PHARMACOGNOSTIC STUDY OF COSMOS BIPINNATUS CAV. (FAM. ASTERACEАE), CULTURED IN THE WESTERN CAUCASIAN REGION
UDC 615.322. 615.072. 547
Abstract
The purpose of the work: to reveal the study of the morpho-anatomical composition and composition of biologically active groups – compounds, carboxylic acids and trace elements of the aerial part of various species of Cosmos bipinnatus Cav.: ‘Purity’, ‘Rosea’ and ‘Dazzler’.
Materials and methods. The object of the study is the cultivation of three species of space plants in Europe, collected in the flowering phase at the beginning of 2020. Micropreparations were obtained using a Biomed-2 microscope (eyepiece 16x, objective 4x, 10x) in accordance with the procedures of the State Pharmacopoeia of the Russian Federation, XVI edition (SP XVI). Carbohydrate complexes are isolated from dried raw materials by the method of N.K. Kochetkov and M. Sinner. Quantify the content of each assessment gravimetrically. The monosaccharide composition is established after acid hydrolysis by paper chromatography. Identification of acid consumption is carried out using thin layer chromatography. The elemental and amino acid composition is examined by capillary electrophoresis.
Results and discussion. Certain characteristic microscopic features of Cosmos bipinnatus Cav., Aster varieties ‘Rosea’, ‘Dazzler’, ‘Purity’ for various morphological parts of plants (leaves, flowers, inflorescence involucre, stem), revealing the identified signs of the alleged medicinal source as a source of biologically active connections. A comparative analysis of the composition of carbohydrates, carboxylic acids and carbohydrates of shoots (leaves and stems) showed that water-soluble polysaccharides are arabinose fructose, rhamnose and galactose, pectin monosaccharides are galacturonic acid, glucose and arabinose detection, and hemicelluloses A and B are xylose, glucose and galactose. In all known varieties, succinic acid is noted. A high content of proline and essential amino acids: leucine, methionine, valine, threonine, as well as the ability of all the studied sources to collect potassium, magnesium, iron and boron ions were noted.
Conclusion. It has been established that the unusual colors of the flowers are predominantly the color of the stems. The anatomical features of different species are practically the same. It was revealed that the ‘Purity’ variety surpasses the objects in terms of the content of water-soluble polysaccharides, cellulose-hemi A and B, other essential amino acids, aliphatic amino acids, iron and boron. Variety ‘Dazzler’ is the richest in pectin doses and potassium ions. ‘Rosea’ variety contains the maximum amount of high content of amino acids.
Downloads
Metrics
References
Masyukova M.A., Sushkov K.L. Nashi tsvety. [Our flowers]. Alma-Ata, 1972, 235 p. (in Russ.).
Buschhaus Ch., Peng Ch., Jetter R. Phytochemistry, 2013, vol. 91, pp. 249–256.
Zhizn' rasteniy. Ch. 1. [Plant life. Part 1] / ed. A.L. Takhtajyan. Moscow, 1980, vol. 5, 430 p. (in Russ.).
Olajuyigbe O., Ashafa A. Iranian Journal of Pharmaceutical Research, 2014, vol. 13(4), pp. 1417–1423.
Menut C., Bessiere J.M., Zollo P.A., Kuiate J.R. Journal Essential Oil-Bearing Plants, 2000, vol. 3, pp. 65–69.
Saito K. Zeitschrift Für Pflanzenphysiologie, 1974, vol. 71(1), pp. 80–82. DOI: 10.1016/s0044-328x(74)80190-x.
Bate-Smith E.C. Phytochemistry, 1980, vol. 19, p. 982. DOI: 10.1016/0305-1978(93)90096-A.
Akihisa T., Yasukawa K., Oinuma H., Kasahara Y., Yamanouchi S., Takido M., Kumaki K., Tamura T. Phytoche-mistry, 1996, vol. 43(6), pp. 1255–1260. DOI: 10.1016/s0031-9422(96)00343-3.
Gosudarstvennaya farmakopeya Rossiyskoy Federatsii, XVI izd. [State Pharmacopoeia of the Russian Federation, XVI ed.]. Moscow, 2018. URL: http://www.femb.ru/feml. (in Russ.).
Kochetkov N.K., Bochkov A.F., Dmitriyev B.A. et al. Khimiya uglevodov. [Chemistry of carbohydrates]. Moscow, 1967, 672 p. (in Russ.).
