Аннотация
Hop (Humulus lupulus L.) is a perennial crop propagated vegetatively. There are a number of problems associated with preserving the gene pool, obtaining certified planting material, and replenishing the collection of hop varieties. To solve these problems, it is promising to use biotechnological methods and attract genetic resources from natural flora. The article presents the results of an experimental research on introducing into tissue culture and studying the effect of cytokinin growth regulators on in vitro reproduction of wild hop genotypes from the Altai Krai. A comparison with the in vitro morphogenesis of varieties bred in Russia is provided. Wild hop samples have been shown to be successfully introduced into tissue culture at a level not lower than that of national varieties and are characterized by intensive growth of shoots and root systems in hormone-free Murashige and Skoog (MS) medium. The ANOVA results confirmed that the genetic characteristics of the samples had the greatest impact on the reproduction rate and height of hop plants in vitro culture. The second most important factor was the type of plant growth regulator. The maximum height of the shoots of the ‘Flagman’ variety and the wild sample AK-3 was in the MS nutrient medium containing 5 μM kinetin, and for the ‘Civil'skij’ variety – 1 μM 6-benzylaminopurine. For the AK-1 genotype, the best results were obtained in hormone-free MS medium. The reproduction rate, calculated as the ratio of the the height of shoots, including the lateral ones, to the number of internodes, ranged from 3.9 to 9.1. For wild samples, it was possible to achieve reproduction rates of 8–9 in one passage lasting 8 weeks. The results confirmed the high morphogenetic potential of wild hop samples AK-1 and AK-3 from the south of Western Siberia, as well as the national variety ‘Flagman’ for the use of clonal micropropagation technology to preserve genotypes in the in vitro collection, breeding, and production of planting material.
Литература
Bedir H, Ari E, Vural GE, Seguí-Simarro JM (2022) Effect of the genotype, explant source and culture medium in somatic embryogenesis and organogenesis in Vaccariah ispanica (Mill.) Rauschert. Plant Cell, Tissue and Organ Culture 150: 329-343. https://doi.org/10.1007/s11240-022-02275-8
Demidchik V, Chernysh M, Ditchenko D, Spiridovich E, Przhevalskaya D, Padutov V (2019) Microclonal propagation of plants. Science and innovation 6 (196): 4-11. [In Russian]
de-Souza R, Adams C, Melo R, Guidolin A, Michel A, Coimbra J (2022) Growth regulators and their reflection on different hop genotypes cultivated under in vitro conditions. Brazilian Journal of Biology 82: e242596. https://doi.org/10.1590/1519-6984.242596
FAO 2014 Genebank standards for plant genetic resources for food and agriculture. Rev. ed. Rome. Available from: https://openknowledge.fao.org/items/d5af34e2-7abc-415d-9bee-28802479d44f
Gashenko OA, Kastritskaya MS, Kukharchik NV (2019) Micropropagation of hop varieties in in vitro culture. Subtropical and ornamental gardening 68: 111-118. [In Russian]
Girichev VS, Vysotsky LA, Marchenko LV, Alekseenko AA, Danilova AV, Artyukhova VA (2012) Collections of fruit, berry and ornamental plants as a tool for increasing the efficiency of the breeding process. Agricultural Biology 5: 48–53. [In Russian]
Hirakawa T, Tanno S (2022) In vitro propagation of Humulus lupulus through the induction of axillary bud development. Plants 11: 1066. https://doi.org/10.3390/plants11081066
Iacuzzi N, Salamone F, Farruggia D, Tortorici N, Vultaggio L, Tuttolomondo T (2023) Development of a new micropropagation protocol and transfer of in vitro plants to in vivo conditions for Cascade hop. Plants 12: 2877. https://doi.org/10.3390/plants12152877
Kastritskaya MS, Kukharchik NV, Gashenko OA (2014) Micropropagation of hop varieties in vitro. Vestnik Nacional'noj Akademii Navuk Belarusi. Ser. Agrar. Navuk 2: 75–80. [In Russian]
Khlynovskiy MD, Mironenko ON, Khlebova LP, Bychkova OV, Brovko ES, Nebylitsa AV (2023) Actual issues of hop growing in Russia. Proceed. Science-Technical Commission of the International Hop Growers’ Convention, 25-29 June 2023, Ljubljana, Slovenia. Wolnsach: Druckhaus Kastner AG. Pp. 85-89.
