FLAVONOIDS AND LIGNANS FROM THE LEAVES OF NEPETA MULTIFIDA (LAMIACEAE) AND THEIR BIOAC-TIVITY

UDC 582.949.27+547.9+543.544.5.068.7

  • Nina Igorevna Kashchenko Institute of General and Experimental Biology, Siberian Branch, Russian Academy of Science Email: ninkk@mail.ru
  • Daniil Nikolayevich Olennikov Institute of General and Experimental Biology, Siberian Branch, Russian Academy of Science http://orcid.org/0000-0001-8194-1061 Email: olennikovdn@mail.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.20220210569
Keywords: Nepeta multifida, Schizonepeta, Lamiaceae, flavonoids, luteolin, rosmarinic acid, HPLC-UV, acetylcholinesterase

Abstract

A perennial plant Nepeta multifida L. (syn. Schizonepeta multifida (L.) Briq.) is one of the most common species of the Lamiaceae family growing in Eastern Siberia and used in traditional oriental medicine. The chemical composition of N. multifida has not been sufficiently studied. Chromatographic separation of phenolic compounds of N. multifida leaves using column chromatography and preparative HPLC resulted in the isolation of sixteen compounds, including a new flavonoid identified as luteolin-7-O-(3′′,6′′-di-O-acetyl)-β-D-glucopyranoside. Known compounds were O-glycosides of luteolin and apigenin, rosmarinic acid, salvianolic acids A and B, and schizotenuin A. Quantitative analysis of N. multifida leaves by HPLC-UV assay showed the high content of rosmarinic acid (8.36–35.71 mg/g), luteolin-7-O-glucuronide (2.03–14.18 mg/g) and schizotenuin A (5.29–9.56 mg/g). The highest level of phenolic compounds was found in the flowering and fruiting phases. Using Ellman’s spectrophotometric method, it was found that N. multifida leaf extract and some compounds had antiacetylcholinesterase activity, and luteolin glycosides being the most active showed the level of concentration of half-maximal enzyme inhibition (IC50) 29.03–58.36 μg/mL. Thus, as a result of the present study, it was found that the leaves of N. multifida contain various groups of phenolic compounds capable of inhibiting the activity of acetylcholinesterase.

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Author Biographies

Nina Igorevna Kashchenko, Institute of General and Experimental Biology, Siberian Branch, Russian Academy of Science

кандидат фармацевтических наук, старший научный сотрудник лаборатории медико-биологических исследований

Daniil Nikolayevich Olennikov, Institute of General and Experimental Biology, Siberian Branch, Russian Academy of Science

доктор фармацевтических наук,  заведующий лабораторией медико-биологических исследований

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Published
2022-06-10
How to Cite
1. Kashchenko N. I., Olennikov D. N. FLAVONOIDS AND LIGNANS FROM THE LEAVES OF NEPETA MULTIFIDA (LAMIACEAE) AND THEIR BIOAC-TIVITY // chemistry of plant raw material, 2022. № 2. P. 85-94. URL: http://journal.asu.ru/cw/article/view/10569.
Issue
No 2 (2022)
Section
Low-molecular weight compounds

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