Anan'ina N.A., Andreyeva O.A., Oganesyan E.T. Khimiya rastitel'nogo syr'ya, 2008, no. 2, pp. 135–136. (in Russ.).
Butenko L.I., Ligay L.V., Podgornaya Zh.V. Mezhdunarodnyy zhurnal eksperimental'nogo obrazovaniya, 2016, no. 10, pp. 193–195. (in Russ.).
Kochetkov N.K. Khimiya biologicheski aktivnykh veshchestv. [Chemistry of biologically active substances]. Moscow, 1970, 631 p. (in Russ.).
Amoyan E.F., D'yakova D.A., Tkachenko A.V. Mezhdunarodnyy studencheskiy nauchnyy vestnik, 2016, no. 3-3, pp. 436–437. (in Russ.).
Doson R., Eliot D., Dzhons K. Spravochnik biokhimika. [Biochemist's Handbook]. Moscow, 1991, 544 p. (in Russ.).
Gosudarstvennaya Farmakopeya SSSR. XI izd. [State Pharmacopoeia of the USSR. XI ed.]. Moscow, 1990, vol. 2, pp. 294–297. (in Russ.).
Komarova N.K. Prakticheskoye rukovodstvo po ispol'zovaniyu sistem kapillyarnogo elektroforeza «Kapel'». [A practi-cal guide to the use of capillary electrophoresis systems «Kapel'»]. St. Petersburg, 2006, 212 p. (in Russ.).
Zakharova M.V., Kiseleva G.K., Lifar' G.V., Yakuba Yu.F. Metodicheskoye i analiticheskoye obespecheniye issledo-vaniy po sadovodstvu. [Methodological and analytical support for research in horticulture]. Krasnodar, 2010, pp. 273. (in Russ.).
Zakharova M.V., Il'ina I.A., Lifar' G.V., Yakuba Yu.F. Metodicheskoye i analiticheskoye obespecheniye issledovaniy po sadovodstvu. [Methodological and analytical support for research in horticulture]. Krasnodar, 2010, pp. 289–295. (in Russ.).
Zakharova M.V., Il'ina I.A., Kiseleva G.K., Lifar' G.V., Yakuba Yu.F. Metodicheskoye i analiticheskoye obespecheniye issledovaniy po sadovodstvu. [Methodological and analytical support for research in horticulture]. Krasnodar, 2010, pp. 273–278. (in Russ.).
Kulichenko Ye.O., Lukashuk S.P., Oganesyan E.T. Yevraziyskiy soyuz uchenykh (YeSU). Euroasia. Meditsinskiye nauki, 2017, no. 10(43), pp. 72–74. (in Russ.).
Kurkin V.A., Avdeeva E.V., Tarasenko L.V., Ryzhov V.M., Shagalieva N.R., Aznagulova A.V., Marlynova L.V. Meditsinskiy al'manakh, 2013, no. 5 (29), pp. 191–196. DOI: 10.21581/2311-3456-2011-5(28)-191-196. (in Russ.).
Sklyarevskaya N.V. Farmakognosticheskoye izucheniye nadzemnoy chasti Sabel'nika bolotnogo (Comarum palustre L.), proizrastayushchego na severo-zapade Rossii: avtoref. dis. … kand. farm. nauk. [Pharmacognostic study of the aerial part of the marsh cinquefoil (Comarum palustre L.), growing in the north-west of Russia: abstract dis. … cand. farm. sciences]. St. Petersburg, 2009, 25 p. (in Russ.).
Ngo Thi Thanh Giep. Farmakognosticheskoye izucheniye travy Leonurus japonicus Houtt.: avtoref. dis. … kand. farm. nauk. [Pharmacognostic study of the herb Leonurus japonicus Houtt.: abstract dis. … cand. farm. sciences]. St. Peters-burg, 2008, 25 p. (in Russ.).
Razarenova K.N. Farmakognosticheskoye izucheniye nekotorykh vidov roda Geranium L.: avtoref. dis. … kand. farm. nauk. [Pharmacognostic study of some species of the genus Geranium L.: abstract dis. … cand. farm. sciences]. St. Pe-tersburg, 2013, 23 p. (in Russ.).
