DEVELOPMENT OF METHODS FOR QUALITATIVE IDENTIFICATION AND QUANTITATIVE DETERMINATION OF THE AMOUNT OF FLAVONOIDS IN THE HERB CORONILLA VARIA L. (CORONILLA VARIA L.)

UDK 615.322:58

  • Tatyana Vladimirovna Sen Belgorod State National Research University Email: Sen_T@bsu.edu.ru
  • Valentina Nikolaevna Bubenchikova Kursk State Medical University Email: bubenhikova.ksmu@yandex.ru
  • Ksenia Romanovna Bubenchikova Kursk State Medical University Email: bubenhikova.ksmu@yandex.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.20240212647
Keywords: Coronilla varia, herb, qualitative identification of flavonoids, spectrophotometric determination of the sum of flavonoids

Abstract

Annotation. Coronilla varia is a perennial herbaceous plant, widely represented in the regions of the Central Black Earth Region, the Caucasus and Siberia. Various parts of the plant are used in folk medicine to treat diathesis, diarrhea, prolapse of the stomach, and as a cardiac and diuretic. The chemical composition of the herb elm variegated is not sufficiently studied. The poorly studied classes of biologically active substances of elm grass include flavonoid compounds.

The aim of the work is to develop methods of qualitative identification and quantitative determination of flavonoids in the herb Coronilla varia.

The object of the study was selected Coronilla varia herb, which was harvested in 2020-2022 in the Belgorod region, cutting the above-ground part at the level of 30 cm. Optimal conditions for qualitative identification of flavonoids were determined: extraction of raw materials - 10 minutes: extractant - ethyl alcohol 96%, plates used: "Sorbfil PTSH-AF-A-UF 10x20", solvent system: butanol-acetic acid-water (4:1:2), volume of applied extraction 10 µl, chamber saturation time - 30 minutes, detecting agent - 5% aluminum chloride solution.

Quantitative analysis of flavonoids was carried out using differential spectrophotometry. The method of quantitative determination was developed in the following directions: study of extraction parameters on flavonoids yield from the given raw material, determination of absorption maxima of extraction from raw material with aluminum chloride, study of spectrophotometric conditions of obtained complex, choice of standard substance. Crushing degree of raw materials 1 mm, extractant - ethyl alcohol 70%, extraction time 60 minutes, ratio raw material-extractant (1:100), the standard substance hyperoside.

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Author Biographies

Tatyana Vladimirovna Sen, Belgorod State National Research University

Candidate of Pharmaceutical Sciences, Associate Professor of the Department of Pharmaceutical Technology

Valentina Nikolaevna Bubenchikova , Kursk State Medical University

Doctor of Pharmaceutical Sciences, Head of the Department of Pharmacognosy and Botany

Ksenia Romanovna Bubenchikova, Kursk State Medical University

student

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Published
2024-06-01
How to Cite
1. Sen T. V., Bubenchikova V. N., Bubenchikova K. R. DEVELOPMENT OF METHODS FOR QUALITATIVE IDENTIFICATION AND QUANTITATIVE DETERMINATION OF THE AMOUNT OF FLAVONOIDS IN THE HERB CORONILLA VARIA L. (CORONILLA VARIA L.) // chemistry of plant raw material, 2024. № 2. P. 237-244. URL: http://journal.asu.ru/cw/article/view/12647.
Issue
No 2 (2024)
Section
Low-molecular weight compounds

Copyright (c) 2024 chemistry of plant raw material

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