QUANTITATIVE DETERMINATION OF GLYCOALKALOIDS IN POTATO TUBERS OF VARIETIES "KREPYSH" AND "ROZARA" WHEN CULTIVATED IN THE CONDITIONS OF THE MIDDLE TAIGA SUBZONE OF THE REPUBLIC OF KOMI

UDK 543.054; 543.544.5.068.7; 543.062

  • Vasily Vitalievich Punegov Institute of Biology, Federal Research Center Komi Scientific Center, Ural Branch of the Russian Academy of Sciences Email: punegov@ib.komisc.ru
  • Ivan Vladimirovich Gruzdev Institute of Biology, Komi Scientific Center, Ural Branch of the Russian Academy of Sciences Email: gruzdev@ib.komisc.ru
  • Sergey Aleksadrovich Patov Institute of Chemistry, Komi Scientific Center, Ural Branch of the Russian Academy of Sciences Email: ser-patov@yandex.ru
  • Olga Valerievna Skrotskaya Institute of Biology, Komi Scientific Center, Ural Branch of the Russian Academy of Sciences Email: skrockaja@ib.komisc.ru
  • Konstantin Vithalievich Chucha Institute of Biology, Komi Scientific Center, Ural Branch of the Russian Academy of Sciences Email: v.punegow@yandex.ru
DOI_disc_logo https://doi.org/10.14258/jcprm.20240213158
Keywords: cultivars Solanum tuberosum L., glycoalkaloids, α-solanine, α-chaconine, solanidine, acid hydrolysis, extraction, gravimetric analysis, GC-MS, HPLC

Abstract

The aim of the work was the quantitative determination of glycoalkaloids (GLA) in two varieties of potatoes - Krepysh and Rosara after long-term storage of the crop. It was planned to solve the following tasks: to extract the amount of GLA from samples of potato tubers of the indicated varieties, to determine the presence of GLA in the extract with the identification of solanidine aglycone, to perform a quantitative analysis of the mass fraction of α-chaconine and α-solanine in raw plant material by HPLC analysis. Hydrolytic extraction was used to isolate the GLA fraction of potato varieties Krepysh and Rozara grown in the middle taiga subzone of the Komi Republic. Solanidine, an aglycone of potato GLA, was identified by GLC-MS analysis in both cultivars. The HPLC method determined the content of individual GLA. In potatoes of the Krepysh variety, after six months of storage, the content of α-chaconine in terms of fresh raw materials is 0.0160±0.0018%, α-salonine is 0.0058±0.0007%, in potatoes of the Rosara variety, the content of α-chaconine is 0.0159±0.0018%, α-salonine – 0.0068±0.0008%. Both potato varieties when grown in the middle taiga subzone of the Komi Republic after a five-month storage of the crop are characterized by a slight excess of GLA content in tubers, and therefore the Krepysh variety should be recommended for further study of its ecological and biological characteristics in the conditions of the North as part of the scientific collection of the Botanical Garden of the Institute of Biology.

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Author Biographies

Vasily Vitalievich Punegov , Institute of Biology, Federal Research Center Komi Scientific Center, Ural Branch of the Russian Academy of Sciences

Candidate of Chemical Sciences, senior researcher at the Botanical Garden department

Ivan Vladimirovich Gruzdev, Institute of Biology, Komi Scientific Center, Ural Branch of the Russian Academy of Sciences

Doctor of Chemical Sciences, Associate Professor, Leading Researcher of the Ecoanalytical Laboratory

Sergey Aleksadrovich Patov, Institute of Chemistry, Komi Scientific Center, Ural Branch of the Russian Academy of Sciences

Candidate of Chemical Sciences, researcher at the Laboratory of Physical and Chemical Research Methods

Olga Valerievna Skrotskaya, Institute of Biology, Komi Scientific Center, Ural Branch of the Russian Academy of Sciences

Candidate of Biological Sciences, Associate Professor, Head of Department Botanical Garden

Konstantin Vithalievich Chucha, Institute of Biology, Komi Scientific Center, Ural Branch of the Russian Academy of Sciences

engineer of the first category of the Botanical Garden department

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Published
2024-06-17
How to Cite
1. Punegov V. V., Gruzdev I. V., Patov S. A., Skrotskaya O. V., Chucha K. V. QUANTITATIVE DETERMINATION OF GLYCOALKALOIDS IN POTATO TUBERS OF VARIETIES "KREPYSH" AND "ROZARA" WHEN CULTIVATED IN THE CONDITIONS OF THE MIDDLE TAIGA SUBZONE OF THE REPUBLIC OF KOMI // chemistry of plant raw material, 2024. № 2. P. 302-309. URL: http://journal.asu.ru/cw/article/view/13158.
Issue
No 2 (2024)
Section
Low-molecular weight compounds

Copyright (c) 2024 chemistry of plant raw material

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