Dayronas Zh.V., Zilfikarov I.N., Vandyshev V.V., Miroshnikova Ye.A. Farmatsiya i farmakologiya, 2017, no. 5(2), pp. 117–134. DOI:10.19163/2307-9266-2017-5-2-117-134. (in Russ.).
Galiakhmetova E.Kh. Razrabotka i standartizatsiya sbora dlya profilaktiki i lecheniya narusheniy mozgovogo krovoobrashcheniya: avtoref. dis. … kand. farm. nauk. [Development and standardization of the collection for the pre-vention and treatment of cerebrovascular accidents: abstract dis. … cand. farm. sciences]. Perm, 2007, 24 p. (in Russ.).
Artem'yeva V.V., Guseva Ye.A. Novyye tekhnologii, 2010, no. 1, pp. 121–124. DOI: 10.21581/2311-3456-2010-1-121-124. (in Russ.).
Kurkina A.V., Tarasenko L.V., Ryzhov V.M. Meditsinskiy al'manakh, 2010, no. 4, pp. 63–66. DOI: 10.21581/2311-3456-2010-4-63-66. (in Russ.).
Mar'in A.A. Razrabotka i standartizatsiya serdechno-sosudistogo sbora: avtoref. dis. … kand. farm. nauk. [Develop-ment and standardization of cardiovascular collection: abstract dis. … cand. farm. sciences]. Tomsk, 2006, 23 p. (in Russ.).
Jackson B.P., Snowdon D.W. Atlas of microscopy of medicinal plants, culinary herbs and spices. London, 1990, 257 p.
Sampiyev A.M., Shevchenko A.I. Kubanskiy nauchnyy meditsinskiy vestnik, 2006, vol. 12, pp. 98–101. DOI: 10.21581/2311-3456-2006-12-98-101. (in Russ.).
European Pharmacopoeia. 8th ed. 2013, 3655 p.
Rastitel'nyye resursy SSSR. T. 7. Sem. Asteraceae. [Plant resources of the USSR. Vol. 7. Fam. Asteraceae]. St. Peters-burg, 1993, 352 p. (in Russ.).
Entsiklopedicheskiy slovar' lekarstvennykh rasteniy i produktov zhivotnogo proiskhozhdeniya: uchebnoye posobiye. [Encyclopedic Dictionary of Medicinal Plants and Animal Products: Study Guide]. St. Petersburg, 2002, 765 p. (in Russ.).
Saleem M., Ali H.A., Akhtar M.F., Saleem U., Saleem A., Irshad I. Natural Product Research, 2019, vol. 33, no. 6, pp. 897–900. DOI: 10.1080/14786419.2017.1413557.
Belonogova V.D. Resursy, ekologicheskaya bezopasnost' i fotokhimicheskiye issledovaniya dikorastushchikh lekar-stvennykh rasteniy Permskogo kraya: avtoref. dis. … kand. farm. nauk. [Resources, environmental safety and photo-chemical studies of wild-growing medicinal plants in the Perm region: abstract dis. … cand. farm. sciences]. Perm, 2009, 39 p. (in Russ.).
Lunina N.M. et al. Dekorativnyye travyanistyye rasteniya kul'turnoy flory Belarusi. [Ornamental herbaceous plants of the cultural flora of Belarus]. Minsk, 2010, 170 p. (in Russ.).
Zernov V.S., Alekseyev Yu.Ye., Onipchenko V.G. Opredelitel' sosudistykh rasteniy Karachayevo-Cherkesskoy Respubliki. [Key to vascular plants of the Karachay-Cherkess Republic]. Moscow, 2015. 454 p. (in Russ.).
Copyright (c) 2023 chemistry of plant raw material
This work is licensed under a Creative Commons Attribution 4.0 International License.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The authors, which are published in this journal, agree to the following conditions:
1. Authors retain the copyright to the work and transfer to the journal the right of the first publication along with the work, at the same time licensing it under the terms of the Creative Commons Attribution License, which allows others to distribute this work with the obligatory indication of the authorship of this work and a link to the original publication in this journal .
2. The authors retain the right to enter into separate, additional contractual agreements for the non-exclusive distribution of the version of the work published by this journal (for example, to place it in the university depository or to publish it in a book), with reference to the original publication in this journal.
3. Authors are allowed to post their work on the Internet (for example, in a university repository or on their personal website) before and during the review process of this journal, as this may lead to a productive discussion, as well as more links to this published work.
