CONTENT OF CATECHINS IN LEAVES AND ROOTS OF COMARUM SALESOVIANUM AND COMARUM PALUSTRE (ROSACEAE)

UDK 582.734: 615.322

  • Татьяна Абдулхаиловна Kukushkina Central Siberian Botanical Gardens SB RAS https://orcid.org/0000-0001-8870-6101 Email: kukushkina-phyto@yandex.ru
  • Vera Andreevna Kostikova Central Siberian Botanical Garden SB RAS Email: serebryakovava@mail.ru
  • Elena Petrovna Khramova Central Siberian Botanical Gardens SB RAS https://orcid.org/0000-0002-8430-4575 Email: elenakhramova2023@yandex.ru
Keywords: Comarum, catechins, spectrophotometry, HPLC, validation

Abstract

The search for additional sources of catechins among the local flora is relevant today. The method for determining the content of catechins by the spectrophotometric method in the leaves of the subshrub Zalesov's cinquefoil (Comarum salesovianum (Steph.) Asch. et Graebn.) from the Rosaceae Juss. family was validated. The content of catechins in terms of (±)-catechin in the leaves and rhizomes of two species of the genus ComarumC. salesovianum and C. palustre L. (marsh cinquefoil) was studied. The highest content of catechins was found in the roots of C. palustre (4%) and leaves of C. salesovianum (2.58%). A method for determining the composition and content of individual catechins in the leaves and roots of two representatives of the genus Comarum by high-performance liquid chromatography has been proposed. Three catechins [(±)-catechin, epigallocatechin gallate and (-)-epicatechin] were found in leaves and roots. (±)-catechin and (-)-epicatechin are predominant in the leaves and roots of C. palustre and C. salesovianum. The content of epigallocatechin gallate does not exceed 0.1 mg/g. The concentration of (±)-catechin is highest in the roots (1.21 mg/g) of C. palustre, and (-)-epicatechin is highest in the leaves (1.35 mg/g) of C. salesovianum.

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Author Biographies

Татьяна Абдулхаиловна Kukushkina, Central Siberian Botanical Gardens SB RAS

senior researcher

Vera Andreevna Kostikova , Central Siberian Botanical Garden SB RAS

candidate of biological sciences, senior researcher

Elena Petrovna Khramova, Central Siberian Botanical Gardens SB RAS

Doctor of Biological Sciences, сhief researcher, head of the laboratory of phytochemistry

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Published
2024-05-22
How to Cite
1. KukushkinaТ. А., Kostikova V. A., Khramova E. P. CONTENT OF CATECHINS IN LEAVES AND ROOTS OF COMARUM SALESOVIANUM AND COMARUM PALUSTRE (ROSACEAE) // chemistry of plant raw material, 2024. № 2. P. 196-206. URL: http://journal.asu.ru/cw/article/view/12561.
Section
Low-molecular weight compounds