Analysis of genetic diversity among Iranian populations of Ficus carica L. (Moraceae)
PDF
XML

Keywords

RAPD
Ficus carica
population
genetic diversity

How to Cite

Zolfaghari, M., Salimpour, F., Sharifnia, F., & Marandi, S. (2019). Analysis of genetic diversity among Iranian populations of Ficus carica L. (Moraceae). Acta Biologica Sibirica, 5(3), 131-138. https://doi.org/10.14258/abs.v5.i3.6534

Abstract

Ficus carica L. is a species of Moraceae family that has been cultivated in different parts of the world from ancient time. Its fruit is eaten by human and has several medicinal properties. This species widely grows and cultivated in various parts of Iran. For this, in the current study, we investigated infraspecific genetic variation and population structure of F. carica in the country. 14 natural populations of F. carica were collected and their genomic DNA were extracted and tested with RAPD molecular technique. Parameters of population’s genetic diversity varied among the studied populations. In addition, AMOVA test revealed significant variations among the populations.  The studied populations clustered separately in UPGMA tree of RAPD data, moreover PCA and MDS plots produced similar results. STRUCTURE analysis revealed the best number of k = 9. Based on Nei’s genetic distance, we had nine distinct groups. Genetic clustering patterns according to UPGMA trees of RAPD data and Nei’s genetic distance were more similar with the results of STRUCTURE analysis. The small amount of Nm) the product of the effective size of individual populations (N) and the rate of migration among them (m) (value showed limited gene flow among the studied populations, therefore it seems that high genetic variations among these populations may be related less or few amount of gene exchange among populations.

https://doi.org/10.14258/abs.v5.i3.6534
PDF
XML

References

Aksoy, U., Can, H.Z., Misirli, A., Kara, S., Seferoglu, G., Sahin, N. (2003). Fig (Ficus carica L.) selection study for fresh market in Western Turkey. Acta Horticulturae, 605, 197-203. https://doi.org/10.17660/ActaHortic.2003.605.29

Achtak, H., Oukabli, A., Ater, M., Santoni, S., Kjellberg, F., Khadari, B. (2009). Microsatellite Markers as Reliable Tools for Fig Cultivar Identification. Journal of American Society for Horticultural Science, 134, 624-631. https://doi.org/10.21273/JASHS.134.6.624

Basheer-salimia, R., Awad, M., Salama, A., Alseek, S., Harb, J., Hamden, Y. (2012). Molecular Polymorphisms in Palestinian Figs (Ficus carica L.) as Revealed by Random Amplified Polymorphic DNA (RAPD). Journal of Genetic Engineering and Biotechnology, 10, 169-175. https://doi.org/10.1016/j.jgeb.2012.07.001

Baziar, Gh., Jafari, M., Sharifi-Noori, M. S., Samarfard, S. (2018). Evaluation of Genetic Diversity among Persian Fig Cultivars by Morphological Traits and RAPD Markers. Hortscience, 53(5), 613-619. https://doi.org/10.21273/HORTSCI11306-16

Beck, N. G., Lord, E. M. (1988). Breeding system in Ficus carica, the common fig. I. Floral diversity. American Journal of Botany, 75, 1904-1912. https://doi.org/10.1002/j.1537-2197.1988.tb11271.x

Berg, C. C. (1989). Classification and distribution of Ficus. Experientia, 45, 605-611. https://doi.org/10.1007/BF01975677

Dalkılıç, Z., Mesav, H.O., Günver-Dalkılıç, G., Kocataş, H. (2011). Genetic diversity of male fig (Ficus carica caprificusL.) genotypes with random amplified polymorphic DNA (RAPD) markers. African Journal of Biotechnology, 10, 519-526.

Esquinas Alcazar, J. (2005). Protecting crop genetic diversity for food security: political, ethical and technical challenges. Nature Reviews Genetics, 6, 946-953. https://doi.org/10.1038/nrg1729

Evanno, G., Regnaut, S., Goudet, J. (2005). Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Molecular Ecology, 14(8), 2611-2620. https://doi.org/10.1111/j.1365-294X.2005.02553.x

F.A.O. (2011). Stat Agricultural Data. Available from: http://faostat.ao.org/.

Falistocco, E., Antonielli, L. (2002). Molecular cytogenetics of Vitis vignifera L. and Ficus carica L.: location of rDNA sequences. Proc. XLVI Ital. Soc. Agric. Genet. SIGA Ann. Congr. Giardini, Nexos, Italy.

