ISOLATION AND PHYSICOCHEMICAL CHARACTERIZATION OF PECTIN FROM NONCONVENTIONAL NATU-RAL RAW MATERIALS
УДК 54:630.8:664
Abstract
Obtaining and studying the functional properties and determining the subsequent purpose in the practical use of pectin substances requires a thorough study of physical and chemical characteristics of the polysaccharide.
By using the acidic hydrolysis method, pectic polysaccharides were isolated from nonconventional raw materials of natural origins - basidial fungus mushrooms Fomes fomentarius, Ganoderma lucidum, Inonotus hispidus, Polýporus squamosus. The polysaccharides isolated were characterized: such as content of free and etherifying carboxyl groups, total -СООН groups, -ОСН3 groups, degree of etherification and by data of the IR-spectroscopy.
The pectic polysaccharides isolated in identical conditions from the basidial mushrooms were powders with colors from light yellow to is light-brown by the appearance, possess sub-acidic taste, have no extraneous smack and a smell. The pectin from Inonotus hispidus was much lighter than other pectins (Fomes fomentarius, Ganoderma lucidum, Polýporus squamosus). The pectin’s isolated were similar to the apple pectin by the organoleptic properties. They can classified as averagely etherified pectins regarding the degree etherification. Etherification degree of the studied pectins was in the range of 50.00–66.67%. The methoxyl groups were ranged between 8.47–11.43%. Content of free carboxylic groups in the range of 2.25–5.40%.
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Komissarenko S.N., Spiridonov V.N. Rastitel'nyye resursy, 1998, vol. 34, no. 1, pp. 111–119. (in Russ.).
Ovodov Yu.S. Bioorganicheskaya khimiya, 1998, vol. 24, no. 7, pp. 483–501. (in Russ.).
Mohnen D. Curr. Opin. Plant Biol., 2008, vol. 11, no. 3, pp. 266–277. DOI. 10.1016/j.pbi.2008.03.006.
Schols H.A., Vierhuis E., Bakx E.J., Voragen A.G.J. Carbohydr. Res., 1995, vol. 275, no. 2, pp. 343–360. DOI: 10.1016/0008-6215(95)00155-M.
Chirkova T.V. Sorosovskiy Obrazovatel'nyy Zhurnal, 1999, no. 10, pp. 22–27. (in Russ.).
Ofitserov Ye.N., Kostin V.I. Uglevody amaranta i ikh prakticheskoye ispol'zovaniye. [Amaranth carbohydrates and their practical use]. Ul'yanovsk, 2001, 180 p. (in Russ.).
Gawkowska D., Cybulska J., Zdunek A. Polymers, 2018, vol. 10, p. 762. DOI: 10.3390/polym10070762.
Yel'kina Ye.A., Shubakov A.A., Ovodov Yu.S. Khimiya rastitel'nogo syr'ya, 2005, no. 4, pp. 53–56. (in Russ.).
Zolotareva A.M., Churkina T.F., Tsybikova D.Ts., Babuyeva Ts.M. Khimiya rastitel'nogo syr'ya, 1998, no. 1, pp. 29–32. (in Russ.).
Sarafanova L.A. Primeneniye pishchevykh dobavok. Tekhnicheskiye rekomendatsii. 6-ye izd., ispr. i dop. [The use of food additives. Technical recommendations. 6th ed., Rev. and add.]. St.-Petersburg, 2005, 200 p. (in Russ.).
Shakhmatov Ye.G., Mikhaylova Ye.A., Makarova Ye.N. Khimiya rastitel'nogo syr'ya, 2015, no. 4, pp. 15–22. DOI: 10.14258/jcprm.201504878. (in Russ.).
Bedouet L., Denys E., Courtois B., Courtois J. Carbohydr. Polym., 2006, vol. 65, pp. 165–173. DOI: 10.1016/j.carbpol.2005.12.041.
Tulinov A.G., Shlyk M.Yu., Lobanov A.Yu., Mikhaylova Ye.A., Shubakov A.A. Agrarnaya Rossiya, 2017, no. 10, pp. 3–6. (in Russ.).
Potiyevskiy E.G., Novikov A.I. Meditsinskiye aspekty primeneniya pektina. [Medical aspects of the use of pectin]. Moscow, 2002, 96 p. (in Russ.).
