Ramachandra R.S., Ravinshankar G.A. Biotechnology Advances, 2002, vol. 20, no. 2, pp. 101153. DOI: 10.1016/S0734-9750(02)00007-1.
Chub V.V. Rost i razvitiye rasteniy. [Plant growth and development]. Moscow, 2005, 640 p. (in Russ.).
Copyright (c) 2020 chemistry of plant raw material
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.