Korpelainen H, Pietiläinen M (2021) Hop (Humulus lupulus L.): Traditional and present use, and future potential. Economic Botany 75(3): 302–322. https://doi.org/10.1007/s12231-021-09528-1
Kosenko IS, Koldar LA, Denysko IL, Balabak OA, Nebykov MV, Balabak AF, Balabak AV (2021) Morphogen development of in vitro cultivated Shrub roses. Ukrainian Journal of Ecology 11(2): 229–235. https://doi.org/10.15421/2021_104
Krahmaleva IL, Molkanova OI, Malaeva EV (2019) Features of clonal micropropagation of different forms of promising varieties Actinidia kolomikta (Rupr. Etmaxim) Maxim. Bulletin of the State Nikitsky Botanical Garden 133: 80–86. https://doi.org/10.36305/0513-1634-2019-133-80-86. [In Russian]
Lata H, Chandra S, Khan IA, Elsohly MA (2009) Propagation through alginate encapsulation of axillary buds of Cannabis sativa L. – an important medicinal plant. Physiol. Mol. Biol. Plants 15(1): 79-86. https://doi.org/10.1007/s12298-009-0008-8
Lutova L Dodueva I (2019) Genetic control of regeneration processes of radish plants in vitro: from phenotype to genotype. Biological Communications 64(2): 124-132. https://doi.org/10.21638/spbu03.2019.204
Machado MP, Gomes EN, Francisco F, Bernert AF, Bespalhok Filho JC, Deschamps C (2018) Micropropagation and establishment of Humulus lupulus L. plantlets under field conditions at southern Brazil. The Journal of Agricultural Science 10 (7): 275-281. http://dx.doi. org/10.5539/jas.v10n7p275
Mitrofanova IV, Ivanova NN, Zhdanova IV (2018) In vitro deposition of ornamental, aromatic and fruit plants. In: IV Mitrofanova (ed.). Fundamentals of creating an in vitro gene bank of species, varieties and forms of ornamental, aromatic and fruit crops. Simferopol: IT “ARIAL” Pp. 171-256. [In Russian]
Molokanova OI, Koroleva OV, Stakheeva TS, Krakhmaleva IL, Meleshchuk EA (2018) Improving the technology of clonal micropropagation of valuable fruit and berry crops for production conditions. Achievement of science and technology of the agro-industrial complex 32(9): 66–69. [In Russian]
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum 15 (3): 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Nikonova ZA, Korotkova ZP (2017) Creation and preservation of a collection of common hops as a gene pool for breeding. Niva Povolzhye 4(45): 104-108. [In Russian]
Panis B, Nagel M, den Houwe IV (2020) Challenges and prospects for the conservation of crop genetic resources in field genebanks, in in vitro collections and/or in liquid nitrogen. Plants 9 (12): 1634. https://doi.org/10.3390/plants9121634
Pereira O, Santos G, Sousa M. (2022). Hop by-products: pharmacological activities and potential application as cosmetics. Cosmetics 9(6): 139. https://doi.org/10.3390/cosmetics9060139
Pua EC, Gong H (2004) Regulation of plant morphogenesis in vitro. In: Pua EC, Douglas CJ (eds) Brassica. Biotechnology in Agriculture and Forestry, 54. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-06164-0_6
Rajasekharan PE, Sahijram L (2015) In vitro conservation of plant germplasm. In B. Bahadur et al. (eds.), Plant Biology and Biotechnology: Volume II: Plant Genomics and Biotechnology, Springer India. https://doi.org/10.1007/978-81-322-2283-5_22
Romanov GA, Medvedev SS (2006) Auxins and cytokinins in plant development. Recent advances in phytohormone research. Physiology of plants 53 (2): 309–319. [In Russian]
Roy AT, Leggett G, Koutoulis A (2001) Development of a shoot multiplication system for hop (Humulus lupulus L.). In Vitro Cell. Dev. Biol. – Plant 37: 79-83. https://doi.org/10.1007/s11627-001-0015-0
Shimizu-Sato S, Tanaka M, Mori H. (2009) Auxin-cytokinin interactions in the control of shoot branching. Plant Mol. Biol. 69(4): 429–435. https://doi.org/10.1007/s11103-008-9416-3
Shulgina AA, Kalashnikova EA, Tarakanov IG, Kirakosyan RN, Cherednichenko MYu, Polivanova OB, Baranova EN, Khaliluev MR (2021) Influence of light conditions and medium composition on morphophysiological characteristics of Stevia rebaudiana Bertoni in vitro and in vivo. Horticulturae 7(7): 195. https://doi.org/10.3390/horticulturae7070195
Sugla T, Purkayastha J, Singh SK, Solleti SK (2007) Micropropagation of Pongamia pinnata through enhanced axillary branching. In Vitro Cell. Dev. Biol. – Plant 43(5): 409–414. https://doi.org/10.1007/s11627-007-9086-x
Tyagi RK, Grawal AA (2015) Revised genebank standards for management of plant genetic resources. Indian Journal of Agricultural Sciences 85(2): 157–165. https://doi.org/10.56093/ijas.v85i2.46437
Yamaguchi I, Cohen JD, Culler AH, Quint M, Slovin JP, Nakajima M, Sakagami Y (2010) Plant hormones. In: Comprehensive Natural Products II Chemistry and Biology, Oxford: Elsevier Рp. 9-125. https://doi.org/10.1016/b978-008045382-8.00092-7
Это произведение доступно по лицензии Creative Commons «Attribution» («Атрибуция») 4.0 Всемирная.