EFFICIENCY OF CONVERSION BY A COMPLEX OF HYDROLYTIC ENZYMES OF WHEAT BRAN BIOPOLYMERS
UDK 664.764:664.162.036.1-044.3
Abstract
Introduction. An important aspect of the processing and pre-processing of cellulose raw materials (including bran) is to obtain a high content of reducing substances in the final product. Experimentally selected process parameters and optimization of pre-processing conditions of plant raw materials, in order to increase the amount of biologically valuable substances, will reduce the cost of the final product. In this work, the bioconversion of wheat bran polymers was carried out with hydrolytic enzyme preparations (ЕР).
Study objects and methods. The degree of biotransformation of plant polymers was evaluated on crushed wheat bran with enzyme preparations and their complexes by chemical analysis and HPLC chromatography.
Results and discussion The feedstock (wheat bran) was characterized by a low content of lignin (7.55%) and high pentosans (17.9%). The largest amount of reducing substances of hydrolysates was determined for ЕР Amilolux ATS – 0.23 g/g of raw materials, and its complexes Amilolux ATS and Celolux A – 0.29 g/g of raw materials. The inclusion of proteolytic action in the ЕР complex increases the amount of amine nitrogen (39.5 mg /g), reduces kinematic viscosity. A greater amount of mannose (56.0 mg/g of bran), but less pentose (4.1 mg/g of bran) of hydrolysates of enzymatically processed wheat bran was determined in comparison with chemical treatment.
Conclusions. Optimal parameters of enzymatic pretreatment of wheat bran for their conversion into target products of biosynthesis – biologically valuable carbohydrates have been determined, which is a promising direction of research and their practical use in the production of mannose, biofuels, chemicals and food additive.
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