Keywords
extraction
supercritical fluid
biologically active substances
How to Cite
Abstract
Natural substances of plant origin are widely used in pharmacies. However, the quantitative content of biologically active substances in plant cells is insignificant and the currently available extraction methods do not allow for the complete extraction of biologically active substances as a result of the strong lignocellulose matrix of the cell. Therefore, it is necessary to look for new extraction methods that increase the yield of extractive substances from plant raw materials. This paper is devoted to the effect of supercritical fluid extraction on the quantitative content of extractives contained in a plant cell. The study aims to establish the effect of supercritical fluid extraction on the lignocellulose matrix and the yield of extractive substances. The plant biomass of the vegetative part of the narrow-leaved cypress (Chamaenerion angustifolium L., Scop., 1771); roots and rhizomes of the stinky bug (Actaea cimicifuga Schipcz., J Compton); roots and rhizomes of the golden root (Rhodiola rosea L., 1753) were used as research objects. The extractive substances were extracted by sequential liquid extraction, supercritical fluid extraction, and a combination of these methods. To extract the maximum amount of extractives, the following solvents were used: a nonpolar solvent (hexane, 70% ethanol) and a highly polar solvent (water). Extraction of plant materials with supercritical CO₂ was carried out in a SFEU-5/2 laboratory setup with the following parameters: pressure (P), 350 bar; temperature (T), 60 °C; extraction time (t), 180 min. The comparison of the yield of extractive substances obtained by sequential liquid extraction and aqueous alcoholic extraction with the preliminary processing of raw materials with supercritical CO₂ shows that treatment with supercritical carbon dioxide activates the plant matrix; therefore, the content of the extracted components increases.
References
Baldino L, Scognamiglio M, Reverchon E (2020) Elimination of tryptamines from green coffee by supercritical CO₂ extraction. Canadian Journal of Chemical Engineering 99: 1345–1351. https://doi.org/10.1002/cjce.23928
Cvjetko Bubalo M, Vidović S, Radojčić Redovniković I, Jokić S (2015) Green solvents for green technologies. Journal of Chemical Technology and Biotechnology 90: 1631–1639. https://doi.org/10.1002/jctb.4668
Dashtianeh M, Vatanara A, Rezazadeh Sh (2018) Supercritical carbon dioxide; An efficient solvent for herbal extraction. Journal of Medicinal Plants 64: 1–11.
Doronin AF, Ipatova LG, Kochetkova AA, Nechaev AP, Shubina OG, Khurshudyan SA (2008) Functional Foodstuff: The Textbook. DeLi print, Moscow, 288 p. [In Russian]
Gumerov F, Yarullin R (2008) Supercritical fluids and SCF technologies. The Chemical Journal 1: 26–30. [In Russian]
Hu F, Ragauskas A (2012) Pretreatment and lignocellulosic chemistry. Bioenergy Research 5: 1043–1066. https://doi.org/10.1007/s12155-012-9208-0
Huang Z, Shi X, Jiang W (2012) Theoretical models for supercritical fluid extraction. Journal of Chromatography A 1250: 2–26. https://doi.org/10.1016/j.chroma.2012.04.032
Newman DJ, Cragg GM, Snader KM (2003) Natural products as sources of new drugs over the period 1981-2002. Journal of Natural Products 66: 1022–1037. https://doi.org/10.1021/np030096l
Osborne J (2014) Handbook on Supercritical Fluids: Fundamentals, Properties and Applications. Nova Science Publisher, New York, 407 p.
Raskin I, Ripoll C (2004) Can an apple a day keep the doctor away? Current Pharmaceutical Design 10: 3419–3429. http://dx.doi.org/10.2174/1381612043383070
Sidelnikov VN, Patrushev YuV, Sizova NV et al. (2003) Comparative analysis of the composition of fir oil obtained by water-steam distillation and the essential oil fraction of the CO₂-extract of the Siberian fir paw. Chemistry of Vegetable Raw Material 1: 79–85. [In Russian]
Sovová H (1994) Rate of the vegetable oil extraction with supercritical CO₂–I. Modeling of extraction curves. Chemical Engineering Science 49: 409–414. [In Russian]
Vagi E, Simandi B, Suhajda A, Héthelyi EB (2005) Essential oil composition and antimicrobial activity of Origanum majorana L. extracts obtained with ethyl alcohol and supercritical carbon dioxide. Food Research International 38: 51–57. http://dx.doi.org/10.1016/j.foodres.2004.07.006
Wijngaard H, Hossain MB, Rai DK, Brunton N (2012) Techniques to extract bioactive compounds from food by-products of plant origin. Food Research International 46: 505–513. https://doi.org/10.1016/j.foodres.2011.09.027
Zhang J, Wen C, Zhang H, Duan Y, Ma H (2019) Recent advances in the extraction of bioactive compounds with subcritical water: A review. Trends in Food Science and Technology 95: 183–195. https://doi.org/10.1016/j.tifs.2019.11.018
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