Popov S.V. Vzaimodeystviye fagotsitov mlekopitayushchikh s polisakharidami rasteniy. [Interaction of mammalian phagocytes with plant polysaccharides]. Syktyvkar, 2002, 110 p. (in Russ.).
Krylova S.G., Yefimova L.A., Zuyeva Ye.P., Khotimchenko M.Yu., Amosova Ye.N., Razina T.G., Lopatina K.A., Khotimchenko Yu.S. Byull. eksp. biol. med., 2008, vol. 145, pp. 678–681. (in Russ.).
Olano-Martin E., Rimbach G.H., Gibson G.R., Rastall R.A. Anticancer Res., 2003, vol. 23, pp. 341–346.
Sriamornsak P., Sungthongjeen S., Puttipipatkhachom S. Carbohydr. Polym., 2007, vol. 67, pp. 436–445. DOI:10.1016/j.carbpol.2006.06.013. ISSN: 0144-8617.
Nangia-Makker P., Conclin I., Hogan V., Raz A. J. Natl. Cancer Inst., 2002, vol. 94, pp. 1854–1862. DOI: 10.1093/jnci/94.24.1854.
Eliaz I. Clin. Praet. Altern. Med., 2002, vol. 2, pp. 177–179. DOI: 10.1016/j.carres.2008.08.038.
Liu L.S., Fishman M.L., Kost J., Hicks K.B. Biomaterials, 2003, vol. 24, pp. 3333–3343. DOI: 10.1016/s0142-9612(03)00213-8.
Shmidgall J., Hensel A. Internal. J. Biol. Macromol., 2002, vol. 30, pp. 217–225. DOI: 10.1016/S0141-8130(02)00036-3.
Nikitina I.S., Gaynanova L.T., Abdulin M.I., Bespalova A.A. Khimiya rastitel'nogo syr'ya, 2012, no. 2, pp. 21–26. (in Russ.).
Buzyna, H.V., Kybryk É.D., Parfenenko V.V. Proyzvodstvo sveklovychnoho pektyna. [Production of beet pectin]. Moscow, 1974, pp. 1–26. (in Russ.).
Ovodov Yu.S. Bioorganicheskaya khimiya, 2009, vol. 35, no. 3, pp. 293–310. (in Russ.).
Ivanova N.V., Ostroukhova L.A., Babkin V.A. i dr. Khimiya rastitel'nogo syr'ya, 1999, no. 4, pp. 5–7. (in Russ.).
Ovodova R.G., Golovchenko V.V., Popov S.V., Ovodov Yu.S. Izvestiya Komi nauchnogo tsentra UrO RAN, 2010, no. 50, pp. 59–65. (in Russ.).
Patova O.A., Golovchenko V.V., Ovodov Yu.S. Izvestiya AN. Seriya khimicheskaya, 2014, no. 9, pp. 1901–1925. (in Russ.).
Round A.N., MacDougal A.J., Ring S.G., Morris V.J. Carbohydr. Res., 1997, vol. 303, pp. 251–253. DOI: 10.1016/S0008-6215(97)00175-4.
Yefremov A.A., Kondratyuk T.A. Khimiya rastitel'nogo syr'ya, 2008, no. 4, pp. 171–176. (in Russ.).
Aver'yanova Ye.V., Shkol'nikova M.N. Pektin: metody vydeleniya i svoystva. [Pectin: isolation methods and proper-ties]. Biysk, 2015, pp. 34–35. (in Russ.).
Bodyakina I.M., Bagryantsev V.A., Kotov V.V., Lukin A.L. Vestnik VGU, seriya: Khimiya. Biologiya. Farmatsiya, 2012, no. 2, pp. 9–13. (in Russ.).
Ivanova N.V., Popova O.V., Babakin V.A. Khimiya rastitel'nogo syr'ya, 2003, no. 4, pp. 43–46. (in Russ.).
Filippov M.P. Infrakrasnyye spektry pektinovykh veshchestv. [Infrared spectra of pectin substances]. Kishinev, 1978, 74 p. (in Russ.).
Minzanova S.T., Mironov V.F., Konovalov A.I., Vyshtakalyuk A.B., Tsepayeva O.V., Mindubayev A.Z., Mironova L.G., Zobov V.V. Pektiny iz netraditsionnykh istochnikov: tekhnologiya, struktura, svoystva i biologicheskaya aktivnost'. [Pectins from non-traditional sources: technology, structure, properties and biological activi-ty]. Kazan', 2011, pp. 62–64. (in Russ.).
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