IDENTIFICATION OF MARKERS FOR MYCELIA OF WHITE-ROT AND BROWN-ROT FUNGI BY PY-GC-MS AND VOC ANALYSIS

UDC 630.844.2

  • Olga Alexandrovna Shapchenkova V.N. Sukachev Institute of Forest SB RAS - separate subdivision of the Federal Research Center «Krasnoyarsk Science Center SB RAS» Email: shapchenkova@mail.ru
  • Antonina Alexandrovna Aniskina V.N. Sukachev Institute of Forest SB RAS - separate subdivision of the Federal Research Center «Krasnoyarsk Science Center SB RAS» Email: aniskina_a@ksc.krasn.ru
  • Nataliya Veniaminova Pashenova V.N. Sukachev Institute of Forest SB RAS - separate subdivision of the Federal Research Center «Krasnoyarsk Science Center SB RAS» Email: pasnat@ksc.krasn.ru
  • Sergey Redzhinaldovich Loskutov V.N. Sukachev Institute of Forest SB RAS - separate subdivision of the Federal Research Center «Krasnoyarsk Science Center SB RAS» Email: lsr@ksc.krasn.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.20240312536
Keywords: fungi, mycelia, Py-GC/MS, GC/MS, pyrolysates, VOCs

Abstract

Pathogenic fungi, along with fires and insect pests, are among the most important factors affecting coniferous forests in Siberia. Conifers are attacked by fungi that cause stains and decay of wood. The decrease in quality and timber loss due to stain and decay caused by fungi can be significant. The early detection of fungi in wood allows taking preventive measures to reduce the potential threats caused by fungi in the forests. The mycelia of brown-rot (Fomitopsis betulina, Phaeolus schweinitzii) and white-rot (Trametes versicolor, Phellinus chrysoloma) fungi and mycelia extracts were studied using methods of pyrolysis-gas chromatography-mass spectrometry (Py-GC/MS) and GC/MS, respectively, to find out whether the chemical compounds can be useful as fungal markers. Py-GC/MS of mycelia showed pyrolysis products of glucans, chitin, chitosan, proteins, and lipids. Volatile organic compounds (VOCs) of mycelia extracts were represented by 14-28 individual volatile compounds: Phaeolus schweinitzii> Phellinus chrysoloma> Fomitopsis betulina> Trametes versicolor. The presence of N-bearing compounds, 1-octene, 1-decanol among pyrolysis products of wood and some fungal VOCs emitted from wood may be indicative of fungi. The usefulness of the markers detected needs to be further confirmed by examining wood infected by these fungi.

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Author Biographies

Olga Alexandrovna Shapchenkova , V.N. Sukachev Institute of Forest SB RAS - separate subdivision of the Federal Research Center «Krasnoyarsk Science Center SB RAS»

PhD, Senior researcher

Antonina Alexandrovna Aniskina , V.N. Sukachev Institute of Forest SB RAS - separate subdivision of the Federal Research Center «Krasnoyarsk Science Center SB RAS»

Research assistant

Nataliya Veniaminova Pashenova , V.N. Sukachev Institute of Forest SB RAS - separate subdivision of the Federal Research Center «Krasnoyarsk Science Center SB RAS»

PhD, Senior researcher

Sergey Redzhinaldovich Loskutov , V.N. Sukachev Institute of Forest SB RAS - separate subdivision of the Federal Research Center «Krasnoyarsk Science Center SB RAS»

Doctor of Chemical Sciences, Fellow of the IAWS

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Published
2024-09-30
How to Cite
1. Shapchenkova O. A., Aniskina A. A., Pashenova N. V., Loskutov S. R. IDENTIFICATION OF MARKERS FOR MYCELIA OF WHITE-ROT AND BROWN-ROT FUNGI BY PY-GC-MS AND VOC ANALYSIS // chemistry of plant raw material, 2024. № 3. P. 273-279. URL: http://journal.asu.ru/cw/article/view/12536.
Issue
No 3 (2024)
Section
Low-molecular weight compounds

Copyright (c) 2024 chemistry of plant raw material

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