Falush, D., Stephens, M., Pritchard, J.K., (2007). Inference of population structure using multilocus genotype data: dominant markers and null alleles. Molecular Ecology Notes, 7(4), 574-578. https://doi.org/10.1111/j.1471-8286.2007.01758.x

Freeland, J.R., Kirk, H., Peterson, S.D. (2011). Molecular Ecology, 2nd ed., UK: Wiley−Blackwell. https://doi.org/10.1002/9780470979365

Fu, Y.B. 2015. Understanding crop genetic diversity undermodern plant breeding. Theoretical and Applied Genetics, 128, 2131-2142. https://doi.org/10.1007/s00122-015-2585-y

Hamrick, J. L., Godt, M. J. W. (1996). Conservation genetics of endemic plant species (pp. 281-304). In: Avise, J. C., Hamrick, J. L. (Eds), Conservation genetics. Chapman and Hall. https://doi.org/10.1007/978-1-4757-2504-9_9

Hedrick, P.W. (2005). A standardized genetic differentiation measure. Evolution, 59(8), 1633-1638. https://doi.org/10.1111/j.0014-3820.2005.tb01814.x

Huson, D.H., Bryant, D. (2006). Application of phylogenetic networks in evolutionary studies. Molecular Biology and Evolution, 23(2), 254-267. https://doi.org/10.1093/molbev/msj030

Kislev, E. M., Hartman, A., Bar‐Yosef, O. (2006). Early domesticated fig in the Jordan Valley. Science, 312, 1372-1374. https://doi.org/10.1126/science.1125910

Kocsis, M., Jaromi, L., Putnoky, P., Kozma, P. and Borhidi, A. (2005). Genetic Diversity among Twelve Grape Cultivars Indigenous to the Carpathian Basin Revealed by RAPD Markers. Vitis, 44, 87-91.

Mars, M. (2003). Conservation of fig (Ficus carica L.) and pomegrenate (Prunica granatum L.) varieties in Tunisia (pp. 433-442). In: Lemons, J., Victor, R., Schaffer, D. (Eds.), Conserving biodiversity in arid regions, Kluwer. https://doi.org/10.1007/978-1-4615-0375-0_33

Meirmans, P.G. (2012). AMOVA−based clustering of population genetic data, Journal of Heredity, 103(5), 744-750. https://doi.org/10.1093/jhered/ess047

Meirmans, P.G., Van Tienderen, P.H. (2004). GENOTYPE and GENODIVE: two programs for the analysis of genetic diversity of asexual organisms. Molecular Ecology Notes, 4, 792-794. https://doi.org/10.1111/j.1471-8286.2004.00770.x

Moritz, C. (1994). Defining Evolutionary Significant Units for Conservation. Trends in Ecology and Evolution, 9, 373- 375. https://doi.org/10.1016/0169-5347(94)90057-4

Peakall, R., Smouse, P.E. (2006). GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes, 6, 288-295. https://doi.org/10.1111/j.1471-8286.2005.01155.x

Pritchard, J.K., Stephens, M., Donnelly, P. (2000). Inference of population structure using multilocus genotype data. Genetics, 155(2), 945-959.

Sadder, M.T., Atteyyeh, A.F. (2006). Molecular Assessment of Polymorphism among Local Jordanian Genotypes of The Common Fig (Ficus carica L.). Scientia Horticulturae, 107, 347-351. https://doi.org/10.1016/j.scienta.2005.11.006

Salhi-Hannachi, A., Chatti, K., Saddoud, O., Mars, M., Rhouma, A., Marrakchi, M., Trifi, M. (2006). Genetic Diversity of Different Tunisian Fig (Ficus carica L.) Collection Revealed by RAPD Fingerprints. Hereditas, 143, 15-22. https://doi.org/10.1111/j.2005.0018-0661.01904.x

Salhi‐Hannachi, A., Chatti, K., Mars, M. et al. (2005). Comparative analysis of genetic diversity in two Tunisian collections of fig cultivars based on random amplified polymorphic DNA and inter simple sequence repeats fingerprints. Genetic Resources and Crop Evolution, 52, 563-573. https://doi.org/10.1007/s10722-003-6096-3

Şimşek, M., Yildirim, H. (2010). Fruit characteristics of the selected fig genotypes. African Journal of Biotechnology, 9, 6056-6060.

Sheidai, M., Ziaee, S., Farahani, F., Talebi, S.M., Noormohammadi, Z. & Hasheminejad Ahangarani Farahani, Y. (2014). Infra-specific genetic and morphological diversity in Linum album (Linaceae). Biologia, 69, 32-39. https://doi.org/10.2478/s11756-013-0281-4

Valdeyron, G., Lloyd, D. G. (1979). Sex differences and flowering phenoloy in the common fig (Ficus carica L.). Evolution, 33, 673-685. https://doi.org/10.1111/j.1558-5646.1979.tb04720.x

Watson, L., Dallwitz, M.J. (2004). The families of flowering plants: descriptions, illustrations, identification and information retrieval. Available from: http://delta-intkey.com/ (Accessed 20.05. 2010).

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...